The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.

One of the hallmarks of opportunistic pathogens is their ability to adjust and respond to a wide range of environmental and host-associated conditions. The human pathogen Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic infections in indivi...

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Autores principales:	Omri Wurtzel, Deborah R Yoder-Himes, Kook Han, Ajai A Dandekar, Sarit Edelheit, E Peter Greenberg, Rotem Sorek, Stephen Lory
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/215be2ac9dda4370ae88d1aa7a674d8a
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spelling	oai:doaj.org-article:215be2ac9dda4370ae88d1aa7a674d8a2021-11-18T06:03:55ZThe single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.1553-73661553-737410.1371/journal.ppat.1002945https://doaj.org/article/215be2ac9dda4370ae88d1aa7a674d8a2012-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028334/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374One of the hallmarks of opportunistic pathogens is their ability to adjust and respond to a wide range of environmental and host-associated conditions. The human pathogen Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic infections in individuals with impaired host defenses or cystic fibrosis. Here we report an in-depth transcriptional profiling of this organism when grown at host-related temperatures. Using RNA-seq of samples from P. aeruginosa grown at 28°C and 37°C we detected genes preferentially expressed at the body temperature of mammalian hosts, suggesting that they play a role during infection. These temperature-induced genes included the type III secretion system (T3SS) genes and effectors, as well as the genes responsible for phenazines biosynthesis. Using genome-wide transcription start site (TSS) mapping by RNA-seq we were able to accurately define the promoters and cis-acting RNA elements of many genes, and uncovered new genes and previously unrecognized non-coding RNAs directly controlled by the LasR quorum sensing regulator. Overall we identified 165 small RNAs and over 380 cis-antisense RNAs, some of which predicted to perform regulatory functions, and found that non-coding RNAs are preferentially localized in pathogenicity islands and horizontally transferred regions. Our work identifies regulatory features of P. aeruginosa genes whose products play a role in environmental adaption during infection and provides a reference transcriptional landscape for this pathogen.Omri WurtzelDeborah R Yoder-HimesKook HanAjai A DandekarSarit EdelheitE Peter GreenbergRotem SorekStephen LoryPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 9, p e1002945 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Omri Wurtzel Deborah R Yoder-Himes Kook Han Ajai A Dandekar Sarit Edelheit E Peter Greenberg Rotem Sorek Stephen Lory The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
description	One of the hallmarks of opportunistic pathogens is their ability to adjust and respond to a wide range of environmental and host-associated conditions. The human pathogen Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic infections in individuals with impaired host defenses or cystic fibrosis. Here we report an in-depth transcriptional profiling of this organism when grown at host-related temperatures. Using RNA-seq of samples from P. aeruginosa grown at 28°C and 37°C we detected genes preferentially expressed at the body temperature of mammalian hosts, suggesting that they play a role during infection. These temperature-induced genes included the type III secretion system (T3SS) genes and effectors, as well as the genes responsible for phenazines biosynthesis. Using genome-wide transcription start site (TSS) mapping by RNA-seq we were able to accurately define the promoters and cis-acting RNA elements of many genes, and uncovered new genes and previously unrecognized non-coding RNAs directly controlled by the LasR quorum sensing regulator. Overall we identified 165 small RNAs and over 380 cis-antisense RNAs, some of which predicted to perform regulatory functions, and found that non-coding RNAs are preferentially localized in pathogenicity islands and horizontally transferred regions. Our work identifies regulatory features of P. aeruginosa genes whose products play a role in environmental adaption during infection and provides a reference transcriptional landscape for this pathogen.
format	article
author	Omri Wurtzel Deborah R Yoder-Himes Kook Han Ajai A Dandekar Sarit Edelheit E Peter Greenberg Rotem Sorek Stephen Lory
author_facet	Omri Wurtzel Deborah R Yoder-Himes Kook Han Ajai A Dandekar Sarit Edelheit E Peter Greenberg Rotem Sorek Stephen Lory
author_sort	Omri Wurtzel
title	The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
title_short	The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
title_full	The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
title_fullStr	The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
title_full_unstemmed	The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.
title_sort	single-nucleotide resolution transcriptome of pseudomonas aeruginosa grown in body temperature.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/215be2ac9dda4370ae88d1aa7a674d8a
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The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature.

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