Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)

Kiwifruit (Actinidia eriantha) is a peculiar berry resource in China, and the maturation period is generally late. Fortunately, we found an early mature A. eriantha germplasm. In order to explore the formation mechanism of its early mature trait, we determined the main carbohydrate and endogenous ho...

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Autores principales:	Guanglian Liao, Qing Liu, Xiaobiao Xu, Yanqun He, Yiqi Li, Hailing Wang, Bin Ye, Chunhui Huang, Min Zhong, Dongfeng Jia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Actinidia eriantha early mature trait non-target metabolomics sucrose metabolism transcriptomics resequencing Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/218f0eecc43f4080808165f39647561e
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spelling	oai:doaj.org-article:218f0eecc43f4080808165f39647561e2021-11-19T07:39:12ZMetabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)1664-462X10.3389/fpls.2021.760496https://doaj.org/article/218f0eecc43f4080808165f39647561e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.760496/fullhttps://doaj.org/toc/1664-462XKiwifruit (Actinidia eriantha) is a peculiar berry resource in China, and the maturation period is generally late. Fortunately, we found an early mature A. eriantha germplasm. In order to explore the formation mechanism of its early mature trait, we determined the main carbohydrate and endogenous hormone content of the fruit, and used off-target metabolomics and transcriptomics to identify key regulatory metabolites and genes. We found that early mature germplasm had faster starch conversion rate and higher sucrose, glucose, and fructose content when harvested, while with lower auxin (IAA), abscisic acid (ABA), and zeatin (ZR) content. Through the non-targeted metabolome, 19 and 20 metabolites closely related to fruit maturity and early maturity were identified, respectively. At the same time, weighted correlation network analysis (WGCNA) showed that these metabolites were regulated by 73 and 99 genes, respectively, especially genes related to sugar metabolism were mostly. Based on above, the formation of early mature trait of A. eriantha was mainly due to the sucrose decomposition rate was reduced and the soluble solid content (SSC) accumulated at low levels of endogenous hormones, so as to reach the harvest standard earlier than the late mature germplasm. Finally, ten single nucleotide polymorphism (SNP) loci were developed which can be used for the identification of early mature trait of A. eriantha.Guanglian LiaoGuanglian LiaoQing LiuXiaobiao XuXiaobiao XuYanqun HeYiqi LiHailing WangBin YeChunhui HuangMin ZhongDongfeng JiaFrontiers Media S.A.articleActinidia erianthaearly mature traitnon-target metabolomicssucrose metabolismtranscriptomicsresequencingPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Actinidia eriantha early mature trait non-target metabolomics sucrose metabolism transcriptomics resequencing Plant culture SB1-1110
spellingShingle	Actinidia eriantha early mature trait non-target metabolomics sucrose metabolism transcriptomics resequencing Plant culture SB1-1110 Guanglian Liao Guanglian Liao Qing Liu Xiaobiao Xu Xiaobiao Xu Yanqun He Yiqi Li Hailing Wang Bin Ye Chunhui Huang Min Zhong Dongfeng Jia Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
description	Kiwifruit (Actinidia eriantha) is a peculiar berry resource in China, and the maturation period is generally late. Fortunately, we found an early mature A. eriantha germplasm. In order to explore the formation mechanism of its early mature trait, we determined the main carbohydrate and endogenous hormone content of the fruit, and used off-target metabolomics and transcriptomics to identify key regulatory metabolites and genes. We found that early mature germplasm had faster starch conversion rate and higher sucrose, glucose, and fructose content when harvested, while with lower auxin (IAA), abscisic acid (ABA), and zeatin (ZR) content. Through the non-targeted metabolome, 19 and 20 metabolites closely related to fruit maturity and early maturity were identified, respectively. At the same time, weighted correlation network analysis (WGCNA) showed that these metabolites were regulated by 73 and 99 genes, respectively, especially genes related to sugar metabolism were mostly. Based on above, the formation of early mature trait of A. eriantha was mainly due to the sucrose decomposition rate was reduced and the soluble solid content (SSC) accumulated at low levels of endogenous hormones, so as to reach the harvest standard earlier than the late mature germplasm. Finally, ten single nucleotide polymorphism (SNP) loci were developed which can be used for the identification of early mature trait of A. eriantha.
format	article
author	Guanglian Liao Guanglian Liao Qing Liu Xiaobiao Xu Xiaobiao Xu Yanqun He Yiqi Li Hailing Wang Bin Ye Chunhui Huang Min Zhong Dongfeng Jia
author_facet	Guanglian Liao Guanglian Liao Qing Liu Xiaobiao Xu Xiaobiao Xu Yanqun He Yiqi Li Hailing Wang Bin Ye Chunhui Huang Min Zhong Dongfeng Jia
author_sort	Guanglian Liao
title	Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
title_short	Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
title_full	Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
title_fullStr	Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
title_full_unstemmed	Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)
title_sort	metabolome and transcriptome reveal novel formation mechanism of early mature trait in kiwifruit (actinidia eriantha)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/218f0eecc43f4080808165f39647561e
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Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)

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