Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Given the rise of morbidity and mortality caused by Klebsiella pneumoniae (KP), the increasing number of strains resistant to antibiotics, and the emergence of hypervirulent Klebsiella pneumonia, treatment of KP infection becomes difficult; thus, novel drugs are...

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Autores principales:	Guokai Dong, Nana Xu, Meng Wang, Yunyun Zhao, Fei Jiang, Huimin Bu, Jinjuan Liu, Bo Yuan, Rongpeng Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Klebsiella pneumoniae</i> purple sweet potato anthocyanins PSPAs pyroptosis mitophagy NLRP3 inflammasome Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2117ea633f3446489669ea6faf0a16c1
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spelling	oai:doaj.org-article:2117ea633f3446489669ea6faf0a16c12021-11-11T16:53:35ZAnthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection10.3390/ijms2221114221422-00671661-6596https://doaj.org/article/2117ea633f3446489669ea6faf0a16c12021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11422https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Given the rise of morbidity and mortality caused by <i>Klebsiella pneumoniae</i> (KP), the increasing number of strains resistant to antibiotics, and the emergence of <i>hypervirulent Klebsiella pneumonia</i>, treatment of KP infection becomes difficult; thus, novel drugs are necessary for treatment. Anthocyanins, or natural flavonoids, have an extensive effect against bacterial infection. However, few studies on anti-KP are identified. Here, we evaluated the therapeutic effect of purple sweet potato anthocyanins (PSPAs) on KP, containing 98.7% delphinidin 3-sambubioside. Results showed that KP-infected mice after PSPAs treatment manifested decreased mortality, weakened lung injury, dampened inflammatory responses, and reduced bacterial systemic dissemination in vivo. In Vitro, PSPAs significantly suppressed pyroptosis and restricted NLRP3 inflammasome activation in alveolar macrophages infected with KP. As for the mechanism, PSPAs promote mitophagy by recruiting Parkin to the mitochondria. PSPAs-conferred mitophagy increased mitochondrial membrane potential and decreased mitochondrial reactive oxygen species and mitochondrial DNA, resulting in impaired NLRP3 inflammasome activation. In addition, the promotion of mitophagy by PSPAs required the Nrf2 signaling pathway. Collectively, these findings suggest that PSPAs are a potential option for the treatment of KP infection.Guokai DongNana XuMeng WangYunyun ZhaoFei JiangHuimin BuJinjuan LiuBo YuanRongpeng LiMDPI AGarticle<i>Klebsiella pneumoniae</i>purple sweet potato anthocyanins PSPAspyroptosismitophagyNLRP3 inflammasomeBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11422, p 11422 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Klebsiella pneumoniae</i> purple sweet potato anthocyanins PSPAs pyroptosis mitophagy NLRP3 inflammasome Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Klebsiella pneumoniae</i> purple sweet potato anthocyanins PSPAs pyroptosis mitophagy NLRP3 inflammasome Biology (General) QH301-705.5 Chemistry QD1-999 Guokai Dong Nana Xu Meng Wang Yunyun Zhao Fei Jiang Huimin Bu Jinjuan Liu Bo Yuan Rongpeng Li Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
description	Given the rise of morbidity and mortality caused by <i>Klebsiella pneumoniae</i> (KP), the increasing number of strains resistant to antibiotics, and the emergence of <i>hypervirulent Klebsiella pneumonia</i>, treatment of KP infection becomes difficult; thus, novel drugs are necessary for treatment. Anthocyanins, or natural flavonoids, have an extensive effect against bacterial infection. However, few studies on anti-KP are identified. Here, we evaluated the therapeutic effect of purple sweet potato anthocyanins (PSPAs) on KP, containing 98.7% delphinidin 3-sambubioside. Results showed that KP-infected mice after PSPAs treatment manifested decreased mortality, weakened lung injury, dampened inflammatory responses, and reduced bacterial systemic dissemination in vivo. In Vitro, PSPAs significantly suppressed pyroptosis and restricted NLRP3 inflammasome activation in alveolar macrophages infected with KP. As for the mechanism, PSPAs promote mitophagy by recruiting Parkin to the mitochondria. PSPAs-conferred mitophagy increased mitochondrial membrane potential and decreased mitochondrial reactive oxygen species and mitochondrial DNA, resulting in impaired NLRP3 inflammasome activation. In addition, the promotion of mitophagy by PSPAs required the Nrf2 signaling pathway. Collectively, these findings suggest that PSPAs are a potential option for the treatment of KP infection.
format	article
author	Guokai Dong Nana Xu Meng Wang Yunyun Zhao Fei Jiang Huimin Bu Jinjuan Liu Bo Yuan Rongpeng Li
author_facet	Guokai Dong Nana Xu Meng Wang Yunyun Zhao Fei Jiang Huimin Bu Jinjuan Liu Bo Yuan Rongpeng Li
author_sort	Guokai Dong
title	Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
title_short	Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
title_full	Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
title_fullStr	Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
title_full_unstemmed	Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection
title_sort	anthocyanin extract from purple sweet potato exacerbate mitophagy to ameliorate pyroptosis in <i>klebsiella pneumoniae</i> infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2117ea633f3446489669ea6faf0a16c1
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Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in <i>Klebsiella pneumoniae</i> Infection

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