Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows

Abstract Heat stress negatively affects health and production in cows. Examining the cellular response to heat stress could reveal underlying protective molecular mechanisms associated with superior resilience and ultimately enable selection for more resilient cattle. This type of investigation is i...

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Autores principales:	A. M. Livernois, B. A. Mallard, S. L. Cartwright, A. Cánovas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20ff895afafe48f8b27b9fe45fde84f8
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spelling	oai:doaj.org-article:20ff895afafe48f8b27b9fe45fde84f82021-12-02T15:56:57ZHeat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows10.1038/s41598-021-89951-52045-2322https://doaj.org/article/20ff895afafe48f8b27b9fe45fde84f82021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89951-5https://doaj.org/toc/2045-2322Abstract Heat stress negatively affects health and production in cows. Examining the cellular response to heat stress could reveal underlying protective molecular mechanisms associated with superior resilience and ultimately enable selection for more resilient cattle. This type of investigation is increasingly important as future predictions for the patterns of heat waves point to increases in frequency, severity, and duration. Cows identified as high immune responders based on High Immune Response technology (HIR) have lower disease occurrence compared to their average and low immune responder herd-mates. In this study, our goal was to identify epigenetic differences between high and low immune responder cows in response to heat stress. We examined genome-wide DNA methylation of blood mononuclear cells (BMCs) isolated from high and low cows, before and after in vitro heat stress. We identified differential methylation of promoter regions associated with a variety of biological processes including immune function, stress response, apoptosis, and cell signalling. The specific differentially methylated promoter regions differed between samples from high and low cows, and results revealed pathways associated with cellular protection during heat stress.A. M. LivernoisB. A. MallardS. L. CartwrightA. CánovasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. M. Livernois B. A. Mallard S. L. Cartwright A. Cánovas Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
description	Abstract Heat stress negatively affects health and production in cows. Examining the cellular response to heat stress could reveal underlying protective molecular mechanisms associated with superior resilience and ultimately enable selection for more resilient cattle. This type of investigation is increasingly important as future predictions for the patterns of heat waves point to increases in frequency, severity, and duration. Cows identified as high immune responders based on High Immune Response technology (HIR) have lower disease occurrence compared to their average and low immune responder herd-mates. In this study, our goal was to identify epigenetic differences between high and low immune responder cows in response to heat stress. We examined genome-wide DNA methylation of blood mononuclear cells (BMCs) isolated from high and low cows, before and after in vitro heat stress. We identified differential methylation of promoter regions associated with a variety of biological processes including immune function, stress response, apoptosis, and cell signalling. The specific differentially methylated promoter regions differed between samples from high and low cows, and results revealed pathways associated with cellular protection during heat stress.
format	article
author	A. M. Livernois B. A. Mallard S. L. Cartwright A. Cánovas
author_facet	A. M. Livernois B. A. Mallard S. L. Cartwright A. Cánovas
author_sort	A. M. Livernois
title	Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
title_short	Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
title_full	Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
title_fullStr	Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
title_full_unstemmed	Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
title_sort	heat stress and immune response phenotype affect dna methylation in blood mononuclear cells from holstein dairy cows
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/20ff895afafe48f8b27b9fe45fde84f8
work_keys_str_mv	AT amlivernois heatstressandimmuneresponsephenotypeaffectdnamethylationinbloodmononuclearcellsfromholsteindairycows AT bamallard heatstressandimmuneresponsephenotypeaffectdnamethylationinbloodmononuclearcellsfromholsteindairycows AT slcartwright heatstressandimmuneresponsephenotypeaffectdnamethylationinbloodmononuclearcellsfromholsteindairycows AT acanovas heatstressandimmuneresponsephenotypeaffectdnamethylationinbloodmononuclearcellsfromholsteindairycows
_version_	1718385378299215872

Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows

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