Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and stands among the leading causes of cancer-related deaths. Although deregulation of the microbiota in the gastrointestinal tract has been frequently described in CRC, very little is known about the precise molecular...

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Autores principales:	Xie Xiaoliang, Jiang Dan, Zhou Xuebing, Ye Xiaoping, Yang Ping, He Yaqin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	recombinant bacteroides fragilis enterotoxin-1 colorectal cancer chemokine c–c motif ligand 3 nf-κb Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/217fd4688cca41f789e6bb2797f26d24
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spelling	oai:doaj.org-article:217fd4688cca41f789e6bb2797f26d242021-12-05T14:10:41ZRecombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways2391-541210.1515/biol-2021-0043https://doaj.org/article/217fd4688cca41f789e6bb2797f26d242021-04-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0043https://doaj.org/toc/2391-5412Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and stands among the leading causes of cancer-related deaths. Although deregulation of the microbiota in the gastrointestinal tract has been frequently described in CRC, very little is known about the precise molecular mechanisms by which bacteria and their toxins modulate the process of tumorigenesis and behavior of cancer cells. In this study, we produced recombinant Bacteroides fragilis enterotoxin-1 (rBFT1) and demonstrate that rBFT1 could promote cell proliferation in colorectal cancer cells and accelerate tumor growth in vivo. To identify the mechanisms, we further investigated CCL3/CCR5 and NF-κB pathway. We found that CCL3, CCR5, NF-κB, and TRAF-6 were dramatically upregulated after rBFT1 treatment, thus suggesting that the role of rBFT1 in CRC progression may be associated with CCL3/CCR5 and NF-κB pathways. Collectively, our results indicate that rBFT1 serves as a tumor promoter and plays a crucial role in inducing the proliferation of CRC via accelerating CCL3-related molecular pathway, thus giving insights into mechanistic underpinnings for the prevention and treatment of CRC.Xie XiaoliangJiang DanZhou XuebingYe XiaopingYang PingHe YaqinDe Gruyterarticlerecombinant bacteroides fragilis enterotoxin-1colorectal cancerchemokine c–c motif ligand 3nf-κbBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 408-418 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	recombinant bacteroides fragilis enterotoxin-1 colorectal cancer chemokine c–c motif ligand 3 nf-κb Biology (General) QH301-705.5
spellingShingle	recombinant bacteroides fragilis enterotoxin-1 colorectal cancer chemokine c–c motif ligand 3 nf-κb Biology (General) QH301-705.5 Xie Xiaoliang Jiang Dan Zhou Xuebing Ye Xiaoping Yang Ping He Yaqin Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
description	Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and stands among the leading causes of cancer-related deaths. Although deregulation of the microbiota in the gastrointestinal tract has been frequently described in CRC, very little is known about the precise molecular mechanisms by which bacteria and their toxins modulate the process of tumorigenesis and behavior of cancer cells. In this study, we produced recombinant Bacteroides fragilis enterotoxin-1 (rBFT1) and demonstrate that rBFT1 could promote cell proliferation in colorectal cancer cells and accelerate tumor growth in vivo. To identify the mechanisms, we further investigated CCL3/CCR5 and NF-κB pathway. We found that CCL3, CCR5, NF-κB, and TRAF-6 were dramatically upregulated after rBFT1 treatment, thus suggesting that the role of rBFT1 in CRC progression may be associated with CCL3/CCR5 and NF-κB pathways. Collectively, our results indicate that rBFT1 serves as a tumor promoter and plays a crucial role in inducing the proliferation of CRC via accelerating CCL3-related molecular pathway, thus giving insights into mechanistic underpinnings for the prevention and treatment of CRC.
format	article
author	Xie Xiaoliang Jiang Dan Zhou Xuebing Ye Xiaoping Yang Ping He Yaqin
author_facet	Xie Xiaoliang Jiang Dan Zhou Xuebing Ye Xiaoping Yang Ping He Yaqin
author_sort	Xie Xiaoliang
title	Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
title_short	Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
title_full	Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
title_fullStr	Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
title_full_unstemmed	Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways
title_sort	recombinant bacteroides fragilis enterotoxin-1 (rbft-1) promotes proliferation of colorectal cancer via ccl3-related molecular pathways
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/217fd4688cca41f789e6bb2797f26d24
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Recombinant Bacteroides fragilis enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways

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