Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria

Hepatopathy is frequently observed in patients with severe malaria but its pathogenesis remains unclear. Galectins are evolutionarily conserved glycan-binding proteins with pleiotropic roles in innate and adaptive immune responses, and exhibit pivotal roles during Plasmodium spp. infection. Here, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yifan Wu, Shiguang Huang, Siyu Xiao, Jian He, Fangli Lu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Plasmodium berghei liver macrophage Gal-9 Tim-3 IFNα Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/210407235459463b89ee383692d6f326
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:210407235459463b89ee383692d6f326
record_format	dspace
spelling	oai:doaj.org-article:210407235459463b89ee383692d6f3262021-11-30T18:45:48ZImpact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria1664-322410.3389/fimmu.2021.758052https://doaj.org/article/210407235459463b89ee383692d6f3262021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.758052/fullhttps://doaj.org/toc/1664-3224Hepatopathy is frequently observed in patients with severe malaria but its pathogenesis remains unclear. Galectins are evolutionarily conserved glycan-binding proteins with pleiotropic roles in innate and adaptive immune responses, and exhibit pivotal roles during Plasmodium spp. infection. Here, we analyzed the impact of blockage of galectin-receptor interactions by treatment with alpha (α)-lactose on liver immunopathology during the erythrocytic stage of malaria in mice infected with Plasmodium berghei ANKA (PbANKA). Our results found that compared with PbANKA-infected mice (malarial mice), blockage of galectin-receptor interactions led to decreased host survival rate and increased peripheral blood parasitemia; exacerbated liver pathology, increased numbers of CD68+ macrophages and apoptotic cells, and increased parasite burden in the livers on days 5 and 7 post infection (p.i.) as well as increased mRNA expression levels of galectin-9 (Gal-9) and its receptor, the T cell immunoglobulin domain and mucin domain protein 3 (Tim-3), interferon (IFN)α, IFNγ, and the triggering receptor expressed on myeloid cells (TREM)-1 in the livers or spleens of PbANKA-infected mice on day 7 p.i. Observed by transmission electron microscopy, the peritoneal macrophages isolated from malarial mice with α-lactose treatment had more pseudopodia than those from malarial mice. Measured by using quantitative real-time reverse transcription-polymerase chain reaction assay, the mRNA expression levels of Gal-9, IFNα, IFNβ, IFNγ, and TREM-1 were increased in the peritoneal macrophages isolated from malarial mice with α-lactose treatment in comparison of those from malarial mice. Furthermore, significant positive correlations existed between the mRNA levels of Gal-9 and Tim-3/IFNγ/TREM-1 in both the livers and the peritoneal macrophages, and between Gal-9 and Tim-3/TREM-1 in the spleens of malarial mice; significant positive correlations existed between the mRNA levels of Gal-9 and IFNγ in the livers and between Gal-9 and IFNα in the peritoneal macrophages from malarial mice treated with α-lactose. Our data suggest a potential role of galectin-receptor interactions in limiting liver inflammatory response and parasite proliferation by down-regulating the expressions of IFNα, IFNγ, and TREM-1 during PbANKA infection.Yifan WuShiguang HuangSiyu XiaoJian HeJian HeFangli LuFangli LuFangli LuFrontiers Media S.A.articlePlasmodium bergheilivermacrophageGal-9Tim-3IFNαImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plasmodium berghei liver macrophage Gal-9 Tim-3 IFNα Immunologic diseases. Allergy RC581-607
spellingShingle	Plasmodium berghei liver macrophage Gal-9 Tim-3 IFNα Immunologic diseases. Allergy RC581-607 Yifan Wu Shiguang Huang Siyu Xiao Jian He Jian He Fangli Lu Fangli Lu Fangli Lu Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
description	Hepatopathy is frequently observed in patients with severe malaria but its pathogenesis remains unclear. Galectins are evolutionarily conserved glycan-binding proteins with pleiotropic roles in innate and adaptive immune responses, and exhibit pivotal roles during Plasmodium spp. infection. Here, we analyzed the impact of blockage of galectin-receptor interactions by treatment with alpha (α)-lactose on liver immunopathology during the erythrocytic stage of malaria in mice infected with Plasmodium berghei ANKA (PbANKA). Our results found that compared with PbANKA-infected mice (malarial mice), blockage of galectin-receptor interactions led to decreased host survival rate and increased peripheral blood parasitemia; exacerbated liver pathology, increased numbers of CD68+ macrophages and apoptotic cells, and increased parasite burden in the livers on days 5 and 7 post infection (p.i.) as well as increased mRNA expression levels of galectin-9 (Gal-9) and its receptor, the T cell immunoglobulin domain and mucin domain protein 3 (Tim-3), interferon (IFN)α, IFNγ, and the triggering receptor expressed on myeloid cells (TREM)-1 in the livers or spleens of PbANKA-infected mice on day 7 p.i. Observed by transmission electron microscopy, the peritoneal macrophages isolated from malarial mice with α-lactose treatment had more pseudopodia than those from malarial mice. Measured by using quantitative real-time reverse transcription-polymerase chain reaction assay, the mRNA expression levels of Gal-9, IFNα, IFNβ, IFNγ, and TREM-1 were increased in the peritoneal macrophages isolated from malarial mice with α-lactose treatment in comparison of those from malarial mice. Furthermore, significant positive correlations existed between the mRNA levels of Gal-9 and Tim-3/IFNγ/TREM-1 in both the livers and the peritoneal macrophages, and between Gal-9 and Tim-3/TREM-1 in the spleens of malarial mice; significant positive correlations existed between the mRNA levels of Gal-9 and IFNγ in the livers and between Gal-9 and IFNα in the peritoneal macrophages from malarial mice treated with α-lactose. Our data suggest a potential role of galectin-receptor interactions in limiting liver inflammatory response and parasite proliferation by down-regulating the expressions of IFNα, IFNγ, and TREM-1 during PbANKA infection.
format	article
author	Yifan Wu Shiguang Huang Siyu Xiao Jian He Jian He Fangli Lu Fangli Lu Fangli Lu
author_facet	Yifan Wu Shiguang Huang Siyu Xiao Jian He Jian He Fangli Lu Fangli Lu Fangli Lu
author_sort	Yifan Wu
title	Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
title_short	Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
title_full	Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
title_fullStr	Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
title_full_unstemmed	Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria
title_sort	impact of galectin-receptor interactions on liver pathology during the erythrocytic stage of plasmodium berghei malaria
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/210407235459463b89ee383692d6f326
work_keys_str_mv	AT yifanwu impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT shiguanghuang impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT siyuxiao impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT jianhe impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT jianhe impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT fanglilu impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT fanglilu impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria AT fanglilu impactofgalectinreceptorinteractionsonliverpathologyduringtheerythrocyticstageofplasmodiumbergheimalaria
_version_	1718406309775147008

Impact of Galectin-Receptor Interactions on Liver Pathology During the Erythrocytic Stage of Plasmodium berghei Malaria

Ejemplares similares