Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD

Abstract Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively control...

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Autores principales:	Le Zhang, Joost Willemse, Paul A. Hoskisson, Gilles P. van Wezel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/218da1ea068b4ace80586f42c76fa79d
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spelling	oai:doaj.org-article:218da1ea068b4ace80586f42c76fa79d2021-12-02T16:07:50ZSporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD10.1038/s41598-018-25782-12045-2322https://doaj.org/article/218da1ea068b4ace80586f42c76fa79d2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25782-1https://doaj.org/toc/2045-2322Abstract Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor. Electron and fluorescence microscopy demonstrated that ylmE mutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed in ylmE mutants. Deletion of ylmD had a far less severe effect on sporulation. Interestingly, the additional deletion of ylmD restored sporulation to the ylmE null mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division in S. coelicolor, whereby the presence of YlmD alone results in major developmental defects.Le ZhangJoost WillemsePaul A. HoskissonGilles P. van WezelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Le Zhang Joost Willemse Paul A. Hoskisson Gilles P. van Wezel Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
description	Abstract Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor. Electron and fluorescence microscopy demonstrated that ylmE mutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed in ylmE mutants. Deletion of ylmD had a far less severe effect on sporulation. Interestingly, the additional deletion of ylmD restored sporulation to the ylmE null mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division in S. coelicolor, whereby the presence of YlmD alone results in major developmental defects.
format	article
author	Le Zhang Joost Willemse Paul A. Hoskisson Gilles P. van Wezel
author_facet	Le Zhang Joost Willemse Paul A. Hoskisson Gilles P. van Wezel
author_sort	Le Zhang
title	Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
title_short	Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
title_full	Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
title_fullStr	Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
title_full_unstemmed	Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
title_sort	sporulation-specific cell division defects in ylme mutants of streptomyces coelicolor are rescued by additional deletion of ylmd
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/218da1ea068b4ace80586f42c76fa79d
work_keys_str_mv	AT lezhang sporulationspecificcelldivisiondefectsinylmemutantsofstreptomycescoelicolorarerescuedbyadditionaldeletionofylmd AT joostwillemse sporulationspecificcelldivisiondefectsinylmemutantsofstreptomycescoelicolorarerescuedbyadditionaldeletionofylmd AT paulahoskisson sporulationspecificcelldivisiondefectsinylmemutantsofstreptomycescoelicolorarerescuedbyadditionaldeletionofylmd AT gillespvanwezel sporulationspecificcelldivisiondefectsinylmemutantsofstreptomycescoelicolorarerescuedbyadditionaldeletionofylmd
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Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD

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