Flatbed epi relief-contrast cellular monitoring system for stable cell culture

Abstract Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tatsuya Osaki, Tatsuto Kageyama, Yuka Shimazu, Dina Mysnikova, Shintaro Takahashi, Shinichi Takimoto, Junji Fukuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21a966b8690143f2ac48becbeded81a9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:21a966b8690143f2ac48becbeded81a9
record_format	dspace
spelling	oai:doaj.org-article:21a966b8690143f2ac48becbeded81a92021-12-02T15:06:01ZFlatbed epi relief-contrast cellular monitoring system for stable cell culture10.1038/s41598-017-02001-x2045-2322https://doaj.org/article/21a966b8690143f2ac48becbeded81a92017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02001-xhttps://doaj.org/toc/2045-2322Abstract Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplasma or other types of cells, have compromised the reproducibility and reliability of cell-based approaches. Here, we propose an epi relief-contrast cellular monitoring system (eRC-CMS) that allows images of cells in a typical culture plate to be acquired, stored, and analysed for daily cell quality control. Due to its full flatbed nature and automated system, cells placed at any location on the stage can be analysed without special attention. Using this system, changes in the size, circularity, and proliferation of endothelial cells in subculture were recorded. Analyses of images of ~9,930,000 individual cells revealed that the growth activity and cell circularity in subcultures were closely correlated with their angiogenic activity in a subsequent hydrogel assay, demonstrating that eRC-CMS is useful for assessing cell quality in advance. We further demonstrated that eRC-CMS was feasible for the imaging of neurite elongation and spheroid formation. This system may provide a robust and versatile approach for daily cell preparation to facilitate reliable and reproducible cell-based studies.Tatsuya OsakiTatsuto KageyamaYuka ShimazuDina MysnikovaShintaro TakahashiShinichi TakimotoJunji FukudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatsuya Osaki Tatsuto Kageyama Yuka Shimazu Dina Mysnikova Shintaro Takahashi Shinichi Takimoto Junji Fukuda Flatbed epi relief-contrast cellular monitoring system for stable cell culture
description	Abstract Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplasma or other types of cells, have compromised the reproducibility and reliability of cell-based approaches. Here, we propose an epi relief-contrast cellular monitoring system (eRC-CMS) that allows images of cells in a typical culture plate to be acquired, stored, and analysed for daily cell quality control. Due to its full flatbed nature and automated system, cells placed at any location on the stage can be analysed without special attention. Using this system, changes in the size, circularity, and proliferation of endothelial cells in subculture were recorded. Analyses of images of ~9,930,000 individual cells revealed that the growth activity and cell circularity in subcultures were closely correlated with their angiogenic activity in a subsequent hydrogel assay, demonstrating that eRC-CMS is useful for assessing cell quality in advance. We further demonstrated that eRC-CMS was feasible for the imaging of neurite elongation and spheroid formation. This system may provide a robust and versatile approach for daily cell preparation to facilitate reliable and reproducible cell-based studies.
format	article
author	Tatsuya Osaki Tatsuto Kageyama Yuka Shimazu Dina Mysnikova Shintaro Takahashi Shinichi Takimoto Junji Fukuda
author_facet	Tatsuya Osaki Tatsuto Kageyama Yuka Shimazu Dina Mysnikova Shintaro Takahashi Shinichi Takimoto Junji Fukuda
author_sort	Tatsuya Osaki
title	Flatbed epi relief-contrast cellular monitoring system for stable cell culture
title_short	Flatbed epi relief-contrast cellular monitoring system for stable cell culture
title_full	Flatbed epi relief-contrast cellular monitoring system for stable cell culture
title_fullStr	Flatbed epi relief-contrast cellular monitoring system for stable cell culture
title_full_unstemmed	Flatbed epi relief-contrast cellular monitoring system for stable cell culture
title_sort	flatbed epi relief-contrast cellular monitoring system for stable cell culture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/21a966b8690143f2ac48becbeded81a9
work_keys_str_mv	AT tatsuyaosaki flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT tatsutokageyama flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT yukashimazu flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT dinamysnikova flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT shintarotakahashi flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT shinichitakimoto flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture AT junjifukuda flatbedepireliefcontrastcellularmonitoringsystemforstablecellculture
_version_	1718388630314024960

Flatbed epi relief-contrast cellular monitoring system for stable cell culture

Ejemplares similares