The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos
Abstract The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process. It expects to provide a theoretical basis for the application of micro...
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Nature Portfolio
2021
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oai:doaj.org-article:21a309039e904bc4b646178b22b5b84a2021-12-02T13:20:22ZThe effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos10.1038/s41598-021-84875-62045-2322https://doaj.org/article/21a309039e904bc4b646178b22b5b84a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84875-6https://doaj.org/toc/2045-2322Abstract The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process. It expects to provide a theoretical basis for the application of microbes in tobacco leaves and a theoretical basis and idea for optimization of the flue-curing technologies. By investigating tobacco variety K326, the tests were carried out for comparing the conventional flue-curing procedure and dry-ball temperature set and wet-ball temperature degradation flue-curing procedure. Based on the culture-independent approach and high-throughput sequencing procedure, the relationship between the flue-curing procedure for tobaccos and microbial communities in tobaccos was revealed by measuring the dynamic change of microbial communities. The results indicated that:(1) Relative to surface wiping method, washing method was more suitable for the sampling of microbes on the surface of tobacco leaves; (2) Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure was more favorable for maintaining the microbial diversity of tobaccos; (3) Relative to bacteria of the tobaccos, the succession rule of the fungal communities in tobaccos was relatively steady; (4)Compared with bacterial community diversity, the fungal community diversity presented an obvious negative correlation with temperature and humidity during the flue-curing process. (5) The function of bacterial communities in tobaccos matched with the material transformation law of tobaccos, having a direct correlation on the flue-curing process. In short, Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure can more favorably maintain the microbial diversity of tobaccos; moreover, the function of the tobacco system involved in microbes in tobaccos was closely related to the material transformation law of tobaccos in the flue-curing process. It validated that the bacteria in tobaccos play an important role in the flue-curing process of tobaccos.Binbin HuKaiyuan GuJiangshiqi GongKe ZhangDan ChenXian HeYi ChenKaixian GaoYan JinKun HuangYanmei ZhuCongming ZouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
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Medicine R Science Q Binbin Hu Kaiyuan Gu Jiangshiqi Gong Ke Zhang Dan Chen Xian He Yi Chen Kaixian Gao Yan Jin Kun Huang Yanmei Zhu Congming Zou The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| description |
Abstract The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process. It expects to provide a theoretical basis for the application of microbes in tobacco leaves and a theoretical basis and idea for optimization of the flue-curing technologies. By investigating tobacco variety K326, the tests were carried out for comparing the conventional flue-curing procedure and dry-ball temperature set and wet-ball temperature degradation flue-curing procedure. Based on the culture-independent approach and high-throughput sequencing procedure, the relationship between the flue-curing procedure for tobaccos and microbial communities in tobaccos was revealed by measuring the dynamic change of microbial communities. The results indicated that:(1) Relative to surface wiping method, washing method was more suitable for the sampling of microbes on the surface of tobacco leaves; (2) Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure was more favorable for maintaining the microbial diversity of tobaccos; (3) Relative to bacteria of the tobaccos, the succession rule of the fungal communities in tobaccos was relatively steady; (4)Compared with bacterial community diversity, the fungal community diversity presented an obvious negative correlation with temperature and humidity during the flue-curing process. (5) The function of bacterial communities in tobaccos matched with the material transformation law of tobaccos, having a direct correlation on the flue-curing process. In short, Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure can more favorably maintain the microbial diversity of tobaccos; moreover, the function of the tobacco system involved in microbes in tobaccos was closely related to the material transformation law of tobaccos in the flue-curing process. It validated that the bacteria in tobaccos play an important role in the flue-curing process of tobaccos. |
| format |
article |
| author |
Binbin Hu Kaiyuan Gu Jiangshiqi Gong Ke Zhang Dan Chen Xian He Yi Chen Kaixian Gao Yan Jin Kun Huang Yanmei Zhu Congming Zou |
| author_facet |
Binbin Hu Kaiyuan Gu Jiangshiqi Gong Ke Zhang Dan Chen Xian He Yi Chen Kaixian Gao Yan Jin Kun Huang Yanmei Zhu Congming Zou |
| author_sort |
Binbin Hu |
| title |
The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| title_short |
The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| title_full |
The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| title_fullStr |
The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| title_full_unstemmed |
The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| title_sort |
effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/21a309039e904bc4b646178b22b5b84a |
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