The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation

The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation

Thomas Mauger, Eric QuartettiHavener Eye Institute, Department of Ophthalmology, The Ohio State University, OH, USAPurpose: To analyze the levels of ß-glucuronidase during prolonged (14 day) corneal preservation with epithelialized (EPI) and deepithelialized (DEP) corneas and the effec...

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Autores principales: Mauger TF, Quartetti E
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
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Ophthalmology
RE1-994
Acceso en línea:https://doaj.org/article/217b4ba5b8d046f499200b15f48f1334
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spelling oai:doaj.org-article:217b4ba5b8d046f499200b15f48f13342021-12-02T02:53:16ZThe effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation1177-54671177-5483https://doaj.org/article/217b4ba5b8d046f499200b15f48f13342011-03-01T00:00:00Zhttp://www.dovepress.com/the-effects-of-the-presence-of-the-corneal-epithelium-and-supplemental-a6879https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Thomas Mauger, Eric QuartettiHavener Eye Institute, Department of Ophthalmology, The Ohio State University, OH, USAPurpose: To analyze the levels of ß-glucuronidase during prolonged (14 day) corneal preservation with epithelialized (EPI) and deepithelialized (DEP) corneas and the effect of supplemental hydrocortisone (HCT) on these levels.Methods: Thirty-six freshly excised bovine corneas were preserved in Optisol solution (4°C) for 14 days with the following conditions EPI/no supplemental HCT, DEP no supplemental HCT, EPI/10-8 M HCT, and EPI 10-4 M HCT. ß-glucuronidase activity levels were measured at the end of this period.Results: ß-glucuronidase levels (nmol/mL/h) for each group were found to be: EPI – No HCT: 4.302, SEM 0.586; DEP – No HCT: 2.178, SEM 0.271; EPI – 10-8 M HCT: 4.472, SEM 0.435; EPI – 10-4 M HCT: 2.072, SEM 0.437. The EPI – No HCT and EPI – 10-8 M HCT were not significantly different as were the DEP – No HCT and EPI – 10-4 M HCT groups. The EPI – No HCT group and the EPI – 10-8 M HCT group were independently significantly different from the DEP – No HCT and the EPI – 10-4 M HCT groups.Conclusions: The corneal epithelium contributes significantly to the formation of the lysosomal enzyme, ß-glucuronidase, during preservation. The addition of 10-4 M HCT decreases the production of ß-glucuronidase during corneal preservation in this model.Keywords: ß-glucuronidase, preservation  Mauger TFQuartetti EDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2011, Iss default, Pp 415-417 (2011)
institution DOAJ
collection DOAJ
language EN
topic Ophthalmology
RE1-994
spellingShingle Ophthalmology
RE1-994
Mauger TF
Quartetti E
The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
description Thomas Mauger, Eric QuartettiHavener Eye Institute, Department of Ophthalmology, The Ohio State University, OH, USAPurpose: To analyze the levels of ß-glucuronidase during prolonged (14 day) corneal preservation with epithelialized (EPI) and deepithelialized (DEP) corneas and the effect of supplemental hydrocortisone (HCT) on these levels.Methods: Thirty-six freshly excised bovine corneas were preserved in Optisol solution (4°C) for 14 days with the following conditions EPI/no supplemental HCT, DEP no supplemental HCT, EPI/10-8 M HCT, and EPI 10-4 M HCT. ß-glucuronidase activity levels were measured at the end of this period.Results: ß-glucuronidase levels (nmol/mL/h) for each group were found to be: EPI – No HCT: 4.302, SEM 0.586; DEP – No HCT: 2.178, SEM 0.271; EPI – 10-8 M HCT: 4.472, SEM 0.435; EPI – 10-4 M HCT: 2.072, SEM 0.437. The EPI – No HCT and EPI – 10-8 M HCT were not significantly different as were the DEP – No HCT and EPI – 10-4 M HCT groups. The EPI – No HCT group and the EPI – 10-8 M HCT group were independently significantly different from the DEP – No HCT and the EPI – 10-4 M HCT groups.Conclusions: The corneal epithelium contributes significantly to the formation of the lysosomal enzyme, ß-glucuronidase, during preservation. The addition of 10-4 M HCT decreases the production of ß-glucuronidase during corneal preservation in this model.Keywords: ß-glucuronidase, preservation 
format article
author Mauger TF
Quartetti E
author_facet Mauger TF
Quartetti E
author_sort Mauger TF
title The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
title_short The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
title_full The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
title_fullStr The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
title_full_unstemmed The effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
title_sort effects of the presence of the corneal epithelium and supplemental hydrocortisone on ß-glucuronidase levels with corneal preservation
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/217b4ba5b8d046f499200b15f48f1334
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