Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2

Ai-Zheng Chen,1,2 Guang-Ya Wang,1 Shi-Bin Wang,1,2 Li Li,1 Yuan-Gang Liu,1,2 Chen Zhao11College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, ChinaBackground: The aim of this study was to improv...

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Autores principales:	Chen AZ, Wang GY, Wang SB, Li L, Liu YG, Zhao C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/217c8c6b1ac2481b8c4e2c84583d7835
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spelling	oai:doaj.org-article:217c8c6b1ac2481b8c4e2c84583d78352021-12-02T02:30:07ZFormation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO21176-91141178-2013https://doaj.org/article/217c8c6b1ac2481b8c4e2c84583d78352012-06-01T00:00:00Zhttp://www.dovepress.com/formation-of-methotrexate-plla-peg-plla-composite-microspheres-by-micr-a10140https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ai-Zheng Chen,1,2 Guang-Ya Wang,1 Shi-Bin Wang,1,2 Li Li,1 Yuan-Gang Liu,1,2 Chen Zhao11College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, ChinaBackground: The aim of this study was to improve the drug loading, encapsulation efficiency, and sustained-release properties of supercritical CO2-based drug-loaded polymer carriers via a process of suspension-enhanced dispersion by supercritical CO2 (SpEDS), which is an advanced version of solution-enhanced dispersion by supercritical CO2 (SEDS).Methods: Methotrexate nanoparticles were successfully microencapsulated into poly (L-lactide)-poly(ethylene glycol)-poly(L-lactide) (PLLA-PEG-PLLA) by SpEDS. Methotrexate nanoparticles were first prepared by SEDS, then suspended in PLLA-PEG-PLLA solution, and finally microencapsulated into PLLA-PEG-PLLA via SpEDS, where an &quot;injector&quot; was utilized in the suspension delivery system.Results: After microencapsulation, the composite methotrexate (MTX)-PLLA-PEG-PLLA microspheres obtained had a mean particle size of 545 nm, drug loading of 13.7%, and an encapsulation efficiency of 39.2%. After an initial burst release, with around 65% of the total methotrexate being released in the first 3 hours, the MTX-PLLA-PEG-PLLA microspheres released methotrexate in a sustained manner, with 85% of the total methotrexate dose released within 23 hours and nearly 100% within 144 hours.Conclusion: Compared with a parallel study of the coprecipitation process, microencapsulation using SpEDS offered greater potential to manufacture drug-loaded polymer microspheres for a drug delivery system.Keywords: drug loading, encapsulation efficiency, methotrexate, nanoparticles, poly(L-lactide), supercritical CO2 sustained releaseChen AZWang GYWang SBLi LLiu YGZhao CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3013-3022 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen AZ Wang GY Wang SB Li L Liu YG Zhao C Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
description	Ai-Zheng Chen,1,2 Guang-Ya Wang,1 Shi-Bin Wang,1,2 Li Li,1 Yuan-Gang Liu,1,2 Chen Zhao11College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, ChinaBackground: The aim of this study was to improve the drug loading, encapsulation efficiency, and sustained-release properties of supercritical CO2-based drug-loaded polymer carriers via a process of suspension-enhanced dispersion by supercritical CO2 (SpEDS), which is an advanced version of solution-enhanced dispersion by supercritical CO2 (SEDS).Methods: Methotrexate nanoparticles were successfully microencapsulated into poly (L-lactide)-poly(ethylene glycol)-poly(L-lactide) (PLLA-PEG-PLLA) by SpEDS. Methotrexate nanoparticles were first prepared by SEDS, then suspended in PLLA-PEG-PLLA solution, and finally microencapsulated into PLLA-PEG-PLLA via SpEDS, where an &quot;injector&quot; was utilized in the suspension delivery system.Results: After microencapsulation, the composite methotrexate (MTX)-PLLA-PEG-PLLA microspheres obtained had a mean particle size of 545 nm, drug loading of 13.7%, and an encapsulation efficiency of 39.2%. After an initial burst release, with around 65% of the total methotrexate being released in the first 3 hours, the MTX-PLLA-PEG-PLLA microspheres released methotrexate in a sustained manner, with 85% of the total methotrexate dose released within 23 hours and nearly 100% within 144 hours.Conclusion: Compared with a parallel study of the coprecipitation process, microencapsulation using SpEDS offered greater potential to manufacture drug-loaded polymer microspheres for a drug delivery system.Keywords: drug loading, encapsulation efficiency, methotrexate, nanoparticles, poly(L-lactide), supercritical CO2 sustained release
format	article
author	Chen AZ Wang GY Wang SB Li L Liu YG Zhao C
author_facet	Chen AZ Wang GY Wang SB Li L Liu YG Zhao C
author_sort	Chen AZ
title	Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
title_short	Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
title_full	Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
title_fullStr	Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
title_full_unstemmed	Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2
title_sort	formation of methotrexate-plla-peg-plla composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical co2
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/217c8c6b1ac2481b8c4e2c84583d7835
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Formation of methotrexate-PLLA-PEG-PLLA composite microspheres by microencapsulation through a process of suspension-enhanced dispersion by supercritical CO2

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