Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate

Abstract Triphenyl phosphate (TPHP) is one of the most highly utilized organophosphorus flame retardants, and has been frequently detected in various environmental matrices, including soil. So far, limited information is known regarding the potential toxicity of TPHP to the earthworm-soil ecosystem....

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Autores principales:	Lei Wang, Xulei Huang, Anna Karen Carrasco Laserna, Sam Fong Yau Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Triphenyl Phosphate (TPHP) Earthworms Thiol Conjugates Endogenous Metabolome Glucoside Conjugates Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21aa76ab08c143eba2e33a084eade5d1
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spelling	oai:doaj.org-article:21aa76ab08c143eba2e33a084eade5d12021-12-02T15:08:13ZUntargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate10.1038/s41598-018-34814-92045-2322https://doaj.org/article/21aa76ab08c143eba2e33a084eade5d12018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34814-9https://doaj.org/toc/2045-2322Abstract Triphenyl phosphate (TPHP) is one of the most highly utilized organophosphorus flame retardants, and has been frequently detected in various environmental matrices, including soil. So far, limited information is known regarding the potential toxicity of TPHP to the earthworm-soil ecosystem. We investigated the metabolism of TPHP and the perturbation of the endogenous metabolome in the earthworm, Perionyx excavatus, using gas chromatography mass spectrometry (GC-MS) and liquid chromatography quadrupole time-of-flight (LC-QTOF)-based untargeted metabolomics approach after acute exposure to TPHP for one and two days through a filter paper contact test, as well as after chronic exposure for 28 days in a soil microcosm experiment. TPHP showed low bioaccumulation potential in the earthworm-soil ecosystem at concentrations of 10 mg/kg and 50 mg/kg. Identified phase I metabolites include diphenyl phosphate, mono-hydroxylated and di-hydroxylated TPHP. Two groups of phase II metabolites, thiol conjugates (including mercaptolactic acid, cysteine, cysteinylglycine, and mercaptoethanol conjugates) and glucoside conjugates (including glucoside, glucoside-phosphate, and C14H19O10P conjugates), were putatively identified. Only acute TPHP exposure caused significant perturbations of the endogenous metabolome in earthworms, featuring fluctuations in amino acids, glucose, inosine and phospholipids. These results reveal novel phase II metabolism and toxicity of TPHP in P. excavatus.Lei WangXulei HuangAnna Karen Carrasco LasernaSam Fong Yau LiNature PortfolioarticleTriphenyl Phosphate (TPHP)EarthwormsThiol ConjugatesEndogenous MetabolomeGlucoside ConjugatesMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Triphenyl Phosphate (TPHP) Earthworms Thiol Conjugates Endogenous Metabolome Glucoside Conjugates Medicine R Science Q
spellingShingle	Triphenyl Phosphate (TPHP) Earthworms Thiol Conjugates Endogenous Metabolome Glucoside Conjugates Medicine R Science Q Lei Wang Xulei Huang Anna Karen Carrasco Laserna Sam Fong Yau Li Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
description	Abstract Triphenyl phosphate (TPHP) is one of the most highly utilized organophosphorus flame retardants, and has been frequently detected in various environmental matrices, including soil. So far, limited information is known regarding the potential toxicity of TPHP to the earthworm-soil ecosystem. We investigated the metabolism of TPHP and the perturbation of the endogenous metabolome in the earthworm, Perionyx excavatus, using gas chromatography mass spectrometry (GC-MS) and liquid chromatography quadrupole time-of-flight (LC-QTOF)-based untargeted metabolomics approach after acute exposure to TPHP for one and two days through a filter paper contact test, as well as after chronic exposure for 28 days in a soil microcosm experiment. TPHP showed low bioaccumulation potential in the earthworm-soil ecosystem at concentrations of 10 mg/kg and 50 mg/kg. Identified phase I metabolites include diphenyl phosphate, mono-hydroxylated and di-hydroxylated TPHP. Two groups of phase II metabolites, thiol conjugates (including mercaptolactic acid, cysteine, cysteinylglycine, and mercaptoethanol conjugates) and glucoside conjugates (including glucoside, glucoside-phosphate, and C14H19O10P conjugates), were putatively identified. Only acute TPHP exposure caused significant perturbations of the endogenous metabolome in earthworms, featuring fluctuations in amino acids, glucose, inosine and phospholipids. These results reveal novel phase II metabolism and toxicity of TPHP in P. excavatus.
format	article
author	Lei Wang Xulei Huang Anna Karen Carrasco Laserna Sam Fong Yau Li
author_facet	Lei Wang Xulei Huang Anna Karen Carrasco Laserna Sam Fong Yau Li
author_sort	Lei Wang
title	Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
title_short	Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
title_full	Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
title_fullStr	Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
title_full_unstemmed	Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate
title_sort	untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm perionyx excavatus after exposure to triphenyl phosphate
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/21aa76ab08c143eba2e33a084eade5d1
work_keys_str_mv	AT leiwang untargetedmetabolomicsrevealstransformationpathwaysandmetabolicresponseoftheearthwormperionyxexcavatusafterexposuretotriphenylphosphate AT xuleihuang untargetedmetabolomicsrevealstransformationpathwaysandmetabolicresponseoftheearthwormperionyxexcavatusafterexposuretotriphenylphosphate AT annakarencarrascolaserna untargetedmetabolomicsrevealstransformationpathwaysandmetabolicresponseoftheearthwormperionyxexcavatusafterexposuretotriphenylphosphate AT samfongyauli untargetedmetabolomicsrevealstransformationpathwaysandmetabolicresponseoftheearthwormperionyxexcavatusafterexposuretotriphenylphosphate
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Untargeted metabolomics reveals transformation pathways and metabolic response of the earthworm Perionyx excavatus after exposure to triphenyl phosphate

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