Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal

Abstract The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fab...

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Autores principales: Ester Drastíková, Klára Konderlová, Andrea Šebestová, Daniel Baron, Petra Švecová, Petra Táborská, Kateřina Vítková, Veronika Pospíšilová, Serhiy Forostyak, Zdeněk Kořístek, Ludmila Porubová, Jan Petr
Formato: article
Lenguaje:EN
Publicado: SpringerOpen 2021
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Acceso en línea:https://doaj.org/article/219dd575a13b47ac9d114d207314f144
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Sumario:Abstract The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fabrication procedure. The one-step PDMS microdevices were found to be the best in the view of preparation, repeatability, and stability of the reagent. This design was then applied for the determination of total protein content in biomedical products using the Bradford assay.