The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics.
Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven...
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2021
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oai:doaj.org-article:216f3b91cf2b4236b9887c22b36649892021-12-02T20:02:54ZThe blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics.1553-73901553-740410.1371/journal.pgen.1009690https://doaj.org/article/216f3b91cf2b4236b9887c22b36649892021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009690https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven by endothelial cell (EC) rearrangement, which involves EC nucleus migration, junction remodeling, and actin cytoskeleton remodeling. Further observation reveals a growing difference in blood flow velocity between the two vessels in CV pruning in zebrafish embryos. With this model, we identify the critical role of Kruppel-like factor 6a (klf6a) in CV pruning. Disruption of klf6a functioning impairs CV pruning in zebrafish. klf6a is required for EC nucleus migration, junction remodeling, and actin cytoskeleton dynamics in zebrafish embryos. Moreover, actin-related protein transgelin 2 (tagln2) is a direct downstream target of klf6a in CV pruning in zebrafish embryos. Together these results demonstrate that the klf6a-tagln2 axis regulates CV pruning by promoting EC rearrangement.Lin WenTao ZhangJinxuan WangXuepu JinMuhammad Abdul RoufDesha LuoYuan ZhuDaoxi LeiHans GregersenYeqi WangGuixue WangPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009690 (2021) |
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DOAJ |
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DOAJ |
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Genetics QH426-470 |
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Genetics QH426-470 Lin Wen Tao Zhang Jinxuan Wang Xuepu Jin Muhammad Abdul Rouf Desha Luo Yuan Zhu Daoxi Lei Hans Gregersen Yeqi Wang Guixue Wang The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| description |
Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven by endothelial cell (EC) rearrangement, which involves EC nucleus migration, junction remodeling, and actin cytoskeleton remodeling. Further observation reveals a growing difference in blood flow velocity between the two vessels in CV pruning in zebrafish embryos. With this model, we identify the critical role of Kruppel-like factor 6a (klf6a) in CV pruning. Disruption of klf6a functioning impairs CV pruning in zebrafish. klf6a is required for EC nucleus migration, junction remodeling, and actin cytoskeleton dynamics in zebrafish embryos. Moreover, actin-related protein transgelin 2 (tagln2) is a direct downstream target of klf6a in CV pruning in zebrafish embryos. Together these results demonstrate that the klf6a-tagln2 axis regulates CV pruning by promoting EC rearrangement. |
| format |
article |
| author |
Lin Wen Tao Zhang Jinxuan Wang Xuepu Jin Muhammad Abdul Rouf Desha Luo Yuan Zhu Daoxi Lei Hans Gregersen Yeqi Wang Guixue Wang |
| author_facet |
Lin Wen Tao Zhang Jinxuan Wang Xuepu Jin Muhammad Abdul Rouf Desha Luo Yuan Zhu Daoxi Lei Hans Gregersen Yeqi Wang Guixue Wang |
| author_sort |
Lin Wen |
| title |
The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| title_short |
The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| title_full |
The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| title_fullStr |
The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| title_full_unstemmed |
The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| title_sort |
blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/216f3b91cf2b4236b9887c22b3664989 |
| work_keys_str_mv |
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