Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal

Abstract The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fab...

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Autores principales:	Ester Drastíková, Klára Konderlová, Andrea Šebestová, Daniel Baron, Petra Švecová, Petra Táborská, Kateřina Vítková, Veronika Pospíšilová, Serhiy Forostyak, Zdeněk Kořístek, Ludmila Porubová, Jan Petr
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Lab-in-a-syringe 3D printing Microfluidics PDMS Total protein content Chemistry QD1-999 Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/219dd575a13b47ac9d114d207314f144
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spelling	oai:doaj.org-article:219dd575a13b47ac9d114d207314f1442021-11-28T12:06:08ZDetermination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal10.1186/s40543-021-00307-02093-3371https://doaj.org/article/219dd575a13b47ac9d114d207314f1442021-11-01T00:00:00Zhttps://doi.org/10.1186/s40543-021-00307-0https://doaj.org/toc/2093-3371Abstract The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fabrication procedure. The one-step PDMS microdevices were found to be the best in the view of preparation, repeatability, and stability of the reagent. This design was then applied for the determination of total protein content in biomedical products using the Bradford assay.Ester DrastíkováKlára KonderlováAndrea ŠebestováDaniel BaronPetra ŠvecováPetra TáborskáKateřina VítkováVeronika PospíšilováSerhiy ForostyakZdeněk KořístekLudmila PorubováJan PetrSpringerOpenarticleLab-in-a-syringe3D printingMicrofluidicsPDMSTotal protein contentChemistryQD1-999Analytical chemistryQD71-142ENJournal of Analytical Science and Technology, Vol 12, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lab-in-a-syringe 3D printing Microfluidics PDMS Total protein content Chemistry QD1-999 Analytical chemistry QD71-142
spellingShingle	Lab-in-a-syringe 3D printing Microfluidics PDMS Total protein content Chemistry QD1-999 Analytical chemistry QD71-142 Ester Drastíková Klára Konderlová Andrea Šebestová Daniel Baron Petra Švecová Petra Táborská Kateřina Vítková Veronika Pospíšilová Serhiy Forostyak Zdeněk Kořístek Ludmila Porubová Jan Petr Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
description	Abstract The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fabrication procedure. The one-step PDMS microdevices were found to be the best in the view of preparation, repeatability, and stability of the reagent. This design was then applied for the determination of total protein content in biomedical products using the Bradford assay.
format	article
author	Ester Drastíková Klára Konderlová Andrea Šebestová Daniel Baron Petra Švecová Petra Táborská Kateřina Vítková Veronika Pospíšilová Serhiy Forostyak Zdeněk Kořístek Ludmila Porubová Jan Petr
author_facet	Ester Drastíková Klára Konderlová Andrea Šebestová Daniel Baron Petra Švecová Petra Táborská Kateřina Vítková Veronika Pospíšilová Serhiy Forostyak Zdeněk Kořístek Ludmila Porubová Jan Petr
author_sort	Ester Drastíková
title	Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
title_short	Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
title_full	Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
title_fullStr	Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
title_full_unstemmed	Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal
title_sort	determination of total protein content in biomedical products by the pdms-assisted lab-in-a-syringe assay using 3d printed scaffolds removal
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/219dd575a13b47ac9d114d207314f144
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Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal

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