Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

Abstract Kidney organoids derived from the human pluripotent stem cells (hPSCs) recapitulating human kidney are the attractive tool for kidney regeneration, disease modeling, and drug screening. However, the kidney organoids cultured by static conditions have the limited vascular networks and immatu...

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Autores principales:	Han Na Lee, Yoon Young Choi, Jin Won Kim, Young Seo Lee, Ji Wook Choi, Taewook Kang, Yong Kyun Kim, Bong Guen Chung
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Kidney organoid-on-a-chip Shear stress Vascular structure Podocyte Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999
Acceso en línea:	https://doaj.org/article/2141839813ac4dbb82f1cef71577da65
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spelling	oai:doaj.org-article:2141839813ac4dbb82f1cef71577da652021-11-14T12:37:41ZEffect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip10.1186/s40580-021-00285-42196-5404https://doaj.org/article/2141839813ac4dbb82f1cef71577da652021-11-01T00:00:00Zhttps://doi.org/10.1186/s40580-021-00285-4https://doaj.org/toc/2196-5404Abstract Kidney organoids derived from the human pluripotent stem cells (hPSCs) recapitulating human kidney are the attractive tool for kidney regeneration, disease modeling, and drug screening. However, the kidney organoids cultured by static conditions have the limited vascular networks and immature nephron-like structures unlike human kidney. Here, we developed a kidney organoid-on-a-chip system providing fluidic flow mimicking shear stress with optimized extracellular matrix (ECM) conditions. We demonstrated that the kidney organoids cultured in our microfluidic system showed more matured podocytes and vascular structures as compared to the static culture condition. Additionally, the kidney organoids cultured in microfluidic systems showed higher sensitivity to nephrotoxic drugs as compared with those cultured in static conditions. We also demonstrated that the physiological flow played an important role in maintaining a number of physiological functions of kidney organoids. Therefore, our kidney organoid-on-a-chip system could provide an organoid culture platform for in vitro vascularization in formation of functional three-dimensional (3D) tissues.Han Na LeeYoon Young ChoiJin Won KimYoung Seo LeeJi Wook ChoiTaewook KangYong Kyun KimBong Guen ChungSpringerOpenarticleKidney organoid-on-a-chipShear stressVascular structurePodocyteTechnologyTChemical technologyTP1-1185BiotechnologyTP248.13-248.65ScienceQPhysicsQC1-999ENNano Convergence, Vol 8, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Kidney organoid-on-a-chip Shear stress Vascular structure Podocyte Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999
spellingShingle	Kidney organoid-on-a-chip Shear stress Vascular structure Podocyte Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Science Q Physics QC1-999 Han Na Lee Yoon Young Choi Jin Won Kim Young Seo Lee Ji Wook Choi Taewook Kang Yong Kyun Kim Bong Guen Chung Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
description	Abstract Kidney organoids derived from the human pluripotent stem cells (hPSCs) recapitulating human kidney are the attractive tool for kidney regeneration, disease modeling, and drug screening. However, the kidney organoids cultured by static conditions have the limited vascular networks and immature nephron-like structures unlike human kidney. Here, we developed a kidney organoid-on-a-chip system providing fluidic flow mimicking shear stress with optimized extracellular matrix (ECM) conditions. We demonstrated that the kidney organoids cultured in our microfluidic system showed more matured podocytes and vascular structures as compared to the static culture condition. Additionally, the kidney organoids cultured in microfluidic systems showed higher sensitivity to nephrotoxic drugs as compared with those cultured in static conditions. We also demonstrated that the physiological flow played an important role in maintaining a number of physiological functions of kidney organoids. Therefore, our kidney organoid-on-a-chip system could provide an organoid culture platform for in vitro vascularization in formation of functional three-dimensional (3D) tissues.
format	article
author	Han Na Lee Yoon Young Choi Jin Won Kim Young Seo Lee Ji Wook Choi Taewook Kang Yong Kyun Kim Bong Guen Chung
author_facet	Han Na Lee Yoon Young Choi Jin Won Kim Young Seo Lee Ji Wook Choi Taewook Kang Yong Kyun Kim Bong Guen Chung
author_sort	Han Na Lee
title	Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
title_short	Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
title_full	Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
title_fullStr	Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
title_full_unstemmed	Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
title_sort	effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/2141839813ac4dbb82f1cef71577da65
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Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

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