Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan
Abstract Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), fo...
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Nature Portfolio
2017
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oai:doaj.org-article:2125c600018344408bb8db25560f10032021-12-02T11:40:30ZOblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan10.1038/s41598-017-08822-02045-2322https://doaj.org/article/2125c600018344408bb8db25560f10032017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08822-0https://doaj.org/toc/2045-2322Abstract Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth. Here, the use of an obliquely scanning laser eliminates the z-stacking process, then allows highly efficient 3D OCT and fluorescence imaging by using only one raster scan. The current setup provides ~3.6 × 4.2 × 6.5 μm resolution in fluorescence imaging, ~7 × 7 × 3.5 μm in OCT in three dimensions, and the current speed of imaging is up to 100 frames per second (fps) over a volume about 0.8 × 1 × 0.5 mm3. We demonstrate several mechanisms for molecular imaging, including intrinsically expressed GFP fluorescence, autofluorescence from Flavin proteins, and exogenous antibody-conjugated dyes. We also demonstrate potential applications in imaging human intestinal organoids (HIOs), colon mucosa, and retina.Lei ZhangAmalia CapillaWeiye SongGustavo MostoslavskyJi YiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Lei Zhang Amalia Capilla Weiye Song Gustavo Mostoslavsky Ji Yi Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| description |
Abstract Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth. Here, the use of an obliquely scanning laser eliminates the z-stacking process, then allows highly efficient 3D OCT and fluorescence imaging by using only one raster scan. The current setup provides ~3.6 × 4.2 × 6.5 μm resolution in fluorescence imaging, ~7 × 7 × 3.5 μm in OCT in three dimensions, and the current speed of imaging is up to 100 frames per second (fps) over a volume about 0.8 × 1 × 0.5 mm3. We demonstrate several mechanisms for molecular imaging, including intrinsically expressed GFP fluorescence, autofluorescence from Flavin proteins, and exogenous antibody-conjugated dyes. We also demonstrate potential applications in imaging human intestinal organoids (HIOs), colon mucosa, and retina. |
| format |
article |
| author |
Lei Zhang Amalia Capilla Weiye Song Gustavo Mostoslavsky Ji Yi |
| author_facet |
Lei Zhang Amalia Capilla Weiye Song Gustavo Mostoslavsky Ji Yi |
| author_sort |
Lei Zhang |
| title |
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| title_short |
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| title_full |
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| title_fullStr |
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| title_full_unstemmed |
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| title_sort |
oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/2125c600018344408bb8db25560f1003 |
| work_keys_str_mv |
AT leizhang obliquescanninglasermicroscopyforsimultaneouslyvolumetricstructuralandmolecularimagingusingonlyonerasterscan AT amaliacapilla obliquescanninglasermicroscopyforsimultaneouslyvolumetricstructuralandmolecularimagingusingonlyonerasterscan AT weiyesong obliquescanninglasermicroscopyforsimultaneouslyvolumetricstructuralandmolecularimagingusingonlyonerasterscan AT gustavomostoslavsky obliquescanninglasermicroscopyforsimultaneouslyvolumetricstructuralandmolecularimagingusingonlyonerasterscan AT jiyi obliquescanninglasermicroscopyforsimultaneouslyvolumetricstructuralandmolecularimagingusingonlyonerasterscan |
| _version_ |
1718395568349249536 |