Characterization and reverse genetic establishment of cattle derived Akabane virus in China

Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dongjie Chen, Di Wang, Fang Wei, Yufang Kong, Junhua Deng, Xiangmei Lin, Shaoqiang Wu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Akabane virus Isolate Phylogenetic analysis Reverse genetic system Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/212f26954add407faecfe8592580899c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:212f26954add407faecfe8592580899c
record_format	dspace
spelling	oai:doaj.org-article:212f26954add407faecfe8592580899c2021-11-21T12:26:00ZCharacterization and reverse genetic establishment of cattle derived Akabane virus in China10.1186/s12917-021-03054-x1746-6148https://doaj.org/article/212f26954add407faecfe8592580899c2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03054-xhttps://doaj.org/toc/1746-6148Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.Dongjie ChenDi WangFang WeiYufang KongJunhua DengXiangmei LinShaoqiang WuBMCarticleAkabane virusIsolatePhylogenetic analysisReverse genetic systemVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Akabane virus Isolate Phylogenetic analysis Reverse genetic system Veterinary medicine SF600-1100
spellingShingle	Akabane virus Isolate Phylogenetic analysis Reverse genetic system Veterinary medicine SF600-1100 Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu Characterization and reverse genetic establishment of cattle derived Akabane virus in China
description	Abstract Background Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. Results An AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells. Conclusion We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.
format	article
author	Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu
author_facet	Dongjie Chen Di Wang Fang Wei Yufang Kong Junhua Deng Xiangmei Lin Shaoqiang Wu
author_sort	Dongjie Chen
title	Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_short	Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_full	Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_fullStr	Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_full_unstemmed	Characterization and reverse genetic establishment of cattle derived Akabane virus in China
title_sort	characterization and reverse genetic establishment of cattle derived akabane virus in china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/212f26954add407faecfe8592580899c
work_keys_str_mv	AT dongjiechen characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT diwang characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT fangwei characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT yufangkong characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT junhuadeng characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT xiangmeilin characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina AT shaoqiangwu characterizationandreversegeneticestablishmentofcattlederivedakabanevirusinchina
_version_	1718418992566108160

Characterization and reverse genetic establishment of cattle derived Akabane virus in China

Ejemplares similares