Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types

Abstract Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substan...

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Autores principales:	R. A. Barclay, I. Akhrymuk, A. Patnaik, V. Callahan, C. Lehman, P. Andersen, R. Barbero, S. Barksdale, R. Dunlap, D. Goldfarb, T. Jones-Roe, R. Kelly, B. Kim, S. Miao, A. Munns, D. Munns, S. Patel, E. Porter, R. Ramsey, S. Sahoo, O. Swahn, J. Warsh, K. Kehn-Hall, B. Lepene
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2166ba090ca845df90802b4e2de1dc81
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spelling	oai:doaj.org-article:2166ba090ca845df90802b4e2de1dc812021-12-02T14:01:28ZHydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types10.1038/s41598-020-78771-82045-2322https://doaj.org/article/2166ba090ca845df90802b4e2de1dc812020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78771-8https://doaj.org/toc/2045-2322Abstract Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100–1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL.R. A. BarclayI. AkhrymukA. PatnaikV. CallahanC. LehmanP. AndersenR. BarberoS. BarksdaleR. DunlapD. GoldfarbT. Jones-RoeR. KellyB. KimS. MiaoA. MunnsD. MunnsS. PatelE. PorterR. RamseyS. SahooO. SwahnJ. WarshK. Kehn-HallB. LepeneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. A. Barclay I. Akhrymuk A. Patnaik V. Callahan C. Lehman P. Andersen R. Barbero S. Barksdale R. Dunlap D. Goldfarb T. Jones-Roe R. Kelly B. Kim S. Miao A. Munns D. Munns S. Patel E. Porter R. Ramsey S. Sahoo O. Swahn J. Warsh K. Kehn-Hall B. Lepene Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
description	Abstract Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100–1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL.
format	article
author	R. A. Barclay I. Akhrymuk A. Patnaik V. Callahan C. Lehman P. Andersen R. Barbero S. Barksdale R. Dunlap D. Goldfarb T. Jones-Roe R. Kelly B. Kim S. Miao A. Munns D. Munns S. Patel E. Porter R. Ramsey S. Sahoo O. Swahn J. Warsh K. Kehn-Hall B. Lepene
author_facet	R. A. Barclay I. Akhrymuk A. Patnaik V. Callahan C. Lehman P. Andersen R. Barbero S. Barksdale R. Dunlap D. Goldfarb T. Jones-Roe R. Kelly B. Kim S. Miao A. Munns D. Munns S. Patel E. Porter R. Ramsey S. Sahoo O. Swahn J. Warsh K. Kehn-Hall B. Lepene
author_sort	R. A. Barclay
title	Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
title_short	Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
title_full	Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
title_fullStr	Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
title_full_unstemmed	Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
title_sort	hydrogel particles improve detection of sars-cov-2 rna from multiple sample types
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2166ba090ca845df90802b4e2de1dc81
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Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types

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