Characterization and reverse genetic establishment of cattle derived Akabane virus in China

Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016....

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Autores principales: Dongjie Chen, Di Wang, Fang Wei, Yufang Kong, Junhua Deng, Xiangmei Lin, Shaoqiang Wu
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Lenguaje:EN
Publicado: BMC 2021
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Acceso en línea:https://doaj.org/article/212f26954add407faecfe8592580899c
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spelling oai:doaj.org-article:212f26954add407faecfe8592580899c2021-11-21T12:26:00ZCharacterization and reverse genetic establishment of cattle derived Akabane virus in China10.1186/s12917-021-03054-x1746-6148https://doaj.org/article/212f26954add407faecfe8592580899c2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03054-xhttps://doaj.org/toc/1746-6148Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.Dongjie ChenDi WangFang WeiYufang KongJunhua DengXiangmei LinShaoqiang WuBMCarticleAkabane virusIsolatePhylogenetic analysisReverse genetic systemVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Akabane virus
Isolate
Phylogenetic analysis
Reverse genetic system
Veterinary medicine
SF600-1100
spellingShingle Akabane virus
Isolate
Phylogenetic analysis
Reverse genetic system
Veterinary medicine
SF600-1100
Dongjie Chen
Di Wang
Fang Wei
Yufang Kong
Junhua Deng
Xiangmei Lin
Shaoqiang Wu
Characterization and reverse genetic establishment of cattle derived Akabane virus in China
description Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.
format article
author Dongjie Chen
Di Wang
Fang Wei
Yufang Kong
Junhua Deng
Xiangmei Lin
Shaoqiang Wu
author_facet Dongjie Chen
Di Wang
Fang Wei
Yufang Kong
Junhua Deng
Xiangmei Lin
Shaoqiang Wu
author_sort Dongjie Chen
title Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_short Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_full Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_fullStr Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_full_unstemmed Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_sort characterization and reverse genetic establishment of cattle derived akabane virus in china
publisher BMC
publishDate 2021
url https://doaj.org/article/212f26954add407faecfe8592580899c
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