The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients

Hepatitis C Virus (HCV) infection is frequently diagnosed by detection of antibodyagainst the HCV (Anti-HCV). The seropositivity of anti-HCV could reflect chronicinfectious status and/or previous infection. Detection of HCV-RNA by PCR is stilllaborious, too expensive, requires specific expertise and...

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Autores principales:	Tuğrul Himmetoğlu, Hüseyin Şimşek, Aygül Türkmen, Doğan Cengiz, Gülsevim Saydam, Mevhibe Balk
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2007
Materias:	Anti- HCV HCV-RNA ALT Hepatitis C virus infection Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/21286594a1d44fe69707d58981b90913
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spelling	oai:doaj.org-article:21286594a1d44fe69707d58981b909132021-12-02T14:57:03ZThe Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients0250-46851303-829Xhttps://doaj.org/article/21286594a1d44fe69707d58981b909132007-07-01T00:00:00Zhttp://www.TurkJBiochem.com/2007/051.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XHepatitis C Virus (HCV) infection is frequently diagnosed by detection of antibodyagainst the HCV (Anti-HCV). The seropositivity of anti-HCV could reflect chronicinfectious status and/or previous infection. Detection of HCV-RNA by PCR is stilllaborious, too expensive, requires specific expertise and facilities, and usually used toconfirm positive serology. This study was performed in anti-HCV positive patients todetermine the relationship between anti-HCV Sample rate / Cutoff rate (S/CO) ratios,HCV-RNA and Alanine aminotransferase (ALT) levels. In addition, the utility of anti-HCV S/CO ratio values in predicting HCV viremia in anti-HCV positive patients wereevaluated. Serum samples of 124 patients were tested for anti-HCV by a MEIA technique,for HCV-RNA by a quantitative PCR and for ALT by IFCC UV test. S/CO values foranti-HCV test and ALT levels were correlated with the quantitative values of HCV-RNA(respectively, r=0.824, p<0.0001 and r=0.795, p<0.0001). HCV-RNA values were foundto be negative in samples with S/CO ≤ 25.9. HCV-RNA positivity was found in 100 %of samples with a S/CO > 25.9. All HCV-RNA negative cases had normal serum ALTlevels (24.67 ± 8.56 U/L) and relatively low S/CO values (3.81 ± 4.32) for anti-HCV tests.HCV-RNA positive other cases had greater S/CO values (119.53 ± 37.99) and elevatedserum ALT levels (99.53 ± 49.96 U/L). The sensitivity and specificity of anti-HCV in thediagnosis of HCV viremia was 100 % and specificity was 100 % respectively when S/CO ratio was set 25.9 as a cutoff. Among patients with high S/CO ratios, the likelihoodof HCV-RNA positivity was directly related to S/CO ratio. S/CO values may be used topredict HCV viremia in Anti-HCV positive individuals and therefore, quantitative HCVRNAtesting could not be routinely required for all patients.Tuğrul HimmetoğluHüseyin ŞimşekAygül TürkmenDoğan CengizGülsevim SaydamMevhibe BalkDe GruyterarticleAnti- HCVHCV-RNAALTHepatitis C virus infectionBiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 32, Iss 2, Pp 51-54 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anti- HCV HCV-RNA ALT Hepatitis C virus infection Biochemistry QD415-436
spellingShingle	Anti- HCV HCV-RNA ALT Hepatitis C virus infection Biochemistry QD415-436 Tuğrul Himmetoğlu Hüseyin Şimşek Aygül Türkmen Doğan Cengiz Gülsevim Saydam Mevhibe Balk The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
description	Hepatitis C Virus (HCV) infection is frequently diagnosed by detection of antibodyagainst the HCV (Anti-HCV). The seropositivity of anti-HCV could reflect chronicinfectious status and/or previous infection. Detection of HCV-RNA by PCR is stilllaborious, too expensive, requires specific expertise and facilities, and usually used toconfirm positive serology. This study was performed in anti-HCV positive patients todetermine the relationship between anti-HCV Sample rate / Cutoff rate (S/CO) ratios,HCV-RNA and Alanine aminotransferase (ALT) levels. In addition, the utility of anti-HCV S/CO ratio values in predicting HCV viremia in anti-HCV positive patients wereevaluated. Serum samples of 124 patients were tested for anti-HCV by a MEIA technique,for HCV-RNA by a quantitative PCR and for ALT by IFCC UV test. S/CO values foranti-HCV test and ALT levels were correlated with the quantitative values of HCV-RNA(respectively, r=0.824, p<0.0001 and r=0.795, p<0.0001). HCV-RNA values were foundto be negative in samples with S/CO ≤ 25.9. HCV-RNA positivity was found in 100 %of samples with a S/CO > 25.9. All HCV-RNA negative cases had normal serum ALTlevels (24.67 ± 8.56 U/L) and relatively low S/CO values (3.81 ± 4.32) for anti-HCV tests.HCV-RNA positive other cases had greater S/CO values (119.53 ± 37.99) and elevatedserum ALT levels (99.53 ± 49.96 U/L). The sensitivity and specificity of anti-HCV in thediagnosis of HCV viremia was 100 % and specificity was 100 % respectively when S/CO ratio was set 25.9 as a cutoff. Among patients with high S/CO ratios, the likelihoodof HCV-RNA positivity was directly related to S/CO ratio. S/CO values may be used topredict HCV viremia in Anti-HCV positive individuals and therefore, quantitative HCVRNAtesting could not be routinely required for all patients.
format	article
author	Tuğrul Himmetoğlu Hüseyin Şimşek Aygül Türkmen Doğan Cengiz Gülsevim Saydam Mevhibe Balk
author_facet	Tuğrul Himmetoğlu Hüseyin Şimşek Aygül Türkmen Doğan Cengiz Gülsevim Saydam Mevhibe Balk
author_sort	Tuğrul Himmetoğlu
title	The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
title_short	The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
title_full	The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
title_fullStr	The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
title_full_unstemmed	The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients
title_sort	utility of anti-hcv s/co ratio, hcv-rna and alt test in predicting viremia in anti-hcv positive patients
publisher	De Gruyter
publishDate	2007
url	https://doaj.org/article/21286594a1d44fe69707d58981b90913
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The Utility of ANTI-HCV S/CO Ratio, HCV-RNA and ALT Test in Predicting Viremia in ANTI-HCV Positive Patients

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