Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.

In many in vitro experiments Brownian motion hampers quantitative data analysis. Therefore, additives are widely used to increase the solvent viscosity. For this purpose, methylcellulose (MC) has been proven highly effective as already small concentrations can significantly slow down diffusive proce...

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Autores principales:	Simone Köhler, Oliver Lieleg, Andreas R Bausch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2134b67ab9cf4532bbbbeaf32b50ee6a
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spelling	oai:doaj.org-article:2134b67ab9cf4532bbbbeaf32b50ee6a2021-11-25T06:11:33ZRheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.1932-620310.1371/journal.pone.0002736https://doaj.org/article/2134b67ab9cf4532bbbbeaf32b50ee6a2008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18629003/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In many in vitro experiments Brownian motion hampers quantitative data analysis. Therefore, additives are widely used to increase the solvent viscosity. For this purpose, methylcellulose (MC) has been proven highly effective as already small concentrations can significantly slow down diffusive processes. Beside this advantage, it has already been reported that high MC concentrations can alter the microstructure of polymer solutions such as filamentous actin. However, it remains to be shown to what extent the mechanical properties of a composite actin/MC gel depend on the MC concentration. In particular, significant alterations might occur even if the microstructure seems unaffected. Indeed, we find that the viscoelastic response of entangled F-actin solutions depends sensitively on the amount of MC added. At concentrations higher than 0.2% (w/v) MC, actin filaments are reorganized into bundles which drastically changes the viscoelastic response. At small MC concentrations the impact of MC is more subtle: the two constituents, actin and MC, contribute in an additive way to the mechanical response of the composite material. As a consequence, the effect of methylcellulose on actin solutions has to be considered very carefully when MC is used in biochemical experiments.Simone KöhlerOliver LielegAndreas R BauschPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2736 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Köhler Oliver Lieleg Andreas R Bausch Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
description	In many in vitro experiments Brownian motion hampers quantitative data analysis. Therefore, additives are widely used to increase the solvent viscosity. For this purpose, methylcellulose (MC) has been proven highly effective as already small concentrations can significantly slow down diffusive processes. Beside this advantage, it has already been reported that high MC concentrations can alter the microstructure of polymer solutions such as filamentous actin. However, it remains to be shown to what extent the mechanical properties of a composite actin/MC gel depend on the MC concentration. In particular, significant alterations might occur even if the microstructure seems unaffected. Indeed, we find that the viscoelastic response of entangled F-actin solutions depends sensitively on the amount of MC added. At concentrations higher than 0.2% (w/v) MC, actin filaments are reorganized into bundles which drastically changes the viscoelastic response. At small MC concentrations the impact of MC is more subtle: the two constituents, actin and MC, contribute in an additive way to the mechanical response of the composite material. As a consequence, the effect of methylcellulose on actin solutions has to be considered very carefully when MC is used in biochemical experiments.
format	article
author	Simone Köhler Oliver Lieleg Andreas R Bausch
author_facet	Simone Köhler Oliver Lieleg Andreas R Bausch
author_sort	Simone Köhler
title	Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
title_short	Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
title_full	Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
title_fullStr	Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
title_full_unstemmed	Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
title_sort	rheological characterization of the bundling transition in f-actin solutions induced by methylcellulose.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/2134b67ab9cf4532bbbbeaf32b50ee6a
work_keys_str_mv	AT simonekohler rheologicalcharacterizationofthebundlingtransitioninfactinsolutionsinducedbymethylcellulose AT oliverlieleg rheologicalcharacterizationofthebundlingtransitioninfactinsolutionsinducedbymethylcellulose AT andreasrbausch rheologicalcharacterizationofthebundlingtransitioninfactinsolutionsinducedbymethylcellulose
_version_	1718414030069039104

Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.

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