Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro

Abstract Background Currently, the prognosis of non‐small‐cell lung cancer (NSCLC) patients remains dismal due to recurrence and metastasis. The purpose of our study was to explore the role of circular RNA_0016760 (circ_0016760) in NSCLC progression and its associated mechanism. Methods Quantitative...

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Autores principales:	Shan Chen, Long Zhou, Ruizhi Ran, Jinqi Huang, Yong Zheng, Maohui Xing, Yanli Cai
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	AKT3 circ_0016760 miR‐646 non‐small‐cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/215b861112db4aa6a6e62cbf40da0a40
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spelling	oai:doaj.org-article:215b861112db4aa6a6e62cbf40da0a402021-12-02T02:34:55ZCirc_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro1759-77141759-770610.1111/1759-7714.14191https://doaj.org/article/215b861112db4aa6a6e62cbf40da0a402021-12-01T00:00:00Zhttps://doi.org/10.1111/1759-7714.14191https://doaj.org/toc/1759-7706https://doaj.org/toc/1759-7714Abstract Background Currently, the prognosis of non‐small‐cell lung cancer (NSCLC) patients remains dismal due to recurrence and metastasis. The purpose of our study was to explore the role of circular RNA_0016760 (circ_0016760) in NSCLC progression and its associated mechanism. Methods Quantitative real‐time polymerase chain reaction (qRT‐PCR) was implemented to measure the expression of circ_0016760, microRNA‐646 (miR‐646) and AK strain thymoma serine/threonine kinase 3 (AKT3). The protein level of AKT3 was examined by Western blot assay. Cell Counting Kit 8 assay, transwell assays, and flow cytometry were conducted to analyze cell proliferation, metastasis, and apoptosis. Dual‐luciferase reporter assay was used to confirm the interactions that were predicted by bioinformatics software (Circular RNA Interactome and TargetScan). A xenograft tumor model was built to investigate the role of circ_0016760 in vivo. Results Circ_0016760 and AKT3 were highly expressed in NSCLC tissue specimens and cell lines. Circ_0016760 interference suppressed cell proliferation, migration, and invasion and promoted the apoptosis of NSCLC cells. Circ_0016760 interacted with miR‐646 and negatively regulated its expression. MiR‐646 silencing partly counteracted circ_0016760 knockdown‐mediated influences in NSCLC cells. MiR‐646 bound to the AKT3 3′ untranslated region in NSCLC cells, and miR‐646 overexpression‐induced effects in NSCLC cells were partly overturned by the addition of AKT3 overexpression plasmid. Circ_0016760 silencing reduced the expression of AKT3 through enhancing miR‐646 expression. Circ_0016760 knockdown suppressed NSCLC tumor growth in vivo. Conclusion Circ_0016760 played an oncogenic role to promote the proliferation, migration, and invasion and restrained the apoptosis of NSCLC cells via miR‐646/AKT3 signaling.Shan ChenLong ZhouRuizhi RanJinqi HuangYong ZhengMaohui XingYanli CaiWileyarticleAKT3circ_0016760miR‐646non‐small‐cell lung cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENThoracic Cancer, Vol 12, Iss 23, Pp 3223-3235 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	AKT3 circ_0016760 miR‐646 non‐small‐cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	AKT3 circ_0016760 miR‐646 non‐small‐cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Shan Chen Long Zhou Ruizhi Ran Jinqi Huang Yong Zheng Maohui Xing Yanli Cai Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
description	Abstract Background Currently, the prognosis of non‐small‐cell lung cancer (NSCLC) patients remains dismal due to recurrence and metastasis. The purpose of our study was to explore the role of circular RNA_0016760 (circ_0016760) in NSCLC progression and its associated mechanism. Methods Quantitative real‐time polymerase chain reaction (qRT‐PCR) was implemented to measure the expression of circ_0016760, microRNA‐646 (miR‐646) and AK strain thymoma serine/threonine kinase 3 (AKT3). The protein level of AKT3 was examined by Western blot assay. Cell Counting Kit 8 assay, transwell assays, and flow cytometry were conducted to analyze cell proliferation, metastasis, and apoptosis. Dual‐luciferase reporter assay was used to confirm the interactions that were predicted by bioinformatics software (Circular RNA Interactome and TargetScan). A xenograft tumor model was built to investigate the role of circ_0016760 in vivo. Results Circ_0016760 and AKT3 were highly expressed in NSCLC tissue specimens and cell lines. Circ_0016760 interference suppressed cell proliferation, migration, and invasion and promoted the apoptosis of NSCLC cells. Circ_0016760 interacted with miR‐646 and negatively regulated its expression. MiR‐646 silencing partly counteracted circ_0016760 knockdown‐mediated influences in NSCLC cells. MiR‐646 bound to the AKT3 3′ untranslated region in NSCLC cells, and miR‐646 overexpression‐induced effects in NSCLC cells were partly overturned by the addition of AKT3 overexpression plasmid. Circ_0016760 silencing reduced the expression of AKT3 through enhancing miR‐646 expression. Circ_0016760 knockdown suppressed NSCLC tumor growth in vivo. Conclusion Circ_0016760 played an oncogenic role to promote the proliferation, migration, and invasion and restrained the apoptosis of NSCLC cells via miR‐646/AKT3 signaling.
format	article
author	Shan Chen Long Zhou Ruizhi Ran Jinqi Huang Yong Zheng Maohui Xing Yanli Cai
author_facet	Shan Chen Long Zhou Ruizhi Ran Jinqi Huang Yong Zheng Maohui Xing Yanli Cai
author_sort	Shan Chen
title	Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
title_short	Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
title_full	Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
title_fullStr	Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
title_full_unstemmed	Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro
title_sort	circ_0016760 accelerates non‐small‐cell lung cancer progression through mir‐646/akt3 signaling in vivo and in vitro
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/215b861112db4aa6a6e62cbf40da0a40
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Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro

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