Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS

Quinones and humus are ubiquitous in the biosphere and play an important role in the anaerobic biodegradation and biotransformation of organic acids, poisonous compounds as well as inorganic compounds. The impact of humic model compound, anthraquinone-2, 6-disulfonate (AQDS) on anaerobic phenol and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mingmei Chi, Xiaoli Su, Xiaojiao Sun, Yan Xu, Xiaoxia Wang, Yanling Qiu
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	anaerobic syntrophism aqds cryptanaerobacter phenolicus phenols degradation syntrophorhabdus aromaticivorans Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/216d443c5fc74706ab7c972140a4911b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:216d443c5fc74706ab7c972140a4911b
record_format	dspace
spelling	oai:doaj.org-article:216d443c5fc74706ab7c972140a4911b2021-11-06T11:16:39ZMicrobial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS0273-12231996-973210.2166/wst.2021.264https://doaj.org/article/216d443c5fc74706ab7c972140a4911b2021-08-01T00:00:00Zhttp://wst.iwaponline.com/content/84/3/683https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Quinones and humus are ubiquitous in the biosphere and play an important role in the anaerobic biodegradation and biotransformation of organic acids, poisonous compounds as well as inorganic compounds. The impact of humic model compound, anthraquinone-2, 6-disulfonate (AQDS) on anaerobic phenol and p-cresol degradation were studied. Four methanogenic AQDS-free phenol and p-cresol enrichments and two phenol-AQDS enrichments were obtained using two sludges with potential biodegradability of phenol and cresol isomers as inoculum. 16S rRNA gene-cloning analysis combined with fluorescence in situ hybridization revealed that syntrophic aromatic compound degrading bacterium Syntrophorhabdus aromaticivorans was dominant in four AQDS-free enrichments, whereas phenol degrading Cryptanaerobacter phenolicus was dominant in two phenol-AQDS enrichments. Neither co-culture of S. aromaticivorans with Methanospirillum hungatei nor two phenol-AQDS enrichments could metabolize phenol using AQDS as the terminal electron acceptor. Further degradation experiments suggested that C. phenolicus related microbes in two phenol-AQDS enrichments were responsible for the conversion of phenol to benzoate, and benzoate was further degraded by benzoate degraders of Syntrophus aciditrophicus or Sporotomaculum syntrophicum to acetate. HIGHLIGHTS Anaerobic degradation of phenol and p-cresol were faster than o- and m-cresols.; 2 mM AQDS mitigated phenol and completely inhibited p-cresol degradation.; The dominant bacteria in AQDS-free and phenol-AQDS enrichments were different.; Neither S. aromaticivorans nor C. phenolicus affinities could use AQDS as TEA.; The characteristic of key phenol degraders in phenol-AQDS enrichments was described.;Mingmei ChiXiaoli SuXiaojiao SunYan XuXiaoxia WangYanling QiuIWA Publishingarticleanaerobic syntrophismaqdscryptanaerobacter phenolicusphenols degradationsyntrophorhabdus aromaticivoransEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 3, Pp 683-696 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anaerobic syntrophism aqds cryptanaerobacter phenolicus phenols degradation syntrophorhabdus aromaticivorans Environmental technology. Sanitary engineering TD1-1066
spellingShingle	anaerobic syntrophism aqds cryptanaerobacter phenolicus phenols degradation syntrophorhabdus aromaticivorans Environmental technology. Sanitary engineering TD1-1066 Mingmei Chi Xiaoli Su Xiaojiao Sun Yan Xu Xiaoxia Wang Yanling Qiu Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
description	Quinones and humus are ubiquitous in the biosphere and play an important role in the anaerobic biodegradation and biotransformation of organic acids, poisonous compounds as well as inorganic compounds. The impact of humic model compound, anthraquinone-2, 6-disulfonate (AQDS) on anaerobic phenol and p-cresol degradation were studied. Four methanogenic AQDS-free phenol and p-cresol enrichments and two phenol-AQDS enrichments were obtained using two sludges with potential biodegradability of phenol and cresol isomers as inoculum. 16S rRNA gene-cloning analysis combined with fluorescence in situ hybridization revealed that syntrophic aromatic compound degrading bacterium Syntrophorhabdus aromaticivorans was dominant in four AQDS-free enrichments, whereas phenol degrading Cryptanaerobacter phenolicus was dominant in two phenol-AQDS enrichments. Neither co-culture of S. aromaticivorans with Methanospirillum hungatei nor two phenol-AQDS enrichments could metabolize phenol using AQDS as the terminal electron acceptor. Further degradation experiments suggested that C. phenolicus related microbes in two phenol-AQDS enrichments were responsible for the conversion of phenol to benzoate, and benzoate was further degraded by benzoate degraders of Syntrophus aciditrophicus or Sporotomaculum syntrophicum to acetate. HIGHLIGHTS Anaerobic degradation of phenol and p-cresol were faster than o- and m-cresols.; 2 mM AQDS mitigated phenol and completely inhibited p-cresol degradation.; The dominant bacteria in AQDS-free and phenol-AQDS enrichments were different.; Neither S. aromaticivorans nor C. phenolicus affinities could use AQDS as TEA.; The characteristic of key phenol degraders in phenol-AQDS enrichments was described.;
format	article
author	Mingmei Chi Xiaoli Su Xiaojiao Sun Yan Xu Xiaoxia Wang Yanling Qiu
author_facet	Mingmei Chi Xiaoli Su Xiaojiao Sun Yan Xu Xiaoxia Wang Yanling Qiu
author_sort	Mingmei Chi
title	Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
title_short	Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
title_full	Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
title_fullStr	Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
title_full_unstemmed	Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS
title_sort	microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of aqds
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/216d443c5fc74706ab7c972140a4911b
work_keys_str_mv	AT mingmeichi microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds AT xiaolisu microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds AT xiaojiaosun microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds AT yanxu microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds AT xiaoxiawang microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds AT yanlingqiu microbialanalysisandenrichmentofanaerobicphenolandpcresoldegradingconsortiawithadditionofaqds
_version_	1718443718745260032

Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS

Ejemplares similares