Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD
Accelerated ageing is implicated in the pathogenesis of respiratory diseases as chronic obstructive pulmonary disease (COPD), but recent evidence indicates that the COPD can have roots early in life. Here we hypothesise that the accelerated ageing markers might have a role in the pathobiology of you...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:21328096c5dc4b70aabf06761fa0ba5a2021-11-30T18:13:46ZTelomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD2296-858X10.3389/fmed.2021.761767https://doaj.org/article/21328096c5dc4b70aabf06761fa0ba5a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.761767/fullhttps://doaj.org/toc/2296-858XAccelerated ageing is implicated in the pathogenesis of respiratory diseases as chronic obstructive pulmonary disease (COPD), but recent evidence indicates that the COPD can have roots early in life. Here we hypothesise that the accelerated ageing markers might have a role in the pathobiology of young COPD. The objective of this study was to compare two hallmarks of ageing, telomere length (TL), and mitochondrial DNA copy number (mtDNA-CN, as a surrogate marker of mitochondrial dysfunction) in young (≤ 50 years) and old (>50 years) smokers, with and without COPD. Both, TL and mtDNA-CN were measured in whole blood DNA by quantitative PCR [qPCR] in: (1) young ever smokers with (n = 81) or without (n = 166) COPD; and (2) old ever smokers with (n = 159) or without (n = 29) COPD. A multivariable linear regression was used to assess the association of TL and mtDNA-CN with lung function. We observed that in the entire study population, TL and mtDNA-CN decreased with age, and the former but not the latter related to FEV1/FVC (%), FEV1 (% ref.), and DLCO (% ref.). The short telomeres were found both in the young and old patients with severe COPD (FEV1 <50% ref.). In addition, we found that TL and mtDNA-CN were significantly correlated, but their relationship was positive in younger while negative in the older patients with COPD, suggesting a mitochondrial dysfunction. We conclude that TL, but not mtDNA-CN, is associated with the lung function impairment. Both young and old patients with severe COPD have evidence of accelerated ageing (shorter TL) but differ in the direction of the correlation between TL and mtDNA-CN in relation to age.Sandra Casas-RecasensNuria MendozaAlejandra López-GiraldoAlejandra López-GiraldoAlejandra López-GiraldoTamara GarciaBorja G. CosioBorja G. CosioBorja G. CosioSergi Pascual-GuardiaSergi Pascual-GuardiaSergi Pascual-GuardiaAdy Acosta-CastroAdy Acosta-CastroAlicia Borras-SantosAlicia Borras-SantosJoaquim GeaJoaquim GeaJoaquim GeaGloria GarrabouGloria GarrabouGloria GarrabouGloria GarrabouAlvar AgustiAlvar AgustiAlvar AgustiAlvar AgustiRosa FanerRosa FanerFrontiers Media S.A.articletelomeresmitochondrial DNAchronic bronchitisemphysemaageingMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021) |
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telomeres mitochondrial DNA chronic bronchitis emphysema ageing Medicine (General) R5-920 |
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telomeres mitochondrial DNA chronic bronchitis emphysema ageing Medicine (General) R5-920 Sandra Casas-Recasens Nuria Mendoza Alejandra López-Giraldo Alejandra López-Giraldo Alejandra López-Giraldo Tamara Garcia Borja G. Cosio Borja G. Cosio Borja G. Cosio Sergi Pascual-Guardia Sergi Pascual-Guardia Sergi Pascual-Guardia Ady Acosta-Castro Ady Acosta-Castro Alicia Borras-Santos Alicia Borras-Santos Joaquim Gea Joaquim Gea Joaquim Gea Gloria Garrabou Gloria Garrabou Gloria Garrabou Gloria Garrabou Alvar Agusti Alvar Agusti Alvar Agusti Alvar Agusti Rosa Faner Rosa Faner Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| description |
Accelerated ageing is implicated in the pathogenesis of respiratory diseases as chronic obstructive pulmonary disease (COPD), but recent evidence indicates that the COPD can have roots early in life. Here we hypothesise that the accelerated ageing markers might have a role in the pathobiology of young COPD. The objective of this study was to compare two hallmarks of ageing, telomere length (TL), and mitochondrial DNA copy number (mtDNA-CN, as a surrogate marker of mitochondrial dysfunction) in young (≤ 50 years) and old (>50 years) smokers, with and without COPD. Both, TL and mtDNA-CN were measured in whole blood DNA by quantitative PCR [qPCR] in: (1) young ever smokers with (n = 81) or without (n = 166) COPD; and (2) old ever smokers with (n = 159) or without (n = 29) COPD. A multivariable linear regression was used to assess the association of TL and mtDNA-CN with lung function. We observed that in the entire study population, TL and mtDNA-CN decreased with age, and the former but not the latter related to FEV1/FVC (%), FEV1 (% ref.), and DLCO (% ref.). The short telomeres were found both in the young and old patients with severe COPD (FEV1 <50% ref.). In addition, we found that TL and mtDNA-CN were significantly correlated, but their relationship was positive in younger while negative in the older patients with COPD, suggesting a mitochondrial dysfunction. We conclude that TL, but not mtDNA-CN, is associated with the lung function impairment. Both young and old patients with severe COPD have evidence of accelerated ageing (shorter TL) but differ in the direction of the correlation between TL and mtDNA-CN in relation to age. |
| format |
article |
| author |
Sandra Casas-Recasens Nuria Mendoza Alejandra López-Giraldo Alejandra López-Giraldo Alejandra López-Giraldo Tamara Garcia Borja G. Cosio Borja G. Cosio Borja G. Cosio Sergi Pascual-Guardia Sergi Pascual-Guardia Sergi Pascual-Guardia Ady Acosta-Castro Ady Acosta-Castro Alicia Borras-Santos Alicia Borras-Santos Joaquim Gea Joaquim Gea Joaquim Gea Gloria Garrabou Gloria Garrabou Gloria Garrabou Gloria Garrabou Alvar Agusti Alvar Agusti Alvar Agusti Alvar Agusti Rosa Faner Rosa Faner |
| author_facet |
Sandra Casas-Recasens Nuria Mendoza Alejandra López-Giraldo Alejandra López-Giraldo Alejandra López-Giraldo Tamara Garcia Borja G. Cosio Borja G. Cosio Borja G. Cosio Sergi Pascual-Guardia Sergi Pascual-Guardia Sergi Pascual-Guardia Ady Acosta-Castro Ady Acosta-Castro Alicia Borras-Santos Alicia Borras-Santos Joaquim Gea Joaquim Gea Joaquim Gea Gloria Garrabou Gloria Garrabou Gloria Garrabou Gloria Garrabou Alvar Agusti Alvar Agusti Alvar Agusti Alvar Agusti Rosa Faner Rosa Faner |
| author_sort |
Sandra Casas-Recasens |
| title |
Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| title_short |
Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| title_full |
Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| title_fullStr |
Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| title_full_unstemmed |
Telomere Length but Not Mitochondrial DNA Copy Number Is Altered in Both Young and Old COPD |
| title_sort |
telomere length but not mitochondrial dna copy number is altered in both young and old copd |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/21328096c5dc4b70aabf06761fa0ba5a |
| work_keys_str_mv |
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