Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade

Recurrent pregnancy loss (RPL) is closely associated with insufficient functions of trophoblastic cells. Circular RNA forkhead box P1 (circFOXP1) can regulate cell activities in different types of diseases. However, its effects on trophoblastic cells and its role in RPL development remain unknown. I...

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Autores principales:	Yuan Gao, Yukun Tang, Qian Sun, Guixue Guan, Xiaoyan Wu, Fan Shi, Zihao Zhou, Wen Yang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	circfoxp1 mir–143–3p s100a11 recurrent pregnancy loss epithelial-to-mesenchymal transition Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2195b49076a2444497ced6d650d3025c
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spelling	oai:doaj.org-article:2195b49076a2444497ced6d650d3025c2021-11-04T15:51:53ZCircular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade2165-59792165-598710.1080/21655979.2021.1988374https://doaj.org/article/2195b49076a2444497ced6d650d3025c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1988374https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Recurrent pregnancy loss (RPL) is closely associated with insufficient functions of trophoblastic cells. Circular RNA forkhead box P1 (circFOXP1) can regulate cell activities in different types of diseases. However, its effects on trophoblastic cells and its role in RPL development remain unknown. In this study, gene expressions were detected by RT-qPCR. Protein levels were detected by Western blotting. Trophoblastic cell viability, apoptosis, invasion, and migration were respectively analyzed via CCK-8, flow cytometry, wound healing, and transwell assays. The association between miR–143–3p and circFOXP1 or S100A11 (S100 calcium binding protein A11) was explored and confirmed by bioinformatics prediction and luciferase reporter assay. Herein, miR–143–3p was upregulated in RPL. Furthermore, miR–143–3p upregulation induced apoptosis and suppressed proliferation, epithelial-to-mesenchymal transition (EMT) process, and metastatic capabilities of trophoblastic cells; whereas, miR–143–3p inhibition exert opposite effects. MiR–143–3p targeted S100A11 and was adversely regulated by circFOXP1 expression. S100A11 inhibition partially offset the effect of miR–143–3p knockdown on trophoblastic cell viability, apoptosis, EMT, invasion, and migration. In addition, circFOXP1 competitively combined with miR–143–3p, thus regulating S100A11 expression. Moreover, circFOXP1 regulated trophoblastic cell functions through the miR–143–3p/S100A11 cascade. To sum up, our study, for the first time, demonstrated that circFOXP1 could improve dysfunction of trophoblastic cells through the miR–143–3p/S100A11 axis, providing novel biomarkers and diagnostic targets for RPL.Yuan GaoYukun TangQian SunGuixue GuanXiaoyan WuFan ShiZihao ZhouWen YangTaylor & Francis Grouparticlecircfoxp1mir–143–3ps100a11recurrent pregnancy lossepithelial-to-mesenchymal transitionBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 9081-9093 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circfoxp1 mir–143–3p s100a11 recurrent pregnancy loss epithelial-to-mesenchymal transition Biotechnology TP248.13-248.65
spellingShingle	circfoxp1 mir–143–3p s100a11 recurrent pregnancy loss epithelial-to-mesenchymal transition Biotechnology TP248.13-248.65 Yuan Gao Yukun Tang Qian Sun Guixue Guan Xiaoyan Wu Fan Shi Zihao Zhou Wen Yang Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
description	Recurrent pregnancy loss (RPL) is closely associated with insufficient functions of trophoblastic cells. Circular RNA forkhead box P1 (circFOXP1) can regulate cell activities in different types of diseases. However, its effects on trophoblastic cells and its role in RPL development remain unknown. In this study, gene expressions were detected by RT-qPCR. Protein levels were detected by Western blotting. Trophoblastic cell viability, apoptosis, invasion, and migration were respectively analyzed via CCK-8, flow cytometry, wound healing, and transwell assays. The association between miR–143–3p and circFOXP1 or S100A11 (S100 calcium binding protein A11) was explored and confirmed by bioinformatics prediction and luciferase reporter assay. Herein, miR–143–3p was upregulated in RPL. Furthermore, miR–143–3p upregulation induced apoptosis and suppressed proliferation, epithelial-to-mesenchymal transition (EMT) process, and metastatic capabilities of trophoblastic cells; whereas, miR–143–3p inhibition exert opposite effects. MiR–143–3p targeted S100A11 and was adversely regulated by circFOXP1 expression. S100A11 inhibition partially offset the effect of miR–143–3p knockdown on trophoblastic cell viability, apoptosis, EMT, invasion, and migration. In addition, circFOXP1 competitively combined with miR–143–3p, thus regulating S100A11 expression. Moreover, circFOXP1 regulated trophoblastic cell functions through the miR–143–3p/S100A11 cascade. To sum up, our study, for the first time, demonstrated that circFOXP1 could improve dysfunction of trophoblastic cells through the miR–143–3p/S100A11 axis, providing novel biomarkers and diagnostic targets for RPL.
format	article
author	Yuan Gao Yukun Tang Qian Sun Guixue Guan Xiaoyan Wu Fan Shi Zihao Zhou Wen Yang
author_facet	Yuan Gao Yukun Tang Qian Sun Guixue Guan Xiaoyan Wu Fan Shi Zihao Zhou Wen Yang
author_sort	Yuan Gao
title	Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
title_short	Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
title_full	Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
title_fullStr	Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
title_full_unstemmed	Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade
title_sort	circular rna foxp1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the mir-143-3p/s100a11 cascade
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2195b49076a2444497ced6d650d3025c
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Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade

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