Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.

In Rhizobia the Irr protein is an important regulator for iron-dependent gene expression. We studied the role of the Irr homolog RSP_3179 in the photosynthetic alpha-proteobacterium Rhodobacter sphaeroides. While Irr had little effect on growth under iron-limiting or non-limiting conditions its dele...

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Autores principales:	Verena Peuser, Bernhard Remes, Gabriele Klug
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/218f23cdd3494e228db72f22a5354a20
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spelling	oai:doaj.org-article:218f23cdd3494e228db72f22a5354a202021-11-18T07:09:27ZRole of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.1932-620310.1371/journal.pone.0042231https://doaj.org/article/218f23cdd3494e228db72f22a5354a202012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879920/?tool=EBIhttps://doaj.org/toc/1932-6203In Rhizobia the Irr protein is an important regulator for iron-dependent gene expression. We studied the role of the Irr homolog RSP_3179 in the photosynthetic alpha-proteobacterium Rhodobacter sphaeroides. While Irr had little effect on growth under iron-limiting or non-limiting conditions its deletion resulted in increased resistance to hydrogen peroxide and singlet oxygen. This correlates with an elevated expression of katE for catalase in the Irr mutant compared to the wild type under non-stress conditions. Transcriptome studies revealed that Irr affects the expression of genes for iron metabolism, but also has some influence on genes involved in stress response, citric acid cycle, oxidative phosphorylation, transport, and photosynthesis. Most genes showed higher expression levels in the wild type than in the mutant under normal growth conditions indicating an activator function of Irr. Irr was however not required to activate genes of the iron metabolism in response to iron limitation, which showed even stronger induction in the absence of Irr. This was also true for genes mbfA and ccpA, which were verified as direct targets for Irr. Our results suggest that in R. sphaeroides Irr diminishes the strong induction of genes for iron metabolism under iron starvation.Verena PeuserBernhard RemesGabriele KlugPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42231 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Verena Peuser Bernhard Remes Gabriele Klug Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
description	In Rhizobia the Irr protein is an important regulator for iron-dependent gene expression. We studied the role of the Irr homolog RSP_3179 in the photosynthetic alpha-proteobacterium Rhodobacter sphaeroides. While Irr had little effect on growth under iron-limiting or non-limiting conditions its deletion resulted in increased resistance to hydrogen peroxide and singlet oxygen. This correlates with an elevated expression of katE for catalase in the Irr mutant compared to the wild type under non-stress conditions. Transcriptome studies revealed that Irr affects the expression of genes for iron metabolism, but also has some influence on genes involved in stress response, citric acid cycle, oxidative phosphorylation, transport, and photosynthesis. Most genes showed higher expression levels in the wild type than in the mutant under normal growth conditions indicating an activator function of Irr. Irr was however not required to activate genes of the iron metabolism in response to iron limitation, which showed even stronger induction in the absence of Irr. This was also true for genes mbfA and ccpA, which were verified as direct targets for Irr. Our results suggest that in R. sphaeroides Irr diminishes the strong induction of genes for iron metabolism under iron starvation.
format	article
author	Verena Peuser Bernhard Remes Gabriele Klug
author_facet	Verena Peuser Bernhard Remes Gabriele Klug
author_sort	Verena Peuser
title	Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
title_short	Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
title_full	Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
title_fullStr	Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
title_full_unstemmed	Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.
title_sort	role of the irr protein in the regulation of iron metabolism in rhodobacter sphaeroides.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/218f23cdd3494e228db72f22a5354a20
work_keys_str_mv	AT verenapeuser roleoftheirrproteinintheregulationofironmetabolisminrhodobactersphaeroides AT bernhardremes roleoftheirrproteinintheregulationofironmetabolisminrhodobactersphaeroides AT gabrieleklug roleoftheirrproteinintheregulationofironmetabolisminrhodobactersphaeroides
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Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.

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