Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.

In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection aga...

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Autores principales:	Steven E A Christiaen, Mary O'Connell Motherway, Francesca Bottacini, Noreen Lanigan, Pat G Casey, Geert Huys, Hans J Nelis, Douwe van Sinderen, Tom Coenye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20e1d509e7df4b529cc9de09d620e7b4
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spelling	oai:doaj.org-article:20e1d509e7df4b529cc9de09d620e7b42021-11-18T08:17:56ZAutoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.1932-620310.1371/journal.pone.0098111https://doaj.org/article/20e1d509e7df4b529cc9de09d620e7b42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24871429/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.Steven E A ChristiaenMary O'Connell MotherwayFrancesca BottaciniNoreen LaniganPat G CaseyGeert HuysHans J NelisDouwe van SinderenTom CoenyePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98111 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Steven E A Christiaen Mary O'Connell Motherway Francesca Bottacini Noreen Lanigan Pat G Casey Geert Huys Hans J Nelis Douwe van Sinderen Tom Coenye Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
description	In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.
format	article
author	Steven E A Christiaen Mary O'Connell Motherway Francesca Bottacini Noreen Lanigan Pat G Casey Geert Huys Hans J Nelis Douwe van Sinderen Tom Coenye
author_facet	Steven E A Christiaen Mary O'Connell Motherway Francesca Bottacini Noreen Lanigan Pat G Casey Geert Huys Hans J Nelis Douwe van Sinderen Tom Coenye
author_sort	Steven E A Christiaen
title	Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
title_short	Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
title_full	Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
title_fullStr	Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
title_full_unstemmed	Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
title_sort	autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of bifidobacterium breve ucc2003.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/20e1d509e7df4b529cc9de09d620e7b4
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Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.

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