A cell cycle role for the epigenetic factor CTCF-L/BORIS.

CTCF is a ubiquitous epigenetic regulator that has been proposed as a master keeper of chromatin organisation. CTCF-like, or BORIS, is thought to antagonise CTCF and has been found in normal testis, ovary and a large variety of tumour cells. The cellular function of BORIS remains intriguing although...

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Autores principales:	Manuel Rosa-Garrido, Laura Ceballos, Pilar Alonso-Lecue, Cristina Abraira, M Dolores Delgado, Alberto Gandarillas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20f77991f55d40a1ad7b5104476a4674
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spelling	oai:doaj.org-article:20f77991f55d40a1ad7b5104476a46742021-11-18T07:14:57ZA cell cycle role for the epigenetic factor CTCF-L/BORIS.1932-620310.1371/journal.pone.0039371https://doaj.org/article/20f77991f55d40a1ad7b5104476a46742012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22724006/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203CTCF is a ubiquitous epigenetic regulator that has been proposed as a master keeper of chromatin organisation. CTCF-like, or BORIS, is thought to antagonise CTCF and has been found in normal testis, ovary and a large variety of tumour cells. The cellular function of BORIS remains intriguing although it might be involved in developmental reprogramming of gene expression patterns. We here unravel the expression of CTCF and BORIS proteins throughout human epidermis. While CTCF is widely distributed within the nucleus, BORIS is confined to the nucleolus and other euchromatin domains. Nascent RNA experiments in primary keratinocytes revealed that endogenous BORIS is present in active transcription sites. Interestingly, BORIS also localises to interphase centrosomes suggesting a role in the cell cycle. Blocking the cell cycle at S phase or mitosis, or causing DNA damage, produced a striking accumulation of BORIS. Consistently, ectopic expression of wild type or GFP- BORIS provoked a higher rate of S phase cells as well as genomic instability by mitosis failure. Furthermore, down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis.Manuel Rosa-GarridoLaura CeballosPilar Alonso-LecueCristina AbrairaM Dolores DelgadoAlberto GandarillasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39371 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Rosa-Garrido Laura Ceballos Pilar Alonso-Lecue Cristina Abraira M Dolores Delgado Alberto Gandarillas A cell cycle role for the epigenetic factor CTCF-L/BORIS.
description	CTCF is a ubiquitous epigenetic regulator that has been proposed as a master keeper of chromatin organisation. CTCF-like, or BORIS, is thought to antagonise CTCF and has been found in normal testis, ovary and a large variety of tumour cells. The cellular function of BORIS remains intriguing although it might be involved in developmental reprogramming of gene expression patterns. We here unravel the expression of CTCF and BORIS proteins throughout human epidermis. While CTCF is widely distributed within the nucleus, BORIS is confined to the nucleolus and other euchromatin domains. Nascent RNA experiments in primary keratinocytes revealed that endogenous BORIS is present in active transcription sites. Interestingly, BORIS also localises to interphase centrosomes suggesting a role in the cell cycle. Blocking the cell cycle at S phase or mitosis, or causing DNA damage, produced a striking accumulation of BORIS. Consistently, ectopic expression of wild type or GFP- BORIS provoked a higher rate of S phase cells as well as genomic instability by mitosis failure. Furthermore, down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis.
format	article
author	Manuel Rosa-Garrido Laura Ceballos Pilar Alonso-Lecue Cristina Abraira M Dolores Delgado Alberto Gandarillas
author_facet	Manuel Rosa-Garrido Laura Ceballos Pilar Alonso-Lecue Cristina Abraira M Dolores Delgado Alberto Gandarillas
author_sort	Manuel Rosa-Garrido
title	A cell cycle role for the epigenetic factor CTCF-L/BORIS.
title_short	A cell cycle role for the epigenetic factor CTCF-L/BORIS.
title_full	A cell cycle role for the epigenetic factor CTCF-L/BORIS.
title_fullStr	A cell cycle role for the epigenetic factor CTCF-L/BORIS.
title_full_unstemmed	A cell cycle role for the epigenetic factor CTCF-L/BORIS.
title_sort	cell cycle role for the epigenetic factor ctcf-l/boris.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/20f77991f55d40a1ad7b5104476a4674
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A cell cycle role for the epigenetic factor CTCF-L/BORIS.

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