Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Xiaoran Yu,1,2,* Ruogu Xu,1,2,* Zhengchuan Zhang,1,2 Qiming Jiang,1,2 Yun Liu,1,2 Xiaolin Yu,1,2 Feilong Deng1,2 1Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People’s Republic of China; 2Gua...

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Autores principales:	Yu X, Xu R, Zhang Z, Jiang Q, Liu Y, Deng F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	multifunctional surface modifications osteoclastogenesis osteogenesis custom-made titanium bone substitutes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/21aff2f4956d44d596212256a9c371a0
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spelling	oai:doaj.org-article:21aff2f4956d44d596212256a9c371a02021-12-02T15:00:14ZDifferent Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration1178-2013https://doaj.org/article/21aff2f4956d44d596212256a9c371a02021-05-01T00:00:00Zhttps://www.dovepress.com/different-cell-and-tissue-behavior-of-micro-nano-tubes-and-micro-nano--peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Xiaoran Yu,1,2,* Ruogu Xu,1,2,* Zhengchuan Zhang,1,2 Qiming Jiang,1,2 Yun Liu,1,2 Xiaolin Yu,1,2 Feilong Deng1,2 1Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feilong Deng; Xiaolin YuDepartment of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, No. 56, Ling Yuan Xi Road, Guangzhou, 510055, People’s Republic of ChinaTel +86 20 83862537Fax +86 20 83822807Email dengfl@mail.sysu.edu.cn; yuxlin3@mail.sysu.edu.cnBackground and Purpose: Micro-/nano-tubes (TNTs) and micro-/nano-nets (TNNs) are the common and sensible choice in the first step of combined modifications of titanium surface for further functionalization in the purpose of extended indications and therapeutic effect. It is important to recognize the respective biologic reactions of these two substrates for guiding a biologically based first-step selection.Materials and Methods: TNTs were produced by anodic oxidation and TNNs were formed by alkali-heat treatment. The original selective laser melting (SLM) titanium surface was set as control. Surface characterization was evaluated by scanning electron microscopy, surface roughness, and water contact angle measurements. Osteoclastogenesis and osteogenesis were measured. MC3T3-E1 cells and RAW 264.7 cells were used for in vitro assay in terms of adhesion, proliferation, and differentiation. In vivo assessments were taken on Beagle dogs with micro-CT and histological analysis.Results: TNN and TNT groups performed decreased roughness and increased hydrophilicity compared with SLM group. For biological detections, the highest ALP activity and osteogenesis-related genes expression were observed in TNT group followed by TNN group (P < 0.05). Interestingly, when it comes to the osteoclastogenesis, TNNs displayed lowest TRAP activity and osteoclastogenesis-related genes expression and TNTs were lower than SLM but higher than TNNs (P < 0.05). BV/TV around implants was highest in TNT group after 4 weeks (P < 0.05). HE, ALP and TRAP staining showed that osteogenic and osteoclastic activity around TNTs were both higher than TNNs (P < 0.05).Conclusion: TNNs and TNTs have dual advantages in promotion of osteogenesis and inhibition of osteoclastogenesis. Furthermore, TNNs showed better capability in inhibiting osteoclast activity while TNTs facilitated stronger osteogenesis. Our results implied that TNT substrates would take advantage in early application after implantation, while diseases with inappropriate osteoclast activity would prefer TNN substrates, which will guide a biologically based first-step selection on combined modification for different clinical purposes.Keywords: multifunctional surface modifications, osteoclastogenesis, osteogenesis, custom-made titanium bone substitutesYu XXu RZhang ZJiang QLiu YYu XDeng FDove Medical Pressarticlemultifunctional surface modificationsosteoclastogenesisosteogenesiscustom-made titanium bone substitutesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3329-3342 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multifunctional surface modifications osteoclastogenesis osteogenesis custom-made titanium bone substitutes Medicine (General) R5-920
spellingShingle	multifunctional surface modifications osteoclastogenesis osteogenesis custom-made titanium bone substitutes Medicine (General) R5-920 Yu X Xu R Zhang Z Jiang Q Liu Y Yu X Deng F Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
description	Xiaoran Yu,1,2,* Ruogu Xu,1,2,* Zhengchuan Zhang,1,2 Qiming Jiang,1,2 Yun Liu,1,2 Xiaolin Yu,1,2 Feilong Deng1,2 1Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feilong Deng; Xiaolin YuDepartment of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, No. 56, Ling Yuan Xi Road, Guangzhou, 510055, People’s Republic of ChinaTel +86 20 83862537Fax +86 20 83822807Email dengfl@mail.sysu.edu.cn; yuxlin3@mail.sysu.edu.cnBackground and Purpose: Micro-/nano-tubes (TNTs) and micro-/nano-nets (TNNs) are the common and sensible choice in the first step of combined modifications of titanium surface for further functionalization in the purpose of extended indications and therapeutic effect. It is important to recognize the respective biologic reactions of these two substrates for guiding a biologically based first-step selection.Materials and Methods: TNTs were produced by anodic oxidation and TNNs were formed by alkali-heat treatment. The original selective laser melting (SLM) titanium surface was set as control. Surface characterization was evaluated by scanning electron microscopy, surface roughness, and water contact angle measurements. Osteoclastogenesis and osteogenesis were measured. MC3T3-E1 cells and RAW 264.7 cells were used for in vitro assay in terms of adhesion, proliferation, and differentiation. In vivo assessments were taken on Beagle dogs with micro-CT and histological analysis.Results: TNN and TNT groups performed decreased roughness and increased hydrophilicity compared with SLM group. For biological detections, the highest ALP activity and osteogenesis-related genes expression were observed in TNT group followed by TNN group (P < 0.05). Interestingly, when it comes to the osteoclastogenesis, TNNs displayed lowest TRAP activity and osteoclastogenesis-related genes expression and TNTs were lower than SLM but higher than TNNs (P < 0.05). BV/TV around implants was highest in TNT group after 4 weeks (P < 0.05). HE, ALP and TRAP staining showed that osteogenic and osteoclastic activity around TNTs were both higher than TNNs (P < 0.05).Conclusion: TNNs and TNTs have dual advantages in promotion of osteogenesis and inhibition of osteoclastogenesis. Furthermore, TNNs showed better capability in inhibiting osteoclast activity while TNTs facilitated stronger osteogenesis. Our results implied that TNT substrates would take advantage in early application after implantation, while diseases with inappropriate osteoclast activity would prefer TNN substrates, which will guide a biologically based first-step selection on combined modification for different clinical purposes.Keywords: multifunctional surface modifications, osteoclastogenesis, osteogenesis, custom-made titanium bone substitutes
format	article
author	Yu X Xu R Zhang Z Jiang Q Liu Y Yu X Deng F
author_facet	Yu X Xu R Zhang Z Jiang Q Liu Y Yu X Deng F
author_sort	Yu X
title	Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
title_short	Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
title_full	Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
title_fullStr	Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
title_full_unstemmed	Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration
title_sort	different cell and tissue behavior of micro-/nano-tubes and micro-/nano-nets topographies on selective laser melting titanium to enhance osseointegration
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/21aff2f4956d44d596212256a9c371a0
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Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

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