Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.

Membranous Nephropathy (MN) represents a large amount of Nephrotic Syndromes in the adult population and its definitive diagnosis is currently carried out through biopsy. An autoimmune condition has been demonstrated in idiopathic MN (iMN) in which some kidney structures are targeted by patient auto...

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Autores principales:	Fabrizio Cavazzini, Riccardo Magistroni, Luciana Furci, Valentina Lupo, Giulia Ligabue, Maria Granito, Marco Leonelli, Alberto Albertazzi, Gianni Cappelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2124f04fd0c34ee7ba14429933503eed
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spelling	oai:doaj.org-article:2124f04fd0c34ee7ba14429933503eed2021-11-18T08:09:25ZIdentification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.1932-620310.1371/journal.pone.0048845https://doaj.org/article/2124f04fd0c34ee7ba14429933503eed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144993/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Membranous Nephropathy (MN) represents a large amount of Nephrotic Syndromes in the adult population and its definitive diagnosis is currently carried out through biopsy. An autoimmune condition has been demonstrated in idiopathic MN (iMN) in which some kidney structures are targeted by patient autoantibodies. Some candidate antigens have been described and other likely involved target proteins responsible for the disease are not known yet. In this work our aim is to identify these proteins by screening a lambda-phage library with patients' sera. We enrolled four groups of patients: two MN groups of 12 full iMN patients; one control group of 15 patients suffering from other renal diseases; one control group of 15 healthy individuals. A commercial cDNA phagemide library was screened using the above described sera, in order to detect positive signals due to antigen-antibody bond. We detected one phagemide clone expressing a protein which was shown to be targeted by the antibodies of the iMN sera only. Control sera were negative. The sequence analysis of cDNA matched the Synaptonemal Complex protein 65 (SC65) coding sequence. Further proteomic analyses were carried out to validate our results. We provide evidence of an involvement of SC65 protein as an autoimmune target in iMN. Considering the invasiveness and the resulting risk coming from renal biopsy, our ongoing aim is to set a procedure able to diagnose affected patients through a little- or non-invasive method such as blood sampling rather than biopsy.Fabrizio CavazziniRiccardo MagistroniLuciana FurciValentina LupoGiulia LigabueMaria GranitoMarco LeonelliAlberto AlbertazziGianni CappelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48845 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabrizio Cavazzini Riccardo Magistroni Luciana Furci Valentina Lupo Giulia Ligabue Maria Granito Marco Leonelli Alberto Albertazzi Gianni Cappelli Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
description	Membranous Nephropathy (MN) represents a large amount of Nephrotic Syndromes in the adult population and its definitive diagnosis is currently carried out through biopsy. An autoimmune condition has been demonstrated in idiopathic MN (iMN) in which some kidney structures are targeted by patient autoantibodies. Some candidate antigens have been described and other likely involved target proteins responsible for the disease are not known yet. In this work our aim is to identify these proteins by screening a lambda-phage library with patients' sera. We enrolled four groups of patients: two MN groups of 12 full iMN patients; one control group of 15 patients suffering from other renal diseases; one control group of 15 healthy individuals. A commercial cDNA phagemide library was screened using the above described sera, in order to detect positive signals due to antigen-antibody bond. We detected one phagemide clone expressing a protein which was shown to be targeted by the antibodies of the iMN sera only. Control sera were negative. The sequence analysis of cDNA matched the Synaptonemal Complex protein 65 (SC65) coding sequence. Further proteomic analyses were carried out to validate our results. We provide evidence of an involvement of SC65 protein as an autoimmune target in iMN. Considering the invasiveness and the resulting risk coming from renal biopsy, our ongoing aim is to set a procedure able to diagnose affected patients through a little- or non-invasive method such as blood sampling rather than biopsy.
format	article
author	Fabrizio Cavazzini Riccardo Magistroni Luciana Furci Valentina Lupo Giulia Ligabue Maria Granito Marco Leonelli Alberto Albertazzi Gianni Cappelli
author_facet	Fabrizio Cavazzini Riccardo Magistroni Luciana Furci Valentina Lupo Giulia Ligabue Maria Granito Marco Leonelli Alberto Albertazzi Gianni Cappelli
author_sort	Fabrizio Cavazzini
title	Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
title_short	Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
title_full	Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
title_fullStr	Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
title_full_unstemmed	Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
title_sort	identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cdna library.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2124f04fd0c34ee7ba14429933503eed
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Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.

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