Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay

ABSTRACT Polyomaviruses (PyVs) are known to infect a wide range of vertebrates and invertebrates and are associated with a broad spectrum of diseases, including cancers, particularly in immune-suppressed hosts. A novel polyomavirus, designated rat polyomavirus 2 (RatPyV2), was identified from a bree...

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Autores principales:	Lora H. Rigatti, Tuna Toptan, Joseph T. Newsome, Patrick S. Moore, Yuan Chang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	epitheliotropism immune suppression KIV P-PIT WUV X-SCID Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/212d01508ed84c5d810ff76ee7ca8d4e
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spelling	oai:doaj.org-article:212d01508ed84c5d810ff76ee7ca8d4e2021-11-15T15:22:04ZIdentification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay10.1128/mSphere.00334-162379-5042https://doaj.org/article/212d01508ed84c5d810ff76ee7ca8d4e2016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00334-16https://doaj.org/toc/2379-5042ABSTRACT Polyomaviruses (PyVs) are known to infect a wide range of vertebrates and invertebrates and are associated with a broad spectrum of diseases, including cancers, particularly in immune-suppressed hosts. A novel polyomavirus, designated rat polyomavirus 2 (RatPyV2), was identified from a breeding colony of rats having X-linked severe combined immunodeficiency. Using a human panpolyomavirus immunohistochemistry test (P-PIT), RatPyV2 was initially detected in the parotid salivary gland of a colony member. Rolling circle amplification using DNA from harderian and parotid glands identified a novel 5.1-kb polyomavirus genome closely related to human Washington University (WU) and Karolinska Institute (KI) and vole polyomaviruses but notably divergent from Rattus norvegicus PyV1 (RnorPyV1; also designated RatPyV1). Further screening showed RatPyV2 inclusion body infection in the lung epithelium and variably in other respiratory, reproductive, and glandular tissues of 12/12 (100%) rats. IMPORTANCE Although P-PIT was developed to detect diseases associated with known human polyomaviruses, the identification of a new polyomavirus in rats suggests that it may have utility as a broad-based screen for new, as well as known polyomaviruses. Our findings suggest that RatPyV2 may be a commensal infection of laboratory rats that can lead to disseminated disease in T cell immune-deficient rats. Infection of the X-SCID rats with RatPyV2 and Pneumocystis carinii is a potential model for coinfection pathogenesis and treatment options during transplant preclinical studies.Lora H. RigattiTuna ToptanJoseph T. NewsomePatrick S. MooreYuan ChangAmerican Society for Microbiologyarticleepitheliotropismimmune suppressionKIVP-PITWUVX-SCIDMicrobiologyQR1-502ENmSphere, Vol 1, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	epitheliotropism immune suppression KIV P-PIT WUV X-SCID Microbiology QR1-502
spellingShingle	epitheliotropism immune suppression KIV P-PIT WUV X-SCID Microbiology QR1-502 Lora H. Rigatti Tuna Toptan Joseph T. Newsome Patrick S. Moore Yuan Chang Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
description	ABSTRACT Polyomaviruses (PyVs) are known to infect a wide range of vertebrates and invertebrates and are associated with a broad spectrum of diseases, including cancers, particularly in immune-suppressed hosts. A novel polyomavirus, designated rat polyomavirus 2 (RatPyV2), was identified from a breeding colony of rats having X-linked severe combined immunodeficiency. Using a human panpolyomavirus immunohistochemistry test (P-PIT), RatPyV2 was initially detected in the parotid salivary gland of a colony member. Rolling circle amplification using DNA from harderian and parotid glands identified a novel 5.1-kb polyomavirus genome closely related to human Washington University (WU) and Karolinska Institute (KI) and vole polyomaviruses but notably divergent from Rattus norvegicus PyV1 (RnorPyV1; also designated RatPyV1). Further screening showed RatPyV2 inclusion body infection in the lung epithelium and variably in other respiratory, reproductive, and glandular tissues of 12/12 (100%) rats. IMPORTANCE Although P-PIT was developed to detect diseases associated with known human polyomaviruses, the identification of a new polyomavirus in rats suggests that it may have utility as a broad-based screen for new, as well as known polyomaviruses. Our findings suggest that RatPyV2 may be a commensal infection of laboratory rats that can lead to disseminated disease in T cell immune-deficient rats. Infection of the X-SCID rats with RatPyV2 and Pneumocystis carinii is a potential model for coinfection pathogenesis and treatment options during transplant preclinical studies.
format	article
author	Lora H. Rigatti Tuna Toptan Joseph T. Newsome Patrick S. Moore Yuan Chang
author_facet	Lora H. Rigatti Tuna Toptan Joseph T. Newsome Patrick S. Moore Yuan Chang
author_sort	Lora H. Rigatti
title	Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
title_short	Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
title_full	Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
title_fullStr	Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
title_full_unstemmed	Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay
title_sort	identification and characterization of novel rat polyomavirus 2 in a colony of x-scid rats by p-pit assay
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/212d01508ed84c5d810ff76ee7ca8d4e
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Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay

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