The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors

A balanced, diverse gut microbiota is vital for animal health. The microbial population is shaped by multiple factors including genetic background and environment, but other determinants remain controversial. Numerous studies suggest that the dominant factor is genetic background while others emphas...

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Autores principales:	Jiandong Yang, Yun Zhong, Liqun Xu, Bo Zeng, Kang Lai, Mingxian Yang, Diyan Li, Ye Zhao, Mingwang Zhang, Debing Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	honeybee queen gut microbiota amplicon sequencing asexual hybridization crossbreed Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/219bed6998384f69879266377ebe8234
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spelling	oai:doaj.org-article:219bed6998384f69879266377ebe82342021-11-05T16:21:04ZThe Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors1664-302X10.3389/fmicb.2021.722901https://doaj.org/article/219bed6998384f69879266377ebe82342021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.722901/fullhttps://doaj.org/toc/1664-302XA balanced, diverse gut microbiota is vital for animal health. The microbial population is shaped by multiple factors including genetic background and environment, but other determinants remain controversial. Numerous studies suggest that the dominant factor is genetic background while others emphasize the environmental factors. Here, we bred asexual hybridization queens (AHQs) of honeybees through nutritional crossbreeding (laid in Apis mellifera colony but bred in Apis cerana colony), sequenced their gut microbiome, and compared it with normally bred sister queens to determine the primary factor shaping the gut microbiota. Our results showed that the dominant genera in the gut microbiota of AHQs were Brevundimonas, Bombella, and Lactobacillus, and its microbial community was more related to A. mellifera queens. The AHQs had a moderate number of different bacterial species and diversity, but total bacterial numbers were low. There were more significant taxa identified in the comparison between AHQ and A. cerana queen according to LEfSe analysis results. The only genetic-specific taxon we figured out was Brevundimonas. The growth of core bacterial abundance showed different characteristics among different queen groups in the first week after emerging. Collectively, this study suggested that the genetic background played a more dominant role than environmental factors in shaping the gut microbiota of honeybee queen and the microbiota of midgut was more sensitive than that of rectum to this impact.Jiandong YangYun ZhongLiqun XuBo ZengKang LaiMingxian YangDiyan LiYe ZhaoMingwang ZhangDebing LiFrontiers Media S.A.articlehoneybeequeengut microbiotaamplicon sequencingasexual hybridizationcrossbreedMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	honeybee queen gut microbiota amplicon sequencing asexual hybridization crossbreed Microbiology QR1-502
spellingShingle	honeybee queen gut microbiota amplicon sequencing asexual hybridization crossbreed Microbiology QR1-502 Jiandong Yang Yun Zhong Liqun Xu Bo Zeng Kang Lai Mingxian Yang Diyan Li Ye Zhao Mingwang Zhang Debing Li The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
description	A balanced, diverse gut microbiota is vital for animal health. The microbial population is shaped by multiple factors including genetic background and environment, but other determinants remain controversial. Numerous studies suggest that the dominant factor is genetic background while others emphasize the environmental factors. Here, we bred asexual hybridization queens (AHQs) of honeybees through nutritional crossbreeding (laid in Apis mellifera colony but bred in Apis cerana colony), sequenced their gut microbiome, and compared it with normally bred sister queens to determine the primary factor shaping the gut microbiota. Our results showed that the dominant genera in the gut microbiota of AHQs were Brevundimonas, Bombella, and Lactobacillus, and its microbial community was more related to A. mellifera queens. The AHQs had a moderate number of different bacterial species and diversity, but total bacterial numbers were low. There were more significant taxa identified in the comparison between AHQ and A. cerana queen according to LEfSe analysis results. The only genetic-specific taxon we figured out was Brevundimonas. The growth of core bacterial abundance showed different characteristics among different queen groups in the first week after emerging. Collectively, this study suggested that the genetic background played a more dominant role than environmental factors in shaping the gut microbiota of honeybee queen and the microbiota of midgut was more sensitive than that of rectum to this impact.
format	article
author	Jiandong Yang Yun Zhong Liqun Xu Bo Zeng Kang Lai Mingxian Yang Diyan Li Ye Zhao Mingwang Zhang Debing Li
author_facet	Jiandong Yang Yun Zhong Liqun Xu Bo Zeng Kang Lai Mingxian Yang Diyan Li Ye Zhao Mingwang Zhang Debing Li
author_sort	Jiandong Yang
title	The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
title_short	The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
title_full	The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
title_fullStr	The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
title_full_unstemmed	The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors
title_sort	dominating role of genetic background in shaping gut microbiota of honeybee queen over environmental factors
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/219bed6998384f69879266377ebe8234
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The Dominating Role of Genetic Background in Shaping Gut Microbiota of Honeybee Queen Over Environmental Factors

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