A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.

<h4>Purpose</h4>To introduce a constant-force technique for the analysis of corneal biomechanical changes induced after collagen cross-linking (CXL) that is better adapted to the natural loading in the eye than previous methods.<h4>Methods</h4>For the biomechanical testing, a...

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Autores principales:	Olivier Richoz, Sabine Kling, Souska Zandi, Arthur Hammer, Eberhard Spoerl, Farhad Hafezi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20f32e8431464bb5a45253dd68612ea6
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spelling	oai:doaj.org-article:20f32e8431464bb5a45253dd68612ea62021-11-25T06:02:58ZA constant-force technique to measure corneal biomechanical changes after collagen cross-linking.1932-620310.1371/journal.pone.0105095https://doaj.org/article/20f32e8431464bb5a45253dd68612ea62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25162621/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>To introduce a constant-force technique for the analysis of corneal biomechanical changes induced after collagen cross-linking (CXL) that is better adapted to the natural loading in the eye than previous methods.<h4>Methods</h4>For the biomechanical testing, a total of 50 freshly enucleated eyes were obtained and subdivided in groups of 5 eyes each. A Zwicki-Line Testing Machine was used to analyze the strain of 11 mm long and 5 mm wide porcine corneal strips, with and without CXL. Before material testing, the corneal tissues were pre-stressed with 0.02 N until force stabilization. Standard strip extensiometry was performed as control technique. For the constant-force technique, tissue elongation (Δ strain, %) was analyzed for 180 seconds while different constant forces (0.25 N, 0.5 N, 1 N, 5 N) were applied.<h4>Results</h4>Using a constant force of 0.5 N, we observed a significant difference in Δstrain between 0.26±0.01% in controls and 0.12±0.03% in the CXL-treated group (p = 0.003) over baseline. Similarly, using a constant force of 1 N, Δstrain was 0.31±0.03% in controls and 0.19±0.02% after CXL treatment (p = 0.008). No significant differences were observed between CXL-treated groups and controls with 0.25 N or 5 N constant forces. Standard stress-strain extensiometry failed to show significant differences between CXL-treated groups and controls at all percentages of strains tested.<h4>Conclusion</h4>We propose a constant-force technique to measure corneal biomechanics in a more physiologic way. When compared to standard stress-strain extensiometry, the constant-force technique provides less variability and thus reaches significant results with a lower sample number.Olivier RichozSabine KlingSouska ZandiArthur HammerEberhard SpoerlFarhad HafeziPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105095 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olivier Richoz Sabine Kling Souska Zandi Arthur Hammer Eberhard Spoerl Farhad Hafezi A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
description	<h4>Purpose</h4>To introduce a constant-force technique for the analysis of corneal biomechanical changes induced after collagen cross-linking (CXL) that is better adapted to the natural loading in the eye than previous methods.<h4>Methods</h4>For the biomechanical testing, a total of 50 freshly enucleated eyes were obtained and subdivided in groups of 5 eyes each. A Zwicki-Line Testing Machine was used to analyze the strain of 11 mm long and 5 mm wide porcine corneal strips, with and without CXL. Before material testing, the corneal tissues were pre-stressed with 0.02 N until force stabilization. Standard strip extensiometry was performed as control technique. For the constant-force technique, tissue elongation (Δ strain, %) was analyzed for 180 seconds while different constant forces (0.25 N, 0.5 N, 1 N, 5 N) were applied.<h4>Results</h4>Using a constant force of 0.5 N, we observed a significant difference in Δstrain between 0.26±0.01% in controls and 0.12±0.03% in the CXL-treated group (p = 0.003) over baseline. Similarly, using a constant force of 1 N, Δstrain was 0.31±0.03% in controls and 0.19±0.02% after CXL treatment (p = 0.008). No significant differences were observed between CXL-treated groups and controls with 0.25 N or 5 N constant forces. Standard stress-strain extensiometry failed to show significant differences between CXL-treated groups and controls at all percentages of strains tested.<h4>Conclusion</h4>We propose a constant-force technique to measure corneal biomechanics in a more physiologic way. When compared to standard stress-strain extensiometry, the constant-force technique provides less variability and thus reaches significant results with a lower sample number.
format	article
author	Olivier Richoz Sabine Kling Souska Zandi Arthur Hammer Eberhard Spoerl Farhad Hafezi
author_facet	Olivier Richoz Sabine Kling Souska Zandi Arthur Hammer Eberhard Spoerl Farhad Hafezi
author_sort	Olivier Richoz
title	A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
title_short	A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
title_full	A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
title_fullStr	A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
title_full_unstemmed	A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
title_sort	constant-force technique to measure corneal biomechanical changes after collagen cross-linking.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/20f32e8431464bb5a45253dd68612ea6
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A constant-force technique to measure corneal biomechanical changes after collagen cross-linking.

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