First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract

Abstract This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G pro...

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Autores principales:	Jessica Calo, Ayelén M. Blanco, Sara Comesaña, Marta Conde-Sieira, Sofia Morais, José L. Soengas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20eb3de8a94348b9b00b8a853a9fb3d1
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spelling	oai:doaj.org-article:20eb3de8a94348b9b00b8a853a9fb3d12021-12-02T13:34:45ZFirst evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract10.1038/s41598-021-84303-92045-2322https://doaj.org/article/20eb3de8a94348b9b00b8a853a9fb3d12021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84303-9https://doaj.org/toc/2045-2322Abstract This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G protein-coupled receptor family C group 6 member A (GPRC6A), and taste receptors type 1 members 1 and 2 (T1r1, T1r2)], while others [taste receptor type 1 member 3 (T1r3) and metabotropic glutamate receptors 1 and 4 (mGlur1, mGlur4)] could not be found. Then, we characterized the response of such receptors, as well as that of intracellular signaling mechanisms, to the intragastric administration of l-leucine, l-valine, l-proline or l-glutamate. Results demonstrated that casr, gprc6a, tas1r1 and tas1r2 mRNAs are modulated by amino acids in the stomach and proximal intestine, with important differences with respect to mammals. Likewise, gut amino acid receptors triggered signaling pathways likely mediated, at least partly, by phospholipase C β3 and β4. Finally, the luminal presence of amino acids led to important changes in ghrelin, cholecystokinin, peptide YY and proglucagon mRNAs and/or protein levels. Present results offer the first set of evidence in favor of the existence of amino acid sensing mechanisms within the fish GIT.Jessica CaloAyelén M. BlancoSara ComesañaMarta Conde-SieiraSofia MoraisJosé L. SoengasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica Calo Ayelén M. Blanco Sara Comesaña Marta Conde-Sieira Sofia Morais José L. Soengas First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
description	Abstract This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G protein-coupled receptor family C group 6 member A (GPRC6A), and taste receptors type 1 members 1 and 2 (T1r1, T1r2)], while others [taste receptor type 1 member 3 (T1r3) and metabotropic glutamate receptors 1 and 4 (mGlur1, mGlur4)] could not be found. Then, we characterized the response of such receptors, as well as that of intracellular signaling mechanisms, to the intragastric administration of l-leucine, l-valine, l-proline or l-glutamate. Results demonstrated that casr, gprc6a, tas1r1 and tas1r2 mRNAs are modulated by amino acids in the stomach and proximal intestine, with important differences with respect to mammals. Likewise, gut amino acid receptors triggered signaling pathways likely mediated, at least partly, by phospholipase C β3 and β4. Finally, the luminal presence of amino acids led to important changes in ghrelin, cholecystokinin, peptide YY and proglucagon mRNAs and/or protein levels. Present results offer the first set of evidence in favor of the existence of amino acid sensing mechanisms within the fish GIT.
format	article
author	Jessica Calo Ayelén M. Blanco Sara Comesaña Marta Conde-Sieira Sofia Morais José L. Soengas
author_facet	Jessica Calo Ayelén M. Blanco Sara Comesaña Marta Conde-Sieira Sofia Morais José L. Soengas
author_sort	Jessica Calo
title	First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
title_short	First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
title_full	First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
title_fullStr	First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
title_full_unstemmed	First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
title_sort	first evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/20eb3de8a94348b9b00b8a853a9fb3d1
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First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract

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