Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae

Acetochlor is one of the most widely used herbicides in the world, however, there are few data on the sub-lethal effects of acetochlor on early developmental stages of fish. To address this, we measured survival, deformity, swim bladder formation, embryo oxygen consumption rates, reactive oxygen spe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tao Huang, Shuo Wang, Christopher L. Souders, II, Emma Ivantsova, Andrew Wengrovitz, Jade Ganter, Yuan H. Zhao, Hongguang Cheng, Christopher J. Martyniuk
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Risk assessment Developmental toxicity Agrochemical Agriculture Aquatic species Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/21519d7fac8f4bd7b2cde0333c04b3a3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:21519d7fac8f4bd7b2cde0333c04b3a3
record_format	dspace
spelling	oai:doaj.org-article:21519d7fac8f4bd7b2cde0333c04b3a32021-11-18T04:43:15ZExposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae0147-651310.1016/j.ecoenv.2021.112978https://doaj.org/article/21519d7fac8f4bd7b2cde0333c04b3a32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010903https://doaj.org/toc/0147-6513Acetochlor is one of the most widely used herbicides in the world, however, there are few data on the sub-lethal effects of acetochlor on early developmental stages of fish. To address this, we measured survival, deformity, swim bladder formation, embryo oxygen consumption rates, reactive oxygen species (ROS) levels, transcripts (related to swim bladder formation, oxidative damage response, and apoptosis) and behavior responses following exposure to acetochlor (0.001 µM up to 125 µM). Exposure to acetochlor at concentrations 50 µM and above exerted 100% mortality after 3 dpf, and significantly reduced the size of the swim bladder (25 µM). In embryos, basal respiration, oligomycin-induced ATP production, and maximal respiration were decreased 30–60% following a 24 h exposure to 125 μM acetochlor. Acetochlor did not affect ROS levels up to 25 µM in larvae with acute exposure. Acetochlor at 25 µM increased mRNA levels of bax1, hsp70, and hsp90a by ~4-fold in larval zebrafish. In both the visual motor response and light-dark preference test, 25 µM acetochlor increased locomotor activity of larval fish. At lower exposure concentrations, 100 and 1000 nM acetochlor increased the mean time spent in the dark zone, suggesting promotion of anxiolytic behavior. This study presents a comprehensive evaluation of sublethal effects of acetochlor, spanning molecular responses to behavior, which can be used to refine risk assessment decisions for aquatic environments.Tao HuangShuo WangChristopher L. Souders, IIEmma IvantsovaAndrew WengrovitzJade GanterYuan H. ZhaoHongguang ChengChristopher J. MartyniukElsevierarticleRisk assessmentDevelopmental toxicityAgrochemicalAgricultureAquatic speciesEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112978- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Risk assessment Developmental toxicity Agrochemical Agriculture Aquatic species Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Risk assessment Developmental toxicity Agrochemical Agriculture Aquatic species Environmental pollution TD172-193.5 Environmental sciences GE1-350 Tao Huang Shuo Wang Christopher L. Souders, II Emma Ivantsova Andrew Wengrovitz Jade Ganter Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
description	Acetochlor is one of the most widely used herbicides in the world, however, there are few data on the sub-lethal effects of acetochlor on early developmental stages of fish. To address this, we measured survival, deformity, swim bladder formation, embryo oxygen consumption rates, reactive oxygen species (ROS) levels, transcripts (related to swim bladder formation, oxidative damage response, and apoptosis) and behavior responses following exposure to acetochlor (0.001 µM up to 125 µM). Exposure to acetochlor at concentrations 50 µM and above exerted 100% mortality after 3 dpf, and significantly reduced the size of the swim bladder (25 µM). In embryos, basal respiration, oligomycin-induced ATP production, and maximal respiration were decreased 30–60% following a 24 h exposure to 125 μM acetochlor. Acetochlor did not affect ROS levels up to 25 µM in larvae with acute exposure. Acetochlor at 25 µM increased mRNA levels of bax1, hsp70, and hsp90a by ~4-fold in larval zebrafish. In both the visual motor response and light-dark preference test, 25 µM acetochlor increased locomotor activity of larval fish. At lower exposure concentrations, 100 and 1000 nM acetochlor increased the mean time spent in the dark zone, suggesting promotion of anxiolytic behavior. This study presents a comprehensive evaluation of sublethal effects of acetochlor, spanning molecular responses to behavior, which can be used to refine risk assessment decisions for aquatic environments.
format	article
author	Tao Huang Shuo Wang Christopher L. Souders, II Emma Ivantsova Andrew Wengrovitz Jade Ganter Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk
author_facet	Tao Huang Shuo Wang Christopher L. Souders, II Emma Ivantsova Andrew Wengrovitz Jade Ganter Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk
author_sort	Tao Huang
title	Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
title_short	Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
title_full	Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
title_fullStr	Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
title_full_unstemmed	Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae
title_sort	exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (danio rerio) larvae
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/21519d7fac8f4bd7b2cde0333c04b3a3
work_keys_str_mv	AT taohuang exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT shuowang exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT christopherlsoudersii exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT emmaivantsova exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT andrewwengrovitz exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT jadeganter exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT yuanhzhao exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT hongguangcheng exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae AT christopherjmartyniuk exposuretoacetochlorimpairsswimbladderformationinducesheatshockproteinexpressionandpromoteslocomotoractivityinzebrafishdanioreriolarvae
_version_	1718425084981411840

Exposure to acetochlor impairs swim bladder formation, induces heat shock protein expression, and promotes locomotor activity in zebrafish (Danio rerio) larvae

Ejemplares similares