Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens

Narintorn Rattanata,1 Sompong Klaynongsruang,1 Chanvit Leelayuwat,2 Temduang Limpaiboon,2 Aroonlug Lulitanond,2 Patcharee Boonsiri,3 Sirinart Chio-Srichan,4 Siriwat Soontaranon,4 Supagorn Rugmai,4 Jureerut Daduang2 1Department of Biochemistry, Faculty of Science, 2Centre for Research and Development...

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Autores principales:	Rattanata N, Klaynongsruang S, Leelayuwat C, Limpaiboon T, Lulitanond A, Boonsiri P, Chio-Srichan S, Soontaranon S, Rugmai S, Daduang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	gold nanoparticles gallic acid antibacterial activity foodborne bacteria small-angle X-ray scattering (SAXS) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2158d1933ab14ea0b342a5a2796bf828
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spelling	oai:doaj.org-article:2158d1933ab14ea0b342a5a2796bf8282021-12-02T01:50:41ZGallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens1178-2013https://doaj.org/article/2158d1933ab14ea0b342a5a2796bf8282016-07-01T00:00:00Zhttps://www.dovepress.com/gallic-acid-conjugated-with-gold-nanoparticles-antibacterial-activity--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Narintorn Rattanata,1 Sompong Klaynongsruang,1 Chanvit Leelayuwat,2 Temduang Limpaiboon,2 Aroonlug Lulitanond,2 Patcharee Boonsiri,3 Sirinart Chio-Srichan,4 Siriwat Soontaranon,4 Supagorn Rugmai,4 Jureerut Daduang2 1Department of Biochemistry, Faculty of Science, 2Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, 3Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 4Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, Thailand Abstract: Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), are needed. Gold nanoparticles (AuNPs) are used as drug delivery agents. This study aimed to conjugate and characterize AuNP–GA and to evaluate the antibacterial activity. AuNP was conjugated with GA, and the core–shell structures were characterized by small-angle X-ray scattering and transmission electron microscopy. Antibacterial activity of AuNP–GA against P. shigelloides and S. flexneri B was evaluated by well diffusion method. AuNP–GA bactericidal mechanism was elucidated by Fourier transform infrared microspectroscopic analysis. The results of small-angle X-ray scattering showed that AuNP–GA conjugation was successful. Antibacterial activity of GA against both bacteria was improved by conjugation with AuNP because the minimum inhibitory concentration value of AuNP–GA was significantly decreased (P<0.0001) compared to that of GA. Fourier transform infrared analysis revealed that AuNP–GA resulted in alterations of lipids, proteins, and nucleic acids at the bacterial cell membrane. Our findings show that AuNP–GA has potential for further application in biomedical sciences.Keywords: gold nanoparticles, gallic acid, antibacterial activity, foodborne bacteria, small-angle X-ray scattering (SAXS) Rattanata NKlaynongsruang SLeelayuwat CLimpaiboon TLulitanond ABoonsiri PChio-Srichan SSoontaranon SRugmai SDaduang JDove Medical Pressarticlegold nanoparticlesgallic acidantibacterial activityfoodborne bacteriasmall-angle X-ray scattering (SAXS)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3347-3356 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticles gallic acid antibacterial activity foodborne bacteria small-angle X-ray scattering (SAXS) Medicine (General) R5-920
spellingShingle	gold nanoparticles gallic acid antibacterial activity foodborne bacteria small-angle X-ray scattering (SAXS) Medicine (General) R5-920 Rattanata N Klaynongsruang S Leelayuwat C Limpaiboon T Lulitanond A Boonsiri P Chio-Srichan S Soontaranon S Rugmai S Daduang J Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
description	Narintorn Rattanata,1 Sompong Klaynongsruang,1 Chanvit Leelayuwat,2 Temduang Limpaiboon,2 Aroonlug Lulitanond,2 Patcharee Boonsiri,3 Sirinart Chio-Srichan,4 Siriwat Soontaranon,4 Supagorn Rugmai,4 Jureerut Daduang2 1Department of Biochemistry, Faculty of Science, 2Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, 3Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 4Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, Thailand Abstract: Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), are needed. Gold nanoparticles (AuNPs) are used as drug delivery agents. This study aimed to conjugate and characterize AuNP–GA and to evaluate the antibacterial activity. AuNP was conjugated with GA, and the core–shell structures were characterized by small-angle X-ray scattering and transmission electron microscopy. Antibacterial activity of AuNP–GA against P. shigelloides and S. flexneri B was evaluated by well diffusion method. AuNP–GA bactericidal mechanism was elucidated by Fourier transform infrared microspectroscopic analysis. The results of small-angle X-ray scattering showed that AuNP–GA conjugation was successful. Antibacterial activity of GA against both bacteria was improved by conjugation with AuNP because the minimum inhibitory concentration value of AuNP–GA was significantly decreased (P<0.0001) compared to that of GA. Fourier transform infrared analysis revealed that AuNP–GA resulted in alterations of lipids, proteins, and nucleic acids at the bacterial cell membrane. Our findings show that AuNP–GA has potential for further application in biomedical sciences.Keywords: gold nanoparticles, gallic acid, antibacterial activity, foodborne bacteria, small-angle X-ray scattering (SAXS)
format	article
author	Rattanata N Klaynongsruang S Leelayuwat C Limpaiboon T Lulitanond A Boonsiri P Chio-Srichan S Soontaranon S Rugmai S Daduang J
author_facet	Rattanata N Klaynongsruang S Leelayuwat C Limpaiboon T Lulitanond A Boonsiri P Chio-Srichan S Soontaranon S Rugmai S Daduang J
author_sort	Rattanata N
title	Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
title_short	Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
title_full	Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
title_fullStr	Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
title_full_unstemmed	Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
title_sort	gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/2158d1933ab14ea0b342a5a2796bf828
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_version_	1718402820457103360

Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens

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