Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar
Rickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the p...
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| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/210558681c9b47a59a092b1a47823478 |
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| Sumario: | Rickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the presence of rickettsial DNA. EDTA (ethylenediaminetetraacetic acid) blood from 1020 patients presenting with pyrexia > 38.5 °C was analyzed by <i>gltA</i>-specific qPCR. Positive samples were confirmed by <i>ompB</i>-specific qPCR. From confirmed samples, the <i>gltA</i> amplicons were sequenced and subjected to phylogenetic analysis. From five <i>gltA</i>-reactive samples, two were confirmed by <i>ompB</i>-specific qPCR. The <i>gltA</i> sequence in the sample taken from a 38-year-old female showed 100% homology with <i>R. typhi</i>. The other sample taken from a 1.5-year-old infant was 100% homologous to <i>R. felis</i>. Tick-borne rickettsiae were not identified. The overall rate of febrile patients with molecular evidence for a rickettsial infection from the Madagascan study site was 0.2% (2/1020 patients). Flea-borne rickettsiosis is a rare but neglected cause of infection in Madagascar. Accurate diagnosis may prompt adequate antimicrobial treatment. |
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