Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample

<h4>Background</h4> Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of...

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Autores principales:	Amanda Caplan, Kelly W. Bates, Carla Brioni, Aileen Santos, Linda M. Sabatini, Karen L. Kaul, Mercedes R. Carnethon, Janardan D. Khandekar, Philip Greenland
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2115abcf2f5240b3980b069fe2b627eb
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spelling	oai:doaj.org-article:2115abcf2f5240b3980b069fe2b627eb2021-11-04T06:07:15ZClinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample1932-6203https://doaj.org/article/2115abcf2f5240b3980b069fe2b627eb2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530348/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4> Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. <h4>Methods</h4> This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3–5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. <h4>Results</h4> Twenty-five patients (nine females) were enrolled, ranging in age from 19–58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6–67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration (β = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity. <h4>Conclusions</h4> In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.Amanda CaplanKelly W. BatesCarla BrioniAileen SantosLinda M. SabatiniKaren L. KaulMercedes R. CarnethonJanardan D. KhandekarPhilip GreenlandPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amanda Caplan Kelly W. Bates Carla Brioni Aileen Santos Linda M. Sabatini Karen L. Kaul Mercedes R. Carnethon Janardan D. Khandekar Philip Greenland Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
description	<h4>Background</h4> Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. <h4>Methods</h4> This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3–5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. <h4>Results</h4> Twenty-five patients (nine females) were enrolled, ranging in age from 19–58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6–67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration (β = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity. <h4>Conclusions</h4> In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.
format	article
author	Amanda Caplan Kelly W. Bates Carla Brioni Aileen Santos Linda M. Sabatini Karen L. Kaul Mercedes R. Carnethon Janardan D. Khandekar Philip Greenland
author_facet	Amanda Caplan Kelly W. Bates Carla Brioni Aileen Santos Linda M. Sabatini Karen L. Kaul Mercedes R. Carnethon Janardan D. Khandekar Philip Greenland
author_sort	Amanda Caplan
title	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
title_short	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
title_full	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
title_fullStr	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
title_full_unstemmed	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
title_sort	clinical characteristics and viral load dynamics of covid-19 in a mildly or moderately symptomatic outpatient sample
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2115abcf2f5240b3980b069fe2b627eb
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Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample

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