Role of exosomal miRNAs in brain metastasis affected by radiotherapy

In oncogenesis and development of malignant tumor, microRNAs (miRNAs) regulate the complex gene expression associated with the tumor pathogenesis. Currently, only few studies have been conducted to identify miRNAs and the potential pathways involved in the pathogenesis of brain metastasis in patient...

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Autores principales:	Li Zihuang, Yang Hongli, Ye Ling, Quan Rencui, Chen Meili
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	radiotherapy brain metastasis exosome micrornas malignant tumors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/21621a9975704d01a17bf56315928de3
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spelling	oai:doaj.org-article:21621a9975704d01a17bf56315928de32021-12-05T14:11:05ZRole of exosomal miRNAs in brain metastasis affected by radiotherapy2081-693610.1515/tnsci-2020-0163https://doaj.org/article/21621a9975704d01a17bf56315928de32021-03-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0163https://doaj.org/toc/2081-6936In oncogenesis and development of malignant tumor, microRNAs (miRNAs) regulate the complex gene expression associated with the tumor pathogenesis. Currently, only few studies have been conducted to identify miRNAs and the potential pathways involved in the pathogenesis of brain metastasis in patients who underwent radiotherapy, especially miRNAs in the plasma exosomes. Therefore, this study is aimed to use small RNA analysis to identify miRNAs and their potential target genes in plasma exosomes during the initiation and development of brain metastasis in patients who underwent radiotherapy. Using high-throughput sequencing technologies, we identified 35 differentially expressed miRNAs in patients with brain metastasis who had undergone radiotherapy. In annotation of miRNA targets, gene ontology enrichment analysis revealed that the targets of the differentially expressed miRNAs were significantly enriched in the regulation of cellular processes. Kyoto Encyclopedia of Genes and Genomes revealed that most of the miRNA targets were cancer-related, including genes involved in the mitogen-activated protein kinase signaling pathway, cancer-related pathways, phosphatidylinositol 3-kinase-protein kinase B signaling pathway, microtubule-associated protein kinase signaling pathway, Ras signaling pathway, regulation of the actin cytoskeleton, and axon guidance. In conclusion, this study provides a new perspective to understand the possible function of these miRNAs in the pathogenesis of brain metastasis. This was the first time that a pilot study identified plasma exosomal miRNAs in five patients with brain metastasis before and after radiotherapy. This study is the beginning; more specimen and further research are needed to explore the functional role of specific miRNAs and their potential as therapeutic targets for brain metastasis.Li ZihuangYang HongliYe LingQuan RencuiChen MeiliDe Gruyterarticleradiotherapybrain metastasisexosomemicrornasmalignant tumorsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 127-137 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	radiotherapy brain metastasis exosome micrornas malignant tumors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	radiotherapy brain metastasis exosome micrornas malignant tumors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Li Zihuang Yang Hongli Ye Ling Quan Rencui Chen Meili Role of exosomal miRNAs in brain metastasis affected by radiotherapy
description	In oncogenesis and development of malignant tumor, microRNAs (miRNAs) regulate the complex gene expression associated with the tumor pathogenesis. Currently, only few studies have been conducted to identify miRNAs and the potential pathways involved in the pathogenesis of brain metastasis in patients who underwent radiotherapy, especially miRNAs in the plasma exosomes. Therefore, this study is aimed to use small RNA analysis to identify miRNAs and their potential target genes in plasma exosomes during the initiation and development of brain metastasis in patients who underwent radiotherapy. Using high-throughput sequencing technologies, we identified 35 differentially expressed miRNAs in patients with brain metastasis who had undergone radiotherapy. In annotation of miRNA targets, gene ontology enrichment analysis revealed that the targets of the differentially expressed miRNAs were significantly enriched in the regulation of cellular processes. Kyoto Encyclopedia of Genes and Genomes revealed that most of the miRNA targets were cancer-related, including genes involved in the mitogen-activated protein kinase signaling pathway, cancer-related pathways, phosphatidylinositol 3-kinase-protein kinase B signaling pathway, microtubule-associated protein kinase signaling pathway, Ras signaling pathway, regulation of the actin cytoskeleton, and axon guidance. In conclusion, this study provides a new perspective to understand the possible function of these miRNAs in the pathogenesis of brain metastasis. This was the first time that a pilot study identified plasma exosomal miRNAs in five patients with brain metastasis before and after radiotherapy. This study is the beginning; more specimen and further research are needed to explore the functional role of specific miRNAs and their potential as therapeutic targets for brain metastasis.
format	article
author	Li Zihuang Yang Hongli Ye Ling Quan Rencui Chen Meili
author_facet	Li Zihuang Yang Hongli Ye Ling Quan Rencui Chen Meili
author_sort	Li Zihuang
title	Role of exosomal miRNAs in brain metastasis affected by radiotherapy
title_short	Role of exosomal miRNAs in brain metastasis affected by radiotherapy
title_full	Role of exosomal miRNAs in brain metastasis affected by radiotherapy
title_fullStr	Role of exosomal miRNAs in brain metastasis affected by radiotherapy
title_full_unstemmed	Role of exosomal miRNAs in brain metastasis affected by radiotherapy
title_sort	role of exosomal mirnas in brain metastasis affected by radiotherapy
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/21621a9975704d01a17bf56315928de3
work_keys_str_mv	AT lizihuang roleofexosomalmirnasinbrainmetastasisaffectedbyradiotherapy AT yanghongli roleofexosomalmirnasinbrainmetastasisaffectedbyradiotherapy AT yeling roleofexosomalmirnasinbrainmetastasisaffectedbyradiotherapy AT quanrencui roleofexosomalmirnasinbrainmetastasisaffectedbyradiotherapy AT chenmeili roleofexosomalmirnasinbrainmetastasisaffectedbyradiotherapy
_version_	1718371407432253440

Role of exosomal miRNAs in brain metastasis affected by radiotherapy

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