Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression.
Tumour-specific splicing is known to contribute to cancer progression. In the case of the L1 cell adhesion molecule (L1CAM), which is expressed in many human tumours and often linked to bad prognosis, alternative splicing results in a full-length form (FL-L1CAM) and a splice variant lacking exons 2...
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2011
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oai:doaj.org-article:20e94dbc38a8494ea8dea37e22a7e4452021-11-18T06:55:15ZFull-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression.1932-620310.1371/journal.pone.0018989https://doaj.org/article/20e94dbc38a8494ea8dea37e22a7e4452011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541352/?tool=EBIhttps://doaj.org/toc/1932-6203Tumour-specific splicing is known to contribute to cancer progression. In the case of the L1 cell adhesion molecule (L1CAM), which is expressed in many human tumours and often linked to bad prognosis, alternative splicing results in a full-length form (FL-L1CAM) and a splice variant lacking exons 2 and 27 (SV-L1CAM). It has not been elucidated so far whether SV-L1CAM, classically considered as tumour-associated, or whether FL-L1CAM is the metastasis-promoting isoform. Here, we show that both variants were expressed in human ovarian carcinoma and that exposure of tumour cells to pro-metastatic factors led to an exclusive increase of FL-L1CAM expression. Selective overexpression of one isoform in different tumour cells revealed that only FL-L1CAM promoted experimental lung and/or liver metastasis in mice. In addition, metastasis formation upon up-regulation of FL-L1CAM correlated with increased invasive potential and elevated Matrix metalloproteinase (MMP)-2 and -9 expression and activity in vitro as well as enhanced gelatinolytic activity in vivo. In conclusion, we identified FL-L1CAM as the metastasis-promoting isoform, thereby exemplifying that high expression of a so-called tumour-associated variant, here SV-L1CAM, is not per se equivalent to a decisive role of this isoform in tumour progression.Stephanie HauserLaura BickelDirk WeinspachMichael GergMichael K SchäferMarco PfeiferJohn HazinFlorian SchelterUlrich H WeidleJuliane RamserJuliane VolkmannAlfons MeindlManfred SchmittFlorian SchrötzlmairPeter AltevogtAchim KrügerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18989 (2011) |
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Medicine R Science Q Stephanie Hauser Laura Bickel Dirk Weinspach Michael Gerg Michael K Schäfer Marco Pfeifer John Hazin Florian Schelter Ulrich H Weidle Juliane Ramser Juliane Volkmann Alfons Meindl Manfred Schmitt Florian Schrötzlmair Peter Altevogt Achim Krüger Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| description |
Tumour-specific splicing is known to contribute to cancer progression. In the case of the L1 cell adhesion molecule (L1CAM), which is expressed in many human tumours and often linked to bad prognosis, alternative splicing results in a full-length form (FL-L1CAM) and a splice variant lacking exons 2 and 27 (SV-L1CAM). It has not been elucidated so far whether SV-L1CAM, classically considered as tumour-associated, or whether FL-L1CAM is the metastasis-promoting isoform. Here, we show that both variants were expressed in human ovarian carcinoma and that exposure of tumour cells to pro-metastatic factors led to an exclusive increase of FL-L1CAM expression. Selective overexpression of one isoform in different tumour cells revealed that only FL-L1CAM promoted experimental lung and/or liver metastasis in mice. In addition, metastasis formation upon up-regulation of FL-L1CAM correlated with increased invasive potential and elevated Matrix metalloproteinase (MMP)-2 and -9 expression and activity in vitro as well as enhanced gelatinolytic activity in vivo. In conclusion, we identified FL-L1CAM as the metastasis-promoting isoform, thereby exemplifying that high expression of a so-called tumour-associated variant, here SV-L1CAM, is not per se equivalent to a decisive role of this isoform in tumour progression. |
| format |
article |
| author |
Stephanie Hauser Laura Bickel Dirk Weinspach Michael Gerg Michael K Schäfer Marco Pfeifer John Hazin Florian Schelter Ulrich H Weidle Juliane Ramser Juliane Volkmann Alfons Meindl Manfred Schmitt Florian Schrötzlmair Peter Altevogt Achim Krüger |
| author_facet |
Stephanie Hauser Laura Bickel Dirk Weinspach Michael Gerg Michael K Schäfer Marco Pfeifer John Hazin Florian Schelter Ulrich H Weidle Juliane Ramser Juliane Volkmann Alfons Meindl Manfred Schmitt Florian Schrötzlmair Peter Altevogt Achim Krüger |
| author_sort |
Stephanie Hauser |
| title |
Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| title_short |
Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| title_full |
Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| title_fullStr |
Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| title_full_unstemmed |
Full-length L1CAM and not its Δ2Δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| title_sort |
full-length l1cam and not its δ2δ27 splice variant promotes metastasis through induction of gelatinase expression. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/20e94dbc38a8494ea8dea37e22a7e445 |
| work_keys_str_mv |
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