Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk

Summary: Bifidobacterium bifidum YIT 10347 (BF-1) is adhesive in vitro. Here we studied the molecular aspects of the BF-1 adhesion process. We identified and characterized non-adhesive mutants and found that a class E housekeeping sortase was critical for the adhesion to mucin. These mutants were si...

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Autores principales:	Eiji Ishikawa, Tetsuya Yamada, Kazuaki Yamaji, Masaki Serata, Daichi Fujii, Yoshinori Umesaki, Hirokazu Tsuji, Koji Nomoto, Masahiro Ito, Nobuhiko Okada, Masato Nagaoka, Atsushi Gomi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microbiology Microbiome Evolutionary ecology Science Q
Acceso en línea:	https://doaj.org/article/2171ab2f94a144d7b1c4cf1878eceb40
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spelling	oai:doaj.org-article:2171ab2f94a144d7b1c4cf1878eceb402021-11-20T05:10:41ZCritical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk2589-004210.1016/j.isci.2021.103363https://doaj.org/article/2171ab2f94a144d7b1c4cf1878eceb402021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013341https://doaj.org/toc/2589-0042Summary: Bifidobacterium bifidum YIT 10347 (BF-1) is adhesive in vitro. Here we studied the molecular aspects of the BF-1 adhesion process. We identified and characterized non-adhesive mutants and found that a class E housekeeping sortase was critical for the adhesion to mucin. These mutants were significantly less adhesive to GCIY cells than was the wild type (WT), which protected GCIY cells against acid treatment more than did a non-adhesive mutant. The non-adhesive mutants aberrantly accumulated precursors of putative sortase-dependent proteins (SDPs). Recombinant SDPs bound to mucin. Disruption of the housekeeping sortase influenced expression of SDPs and pilus components. Mutants defective in a pilin or in an SDP showed the same adhesion properties as WT. Therefore, multiple SDPs and pili seem to work cooperatively to achieve adhesion, and the housekeeping sortase is responsible for cell wall anchoring of its substrates to ensure their proper biological function.Eiji IshikawaTetsuya YamadaKazuaki YamajiMasaki SerataDaichi FujiiYoshinori UmesakiHirokazu TsujiKoji NomotoMasahiro ItoNobuhiko OkadaMasato NagaokaAtsushi GomiElsevierarticleMicrobiologyMicrobiomeEvolutionary ecologyScienceQENiScience, Vol 24, Iss 11, Pp 103363- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology Microbiome Evolutionary ecology Science Q
spellingShingle	Microbiology Microbiome Evolutionary ecology Science Q Eiji Ishikawa Tetsuya Yamada Kazuaki Yamaji Masaki Serata Daichi Fujii Yoshinori Umesaki Hirokazu Tsuji Koji Nomoto Masahiro Ito Nobuhiko Okada Masato Nagaoka Atsushi Gomi Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
description	Summary: Bifidobacterium bifidum YIT 10347 (BF-1) is adhesive in vitro. Here we studied the molecular aspects of the BF-1 adhesion process. We identified and characterized non-adhesive mutants and found that a class E housekeeping sortase was critical for the adhesion to mucin. These mutants were significantly less adhesive to GCIY cells than was the wild type (WT), which protected GCIY cells against acid treatment more than did a non-adhesive mutant. The non-adhesive mutants aberrantly accumulated precursors of putative sortase-dependent proteins (SDPs). Recombinant SDPs bound to mucin. Disruption of the housekeeping sortase influenced expression of SDPs and pilus components. Mutants defective in a pilin or in an SDP showed the same adhesion properties as WT. Therefore, multiple SDPs and pili seem to work cooperatively to achieve adhesion, and the housekeeping sortase is responsible for cell wall anchoring of its substrates to ensure their proper biological function.
format	article
author	Eiji Ishikawa Tetsuya Yamada Kazuaki Yamaji Masaki Serata Daichi Fujii Yoshinori Umesaki Hirokazu Tsuji Koji Nomoto Masahiro Ito Nobuhiko Okada Masato Nagaoka Atsushi Gomi
author_facet	Eiji Ishikawa Tetsuya Yamada Kazuaki Yamaji Masaki Serata Daichi Fujii Yoshinori Umesaki Hirokazu Tsuji Koji Nomoto Masahiro Ito Nobuhiko Okada Masato Nagaoka Atsushi Gomi
author_sort	Eiji Ishikawa
title	Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
title_short	Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
title_full	Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
title_fullStr	Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
title_full_unstemmed	Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
title_sort	critical roles of a housekeeping sortase of probiotic bifidobacterium bifidum in bacterium–host cell crosstalk
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2171ab2f94a144d7b1c4cf1878eceb40
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Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk

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