Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders

In poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used auto...

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Autores principales:	Sofie Kromann, Rikke Heidemann Olsen, Anders Miki Bojesen, Henrik Elvang Jensen, Ida Thøfner
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	APEC colibacillosis challenge study poultry disease disease prevention bacterin vaccine Medicine R
Acceso en línea:	https://doaj.org/article/210f730ed408473393f04b02cbc746cc
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spelling	oai:doaj.org-article:210f730ed408473393f04b02cbc746cc2021-11-25T19:10:19ZProtective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders10.3390/vaccines91112332076-393Xhttps://doaj.org/article/210f730ed408473393f04b02cbc746cc2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1233https://doaj.org/toc/2076-393XIn poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used autogenous vaccine, previously deemed ineffective, in an aerosol model of colibacillosis. Methods: Broiler breeders (<i>n</i> = 47) were randomly allocated to one of four groups (vaccinated and unvaccinated birds receiving an autogenous vaccine or sterile saline intramuscularly) and challenged with either aerosolised <i>E. coli</i> or vehicle at 29 weeks of age. Two days following inoculation, the birds were euthanised, thoroughly necropsied, and samples for bacteriology and histopathology were collected. Results: Vaccinated birds had a significantly lower bacteriology score compared to the unvaccinated group challenged with <i>E. coli</i> (<i>p</i> < 0.01) and a lower overall air sac lesion score (<i>p</i> < 0.05). Overall lung and spleen lesion scores only differed significantly between the unvaccinated <i>E. coli</i> challenged group compared to the vehicle inoculated groups. The overall gross pathology score was 2.8 and 1.95 in the unvaccinated and vaccinated <i>E. coli</i> challenge groups, respectively, whereas the vaccinated vehicle group had a score of 0.9 and the unvaccinated vehicle group a score of 1. Conclusions: A protective effect of an autogenous vaccine was found utilising an aerogenous model of colibacillosis through multiple methods of evaluation. The findings encourage the continued use of autogenous vaccines and underlines the necessity of discriminative experimental models with high predictive validity when evaluating vaccine interventions.Sofie KromannRikke Heidemann OlsenAnders Miki BojesenHenrik Elvang JensenIda ThøfnerMDPI AGarticleAPECcolibacillosischallenge studypoultry diseasedisease preventionbacterin vaccineMedicineRENVaccines, Vol 9, Iss 1233, p 1233 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	APEC colibacillosis challenge study poultry disease disease prevention bacterin vaccine Medicine R
spellingShingle	APEC colibacillosis challenge study poultry disease disease prevention bacterin vaccine Medicine R Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
description	In poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used autogenous vaccine, previously deemed ineffective, in an aerosol model of colibacillosis. Methods: Broiler breeders (<i>n</i> = 47) were randomly allocated to one of four groups (vaccinated and unvaccinated birds receiving an autogenous vaccine or sterile saline intramuscularly) and challenged with either aerosolised <i>E. coli</i> or vehicle at 29 weeks of age. Two days following inoculation, the birds were euthanised, thoroughly necropsied, and samples for bacteriology and histopathology were collected. Results: Vaccinated birds had a significantly lower bacteriology score compared to the unvaccinated group challenged with <i>E. coli</i> (<i>p</i> < 0.01) and a lower overall air sac lesion score (<i>p</i> < 0.05). Overall lung and spleen lesion scores only differed significantly between the unvaccinated <i>E. coli</i> challenged group compared to the vehicle inoculated groups. The overall gross pathology score was 2.8 and 1.95 in the unvaccinated and vaccinated <i>E. coli</i> challenge groups, respectively, whereas the vaccinated vehicle group had a score of 0.9 and the unvaccinated vehicle group a score of 1. Conclusions: A protective effect of an autogenous vaccine was found utilising an aerogenous model of colibacillosis through multiple methods of evaluation. The findings encourage the continued use of autogenous vaccines and underlines the necessity of discriminative experimental models with high predictive validity when evaluating vaccine interventions.
format	article
author	Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner
author_facet	Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner
author_sort	Sofie Kromann
title	Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
title_short	Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
title_full	Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
title_fullStr	Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
title_full_unstemmed	Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
title_sort	protective potential of an autogenous vaccine in an aerogenous model of <i>escherichia coli</i> infection in broiler breeders
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/210f730ed408473393f04b02cbc746cc
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Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders

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