Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq

Hippocampal sclerosis (HS) is one of the most common pathological type of intractable temporal lobe epilepsy (TLE), often characterized by hippocampal atrophy, neuronal apoptosis, and gliogenesis. However, the molecular mechanisms of neuronal apoptosis in patients with HS are still not fully underst...

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Autores principales:	Shengkun Yu, Yifei Gu, Tianyu Wang, Long Mu, Haiyang Wang, Shi Yan, Aoweng Wang, Jiabin Wang, Li Liu, Hong Shen, Meng Na, Zhiguo Lin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miRNAs ceRNA temporal lobe epilepsy hippocampal sclerosis lncRNAs Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/20f3fe35dd804c769a94b17090c22e9a
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spelling	oai:doaj.org-article:20f3fe35dd804c769a94b17090c22e9a2021-11-12T07:03:26ZStudy of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq1662-453X10.3389/fnins.2021.770627https://doaj.org/article/20f3fe35dd804c769a94b17090c22e9a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.770627/fullhttps://doaj.org/toc/1662-453XHippocampal sclerosis (HS) is one of the most common pathological type of intractable temporal lobe epilepsy (TLE), often characterized by hippocampal atrophy, neuronal apoptosis, and gliogenesis. However, the molecular mechanisms of neuronal apoptosis in patients with HS are still not fully understood. We therefore conducted a pilot study focusing on the neuronal apoptosis ceRNA network in the sclerotic hippocampus of intractable TLE patients. In this research, RNA sequencing (RNA-seq) was utilized to quantify the expression levels of lncRNAs, miRNAs, and mRNAs in TLE patients with HS (HS-TLE) and without HS (non-HS-TLE), and reverse transcription-quantitative PCR (qRT-PCR). The interactions of differential expression (DE) lncRNAs-miRNAs or DEmiRNAs-mRNAs were integrated by StarBase v3.0, and visualized using Cytoscape. Subsequently, we annotate the functions of lncRNA-associated competitive endogenous RNA (ceRNA) network through analysis of their interactions with mRNAs. RNA-seq analyses showed 381 lncRNAs, 42 miRNAs, and 457 mRNAs were dysregulated expression in HS-TLE compared to non-HS-TLE. According to the ceRNA hypothesis, 5 HS-specific ceRNA network were constructed. Among them, the core ceRNA regulatory network involved in neuronal apoptosis was constituted by 10 DElncRNAs (CDKN2B-AS1, MEG3, UBA6-AS1, etc.), 7 DEmiRNAs (hsa-miR-155-5p, hsa-miR-195-5p, hsa-miR-200c-3p, etc.), and 3 DEmRNAs (SCN2A, DYRK2, and MAPK8), which belonging to apoptotic and epileptic terms. Our findings established the first ceRNA network of lncRNA-mediated neuronal apoptosis in HS-TLE based on transcriptome sequencing, which provide a new perspective on the disease pathogenesis and precise treatments of HS.Shengkun YuYifei GuTianyu WangLong MuHaiyang WangShi YanAoweng WangJiabin WangLi LiuHong ShenMeng NaZhiguo LinFrontiers Media S.A.articlemiRNAsceRNAtemporal lobe epilepsyhippocampal sclerosislncRNAsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNAs ceRNA temporal lobe epilepsy hippocampal sclerosis lncRNAs Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	miRNAs ceRNA temporal lobe epilepsy hippocampal sclerosis lncRNAs Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Shengkun Yu Yifei Gu Tianyu Wang Long Mu Haiyang Wang Shi Yan Aoweng Wang Jiabin Wang Li Liu Hong Shen Meng Na Zhiguo Lin Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
description	Hippocampal sclerosis (HS) is one of the most common pathological type of intractable temporal lobe epilepsy (TLE), often characterized by hippocampal atrophy, neuronal apoptosis, and gliogenesis. However, the molecular mechanisms of neuronal apoptosis in patients with HS are still not fully understood. We therefore conducted a pilot study focusing on the neuronal apoptosis ceRNA network in the sclerotic hippocampus of intractable TLE patients. In this research, RNA sequencing (RNA-seq) was utilized to quantify the expression levels of lncRNAs, miRNAs, and mRNAs in TLE patients with HS (HS-TLE) and without HS (non-HS-TLE), and reverse transcription-quantitative PCR (qRT-PCR). The interactions of differential expression (DE) lncRNAs-miRNAs or DEmiRNAs-mRNAs were integrated by StarBase v3.0, and visualized using Cytoscape. Subsequently, we annotate the functions of lncRNA-associated competitive endogenous RNA (ceRNA) network through analysis of their interactions with mRNAs. RNA-seq analyses showed 381 lncRNAs, 42 miRNAs, and 457 mRNAs were dysregulated expression in HS-TLE compared to non-HS-TLE. According to the ceRNA hypothesis, 5 HS-specific ceRNA network were constructed. Among them, the core ceRNA regulatory network involved in neuronal apoptosis was constituted by 10 DElncRNAs (CDKN2B-AS1, MEG3, UBA6-AS1, etc.), 7 DEmiRNAs (hsa-miR-155-5p, hsa-miR-195-5p, hsa-miR-200c-3p, etc.), and 3 DEmRNAs (SCN2A, DYRK2, and MAPK8), which belonging to apoptotic and epileptic terms. Our findings established the first ceRNA network of lncRNA-mediated neuronal apoptosis in HS-TLE based on transcriptome sequencing, which provide a new perspective on the disease pathogenesis and precise treatments of HS.
format	article
author	Shengkun Yu Yifei Gu Tianyu Wang Long Mu Haiyang Wang Shi Yan Aoweng Wang Jiabin Wang Li Liu Hong Shen Meng Na Zhiguo Lin
author_facet	Shengkun Yu Yifei Gu Tianyu Wang Long Mu Haiyang Wang Shi Yan Aoweng Wang Jiabin Wang Li Liu Hong Shen Meng Na Zhiguo Lin
author_sort	Shengkun Yu
title	Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
title_short	Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
title_full	Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
title_fullStr	Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
title_full_unstemmed	Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq
title_sort	study of neuronal apoptosis cerna network in hippocampal sclerosis of human temporal lobe epilepsy by rna-seq
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/20f3fe35dd804c769a94b17090c22e9a
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Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq

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