Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients

Chiara Fariselli, Giuseppe Giannaccare, Michela Fresina, Piera Versura Ophthalmology Unit, Department of Experimental, Diagnostic and Specialty Medicine, School of Medicine (DIMES), Alma Mater Studiorum University of Bologna and St Orsola-Malpighi Teaching Hospital, Bologna, Italy Aim: To assess t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fariselli C, Giannaccare G, Fresina M, Versura P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Trehalose Sodium hyaluronate dry eye impression cytology MUC4 IL-1 IL-6 IL-8 Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/20f739d38ba8402c8c0cc6a95f35fbd1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:20f739d38ba8402c8c0cc6a95f35fbd1
record_format	dspace
spelling	oai:doaj.org-article:20f739d38ba8402c8c0cc6a95f35fbd12021-12-02T03:07:33ZTrehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients1177-5483https://doaj.org/article/20f739d38ba8402c8c0cc6a95f35fbd12018-07-01T00:00:00Zhttps://www.dovepress.com/trehalosehyaluronate-eyedrop-effects-on-ocular-surface-inflammatory-ma-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Chiara Fariselli, Giuseppe Giannaccare, Michela Fresina, Piera Versura Ophthalmology Unit, Department of Experimental, Diagnostic and Specialty Medicine, School of Medicine (DIMES), Alma Mater Studiorum University of Bologna and St Orsola-Malpighi Teaching Hospital, Bologna, Italy Aim: To assess the ocular surface parameters, inflammatory marker level in tears, and mucin expression in conjunctival epithelium before and after treatment with trehalose/hyaluronate tear substitute in dry eye (DE) patients.Patients and methods: Fifteen DE patients were evaluated in an open-label, pilot study at enrollment, after 2 days of washout (baseline) and after 1 and 2 months (endpoint) of treatment with a trehalose/hyaluronate tear substitute (one drop/eye/three times daily). Data for symptoms of discomfort (Ocular Surface Disease Index and Visual Analogue Scale pain score), tear film (Schirmer test I, tear film breakup time), ocular surface damage (corneal National Eye Institute) and conjunctival van Bijsterveld scores, impression cytology scored by Nelson’s grade and goblet cells (GCs) number/mm2 analysis, and MUC4 immunostaining, and inflammation (interleukin [IL]-1β, IL-6, and IL-8 levels) were measured.Results: Significant changes at endpoint as compared to baseline were found for Ocular Surface Disease Index score (respectively, mean±SD, 22.2±2.9 vs 38.7±12.7), Visual Analogue Scale score (3.4±1.3 vs 6.6±1.4), tear film breakup time (8.6±1.28 vs 6.17±1.9 seconds), corneal staining (National Eye Institute grade 1.23±0.64 vs 3.37±0.49), conjunctival staining (1.73±1.14 vs 4.17±0.91), impression cytology (Nelson grade 1.10±0.20 vs 1.63±0.54), and GC density (139.9±22.0 vs 107.8±16.2 GC/mm2). IL-1β, IL-6, and IL-8 tear levels showed a significant decrease at endpoint as compared to baseline (respectively, pg/mL tears: 12.3±6.9, 26.6±25.2, 743.5±477.7 vs 33.6±17.3, 112.0±24.3, 1,139.2±671.7).Conclusions: A decrease in ocular discomfort symptoms, surface damage, and tear cytokine levels was shown after 2 months’ treatment with trehalose/hyaluronate tear substitute in DE patients, along with a significant GC density recovery. These results may be associated with the synergic action of both trehalose and hyaluronic acid in targeting different entries of the DE vicious loop. Keywords: dry eye, trehalose, hyaluronic acid, tear substitute, inflammationFariselli CGiannaccare GFresina MVersura PDove Medical PressarticleTrehaloseSodium hyaluronatedry eyeimpression cytologyMUC4IL-1IL-6IL-8OphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 1293-1300 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trehalose Sodium hyaluronate dry eye impression cytology MUC4 IL-1 IL-6 IL-8 Ophthalmology RE1-994
