Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.

<h4>Background</h4>Schistosoma infection is thought to lead to down-regulation of the host's immune response. This has been shown for adaptive immune responses, but the effect on innate immunity, that initiates and shapes the adaptive response, has not been extensively studied. In a...

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Autores principales:	Lynn Meurs, Lucja Labuda, Abena Serwaa Amoah, Moustapha Mbow, Ulysse Ateba Ngoa, Daniel Adjei Boakye, Souleymane Mboup, Tandakha Ndiaye Dièye, Adrian P Mountford, Joseph D Turner, Peter Gottfried Kremsner, Katja Polman, Maria Yazdanbakhsh, Ayola Akim Adegnika
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/216218e08e8f4e8fb30075f04f340b4d
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spelling	oai:doaj.org-article:216218e08e8f4e8fb30075f04f340b4d2021-11-04T06:09:01ZEnhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.1932-620310.1371/journal.pone.0024393https://doaj.org/article/216218e08e8f4e8fb30075f04f340b4d2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21931706/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Schistosoma infection is thought to lead to down-regulation of the host's immune response. This has been shown for adaptive immune responses, but the effect on innate immunity, that initiates and shapes the adaptive response, has not been extensively studied. In a first study to characterize these responses, we investigated the effect of Schistosoma haematobium infection on cytokine responses of Gabonese schoolchildren to a number of Toll-like receptor (TLR) ligands.<h4>Methodology</h4>Peripheral blood mononuclear cells (PBMCs) were collected from S. haematobium-infected and uninfected schoolchildren from the rural area of Zilé in Gabon. PBMCs were incubated for 24 h and 72 h with various TLR ligands, as well as schistosomal egg antigen (SEA) and adult worm antigen (AWA). Pro-inflammatory TNF-α and anti-inflammatory/regulatory IL-10 cytokine concentrations were determined in culture supernatants.<h4>Principal findings</h4>Infected children produced higher adaptive IL-10 responses than uninfected children against schistosomal antigens (72 h incubation). On the other hand, infected children had higher TNF-α responses than uninfected children and significantly higher TNF-α to IL-10 ratios in response to FSL-1 and Pam3, ligands of TLR2/6 and TLR2/1 respectively. A similar trend was observed for the TLR4 ligand LPS while Poly(I:C) (Mda5/TLR3 ligand) did not induce substantial cytokine responses (24 h incubation).<h4>Conclusions</h4>This pilot study shows that Schistosoma-infected children develop a more pro-inflammatory TLR2-mediated response in the face of a more anti-inflammatory adaptive immune response. This suggests that S. haematobium infection does not suppress the host's innate immune system in the context of single TLR ligation.Lynn MeursLucja LabudaAbena Serwaa AmoahMoustapha MbowUlysse Ateba NgoaDaniel Adjei BoakyeSouleymane MboupTandakha Ndiaye DièyeAdrian P MountfordJoseph D TurnerPeter Gottfried KremsnerKatja PolmanMaria YazdanbakhshAyola Akim AdegnikaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24393 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lynn Meurs Lucja Labuda Abena Serwaa Amoah Moustapha Mbow Ulysse Ateba Ngoa Daniel Adjei Boakye Souleymane Mboup Tandakha Ndiaye Dièye Adrian P Mountford Joseph D Turner Peter Gottfried Kremsner Katja Polman Maria Yazdanbakhsh Ayola Akim Adegnika Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
description	<h4>Background</h4>Schistosoma infection is thought to lead to down-regulation of the host's immune response. This has been shown for adaptive immune responses, but the effect on innate immunity, that initiates and shapes the adaptive response, has not been extensively studied. In a first study to characterize these responses, we investigated the effect of Schistosoma haematobium infection on cytokine responses of Gabonese schoolchildren to a number of Toll-like receptor (TLR) ligands.<h4>Methodology</h4>Peripheral blood mononuclear cells (PBMCs) were collected from S. haematobium-infected and uninfected schoolchildren from the rural area of Zilé in Gabon. PBMCs were incubated for 24 h and 72 h with various TLR ligands, as well as schistosomal egg antigen (SEA) and adult worm antigen (AWA). Pro-inflammatory TNF-α and anti-inflammatory/regulatory IL-10 cytokine concentrations were determined in culture supernatants.<h4>Principal findings</h4>Infected children produced higher adaptive IL-10 responses than uninfected children against schistosomal antigens (72 h incubation). On the other hand, infected children had higher TNF-α responses than uninfected children and significantly higher TNF-α to IL-10 ratios in response to FSL-1 and Pam3, ligands of TLR2/6 and TLR2/1 respectively. A similar trend was observed for the TLR4 ligand LPS while Poly(I:C) (Mda5/TLR3 ligand) did not induce substantial cytokine responses (24 h incubation).<h4>Conclusions</h4>This pilot study shows that Schistosoma-infected children develop a more pro-inflammatory TLR2-mediated response in the face of a more anti-inflammatory adaptive immune response. This suggests that S. haematobium infection does not suppress the host's innate immune system in the context of single TLR ligation.
format	article
author	Lynn Meurs Lucja Labuda Abena Serwaa Amoah Moustapha Mbow Ulysse Ateba Ngoa Daniel Adjei Boakye Souleymane Mboup Tandakha Ndiaye Dièye Adrian P Mountford Joseph D Turner Peter Gottfried Kremsner Katja Polman Maria Yazdanbakhsh Ayola Akim Adegnika
author_facet	Lynn Meurs Lucja Labuda Abena Serwaa Amoah Moustapha Mbow Ulysse Ateba Ngoa Daniel Adjei Boakye Souleymane Mboup Tandakha Ndiaye Dièye Adrian P Mountford Joseph D Turner Peter Gottfried Kremsner Katja Polman Maria Yazdanbakhsh Ayola Akim Adegnika
author_sort	Lynn Meurs
title	Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
title_short	Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
title_full	Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
title_fullStr	Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
title_full_unstemmed	Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.
title_sort	enhanced pro-inflammatory cytokine responses following toll-like-receptor ligation in schistosoma haematobium-infected schoolchildren from rural gabon.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/216218e08e8f4e8fb30075f04f340b4d
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Enhanced pro-inflammatory cytokine responses following Toll-like-receptor ligation in Schistosoma haematobium-infected schoolchildren from rural Gabon.

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