Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat

Abstract Silicon plays a vital role in plant growth. However, molecular mechanisms in response to silicon have not previously been studied in wheat. In this study, we used RNA-seq technology to identify differentially expressed genes (DEGs) in wheat seedlings treated with silicon. Results showed tha...

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Autores principales:	Lidong Hao, Shubing Shi, Haibin Guo, Jinshan Zhang, Peng Li, Yanfei Feng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21ad2267f12e4f43bb03778442b57a21
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spelling	oai:doaj.org-article:21ad2267f12e4f43bb03778442b57a212021-12-02T11:02:38ZTranscriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat10.1038/s41598-021-83912-82045-2322https://doaj.org/article/21ad2267f12e4f43bb03778442b57a212021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83912-8https://doaj.org/toc/2045-2322Abstract Silicon plays a vital role in plant growth. However, molecular mechanisms in response to silicon have not previously been studied in wheat. In this study, we used RNA-seq technology to identify differentially expressed genes (DEGs) in wheat seedlings treated with silicon. Results showed that many wheat genes responded to silicon treatment, including 3057 DEGs, of which 6.25% (191/3057) were predicted transcription factors (TFs). Approximately 14.67% (28 out of 191) of the differentially expressed TFs belonged to the MYB TF family. Gene ontology (GO) enrichment showed that the highly enriched DEGs were responsible for secondary biosynthetic processes. According to KEGG pathway analysis, the DEGs were related to chaperones and folding catalysts, phenylpropanoid biosynthesis, and protein processing in the endoplasmic reticulum. Moreover, 411 R2R3-MYB TFs were identified in the wheat genome, all of which were classified into 15 groups and accordingly named S1–S15. Among them, 28 were down-regulated under silicon treatment. This study revealed the essential role of MYB TFs in the silicon response mechanism of plants, and provides important genetic resources for breeding silicon-tolerant wheat.Lidong HaoShubing ShiHaibin GuoJinshan ZhangPeng LiYanfei FengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lidong Hao Shubing Shi Haibin Guo Jinshan Zhang Peng Li Yanfei Feng Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
description	Abstract Silicon plays a vital role in plant growth. However, molecular mechanisms in response to silicon have not previously been studied in wheat. In this study, we used RNA-seq technology to identify differentially expressed genes (DEGs) in wheat seedlings treated with silicon. Results showed that many wheat genes responded to silicon treatment, including 3057 DEGs, of which 6.25% (191/3057) were predicted transcription factors (TFs). Approximately 14.67% (28 out of 191) of the differentially expressed TFs belonged to the MYB TF family. Gene ontology (GO) enrichment showed that the highly enriched DEGs were responsible for secondary biosynthetic processes. According to KEGG pathway analysis, the DEGs were related to chaperones and folding catalysts, phenylpropanoid biosynthesis, and protein processing in the endoplasmic reticulum. Moreover, 411 R2R3-MYB TFs were identified in the wheat genome, all of which were classified into 15 groups and accordingly named S1–S15. Among them, 28 were down-regulated under silicon treatment. This study revealed the essential role of MYB TFs in the silicon response mechanism of plants, and provides important genetic resources for breeding silicon-tolerant wheat.
format	article
author	Lidong Hao Shubing Shi Haibin Guo Jinshan Zhang Peng Li Yanfei Feng
author_facet	Lidong Hao Shubing Shi Haibin Guo Jinshan Zhang Peng Li Yanfei Feng
author_sort	Lidong Hao
title	Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
title_short	Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
title_full	Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
title_fullStr	Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
title_full_unstemmed	Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat
title_sort	transcriptome analysis reveals differentially expressed myb transcription factors associated with silicon response in wheat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/21ad2267f12e4f43bb03778442b57a21
work_keys_str_mv	AT lidonghao transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat AT shubingshi transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat AT haibinguo transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat AT jinshanzhang transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat AT pengli transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat AT yanfeifeng transcriptomeanalysisrevealsdifferentiallyexpressedmybtranscriptionfactorsassociatedwithsiliconresponseinwheat
_version_	1718396278971301888

Transcriptome analysis reveals differentially expressed MYB transcription factors associated with silicon response in wheat

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