Characterization and reverse genetic establishment of cattle derived Akabane virus in China
Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016....
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oai:doaj.org-article:212f26954add407faecfe8592580899c2021-11-21T12:26:00ZCharacterization and reverse genetic establishment of cattle derived Akabane virus in China10.1186/s12917-021-03054-x1746-6148https://doaj.org/article/212f26954add407faecfe8592580899c2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03054-xhttps://doaj.org/toc/1746-6148Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.Dongjie ChenDi WangFang WeiYufang KongJunhua DengXiangmei LinShaoqiang WuBMCarticleAkabane virusIsolatePhylogenetic analysisReverse genetic systemVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021) |
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Akabane virus Isolate Phylogenetic analysis Reverse genetic system Veterinary medicine SF600-1100 |
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Akabane virus Isolate Phylogenetic analysis Reverse genetic system Veterinary medicine SF600-1100 Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
description |
Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies. |
format |
article |
author |
Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu |
author_facet |
Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu |
author_sort |
Dongjie Chen |
title |
Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
title_short |
Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
title_full |
Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
title_fullStr |
Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
title_full_unstemmed |
Characterization and reverse genetic establishment of cattle derived Akabane virus in China |
title_sort |
characterization and reverse genetic establishment of cattle derived akabane virus in china |
publisher |
BMC |
publishDate |
2021 |
url |
https://doaj.org/article/212f26954add407faecfe8592580899c |
work_keys_str_mv |
AT dongjiechen characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT diwang characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT fangwei characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT yufangkong characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT junhuadeng characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT xiangmeilin characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT shaoqiangwu characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina |
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1718418992566108160 |