Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats

The present study is to clarify the effect of insulin-producing cells (IPCs) derived from adipose tissue mesenchymal stem cells (AT-MSCs) on diabetic-induced impairments as the abnormalities of testicular tissues, oxidative stress of testes, and defects of spermatogenesis. Diabetes was stimulated by...

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Autores principales:	Mai A. Abd El Kader, Mahmoud M. Gabr, Sherry M. Khater, Reham A. Ghanem, Amoura M. Abou El Naga
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Diabetes STZ Stem cells Subcutaneous transplantation TheraCyte capsule Testicular dysfunction Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/215f81893e33418d948ae751610d9ee2
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spelling	oai:doaj.org-article:215f81893e33418d948ae751610d9ee22021-12-02T05:02:36ZImpact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats2405-844010.1016/j.heliyon.2021.e08316https://doaj.org/article/215f81893e33418d948ae751610d9ee22021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024191https://doaj.org/toc/2405-8440The present study is to clarify the effect of insulin-producing cells (IPCs) derived from adipose tissue mesenchymal stem cells (AT-MSCs) on diabetic-induced impairments as the abnormalities of testicular tissues, oxidative stress of testes, and defects of spermatogenesis. Diabetes was stimulated by streptozotocin (STZ) injection in male adult Sprague Dawley (SD) rats. Diabetes was confirmed by taking two highly consecutive fasting blood sugar readings; more than 300 mg/dl; within one week. Five million of IPCs derived from AT-MSCs; encased in TheraCyte capsule; were then directly transplanted (one implant for each rat) subcutaneously in diabetic rats. Implants were maintained for 3 months and the fasting blood sugar of the transplanted rats was observed every month. At the end of the experiment; serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were also estimated. The sperm parameters (count, motility, and abnormality) were recorded. In testicular tissue; GPX4, Bcl2, and Bax levels were evaluated, while oxidative stress and antioxidant enzymes activities were measured in the testes homogenate. Also, histopathological alterations were examined in the testes cross-section. In the results, it was found that IPCs treatment enhanced the serum testosterone, FSH, and LH levels. Diabetic-induced impairments in the sperm parameters were noticeably improved post-IPCs transplantation in the diabetic rats. Moreover, the treatment improved the diabetic-associated testicular oxidative stress. Also, it was recognized that the Bax expression decreased, while, GPX4 and Bcl2 expression increased in the treated rats. Meanwhile, the abnormalities showed in the histopathological studies of the hyperglycemic rat's testes were attenuated post-treatment. So, IPCs transplantation improved diabetes and consequently protected against hyperglycemia-induced testicular damages.Mai A. Abd El KaderMahmoud M. GabrSherry M. KhaterReham A. GhanemAmoura M. Abou El NagaElsevierarticleDiabetesSTZStem cellsSubcutaneous transplantationTheraCyte capsuleTesticular dysfunctionScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08316- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diabetes STZ Stem cells Subcutaneous transplantation TheraCyte capsule Testicular dysfunction Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Diabetes STZ Stem cells Subcutaneous transplantation TheraCyte capsule Testicular dysfunction Science (General) Q1-390 Social sciences (General) H1-99 Mai A. Abd El Kader Mahmoud M. Gabr Sherry M. Khater Reham A. Ghanem Amoura M. Abou El Naga Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
description	The present study is to clarify the effect of insulin-producing cells (IPCs) derived from adipose tissue mesenchymal stem cells (AT-MSCs) on diabetic-induced impairments as the abnormalities of testicular tissues, oxidative stress of testes, and defects of spermatogenesis. Diabetes was stimulated by streptozotocin (STZ) injection in male adult Sprague Dawley (SD) rats. Diabetes was confirmed by taking two highly consecutive fasting blood sugar readings; more than 300 mg/dl; within one week. Five million of IPCs derived from AT-MSCs; encased in TheraCyte capsule; were then directly transplanted (one implant for each rat) subcutaneously in diabetic rats. Implants were maintained for 3 months and the fasting blood sugar of the transplanted rats was observed every month. At the end of the experiment; serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were also estimated. The sperm parameters (count, motility, and abnormality) were recorded. In testicular tissue; GPX4, Bcl2, and Bax levels were evaluated, while oxidative stress and antioxidant enzymes activities were measured in the testes homogenate. Also, histopathological alterations were examined in the testes cross-section. In the results, it was found that IPCs treatment enhanced the serum testosterone, FSH, and LH levels. Diabetic-induced impairments in the sperm parameters were noticeably improved post-IPCs transplantation in the diabetic rats. Moreover, the treatment improved the diabetic-associated testicular oxidative stress. Also, it was recognized that the Bax expression decreased, while, GPX4 and Bcl2 expression increased in the treated rats. Meanwhile, the abnormalities showed in the histopathological studies of the hyperglycemic rat's testes were attenuated post-treatment. So, IPCs transplantation improved diabetes and consequently protected against hyperglycemia-induced testicular damages.
format	article
author	Mai A. Abd El Kader Mahmoud M. Gabr Sherry M. Khater Reham A. Ghanem Amoura M. Abou El Naga
author_facet	Mai A. Abd El Kader Mahmoud M. Gabr Sherry M. Khater Reham A. Ghanem Amoura M. Abou El Naga
author_sort	Mai A. Abd El Kader
title	Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
title_short	Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
title_full	Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
title_fullStr	Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
title_full_unstemmed	Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
title_sort	impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/215f81893e33418d948ae751610d9ee2
work_keys_str_mv	AT maiaabdelkader impactofinsulinproducingcellsderivedfromadiposetissuemesenchymalstemcellsontesticulardysfunctionofdiabeticrats AT mahmoudmgabr impactofinsulinproducingcellsderivedfromadiposetissuemesenchymalstemcellsontesticulardysfunctionofdiabeticrats AT sherrymkhater impactofinsulinproducingcellsderivedfromadiposetissuemesenchymalstemcellsontesticulardysfunctionofdiabeticrats AT rehamaghanem impactofinsulinproducingcellsderivedfromadiposetissuemesenchymalstemcellsontesticulardysfunctionofdiabeticrats AT amouramabouelnaga impactofinsulinproducingcellsderivedfromadiposetissuemesenchymalstemcellsontesticulardysfunctionofdiabeticrats
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Impact of insulin producing cells derived from adipose tissue mesenchymal stem cells on testicular dysfunction of diabetic rats

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