spellingShingle	Trehalose Sodium hyaluronate dry eye impression cytology MUC4 IL-1 IL-6 IL-8 Ophthalmology RE1-994 Fariselli C Giannaccare G Fresina M Versura P Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
description	Chiara Fariselli, Giuseppe Giannaccare, Michela Fresina, Piera Versura Ophthalmology Unit, Department of Experimental, Diagnostic and Specialty Medicine, School of Medicine (DIMES), Alma Mater Studiorum University of Bologna and St Orsola-Malpighi Teaching Hospital, Bologna, Italy Aim: To assess the ocular surface parameters, inflammatory marker level in tears, and mucin expression in conjunctival epithelium before and after treatment with trehalose/hyaluronate tear substitute in dry eye (DE) patients.Patients and methods: Fifteen DE patients were evaluated in an open-label, pilot study at enrollment, after 2 days of washout (baseline) and after 1 and 2 months (endpoint) of treatment with a trehalose/hyaluronate tear substitute (one drop/eye/three times daily). Data for symptoms of discomfort (Ocular Surface Disease Index and Visual Analogue Scale pain score), tear film (Schirmer test I, tear film breakup time), ocular surface damage (corneal National Eye Institute) and conjunctival van Bijsterveld scores, impression cytology scored by Nelson’s grade and goblet cells (GCs) number/mm2 analysis, and MUC4 immunostaining, and inflammation (interleukin [IL]-1β, IL-6, and IL-8 levels) were measured.Results: Significant changes at endpoint as compared to baseline were found for Ocular Surface Disease Index score (respectively, mean±SD, 22.2±2.9 vs 38.7±12.7), Visual Analogue Scale score (3.4±1.3 vs 6.6±1.4), tear film breakup time (8.6±1.28 vs 6.17±1.9 seconds), corneal staining (National Eye Institute grade 1.23±0.64 vs 3.37±0.49), conjunctival staining (1.73±1.14 vs 4.17±0.91), impression cytology (Nelson grade 1.10±0.20 vs 1.63±0.54), and GC density (139.9±22.0 vs 107.8±16.2 GC/mm2). IL-1β, IL-6, and IL-8 tear levels showed a significant decrease at endpoint as compared to baseline (respectively, pg/mL tears: 12.3±6.9, 26.6±25.2, 743.5±477.7 vs 33.6±17.3, 112.0±24.3, 1,139.2±671.7).Conclusions: A decrease in ocular discomfort symptoms, surface damage, and tear cytokine levels was shown after 2 months’ treatment with trehalose/hyaluronate tear substitute in DE patients, along with a significant GC density recovery. These results may be associated with the synergic action of both trehalose and hyaluronic acid in targeting different entries of the DE vicious loop. Keywords: dry eye, trehalose, hyaluronic acid, tear substitute, inflammation
format	article
author	Fariselli C Giannaccare G Fresina M Versura P
author_facet	Fariselli C Giannaccare G Fresina M Versura P
author_sort	Fariselli C
title	Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
title_short	Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
title_full	Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
title_fullStr	Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
title_full_unstemmed	Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
title_sort	trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/20f739d38ba8402c8c0cc6a95f35fbd1
work_keys_str_mv	AT farisellic trehalosehyaluronateeyedropeffectsonocularsurfaceinflammatorymarkersandmucinexpressionindryeyepatients AT giannaccareg trehalosehyaluronateeyedropeffectsonocularsurfaceinflammatorymarkersandmucinexpressionindryeyepatients AT fresinam trehalosehyaluronateeyedropeffectsonocularsurfaceinflammatorymarkersandmucinexpressionindryeyepatients AT versurap trehalosehyaluronateeyedropeffectsonocularsurfaceinflammatorymarkersandmucinexpressionindryeyepatients
_version_	1718401905839833088

Trehalose/hyaluronate eyedrop effects on ocular surface inflammatory markers and mucin expression in dry eye patients

Ejemplares similares