Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms

Abstract Lactic acid bacteria, in particular Lactococcus lactis, are widely used in the food industry, for the control and/or the protection of the manufacturing processes of fermented food. While L. lactis has been reported to form compact and uniform biofilms it was recently shown that certain str...

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Autores principales:	Ibrahima Drame, Christine Lafforgue, Cecile Formosa-Dague, Marie-Pierre Chapot-Chartier, Jean-Christophe Piard, Mickaël Castelain, Etienne Dague
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21b5d9550854410f9a13220dd5ed0ea8
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spelling	oai:doaj.org-article:21b5d9550854410f9a13220dd5ed0ea82021-12-02T15:53:02ZPili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms10.1038/s41598-021-84030-12045-2322https://doaj.org/article/21b5d9550854410f9a13220dd5ed0ea82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84030-1https://doaj.org/toc/2045-2322Abstract Lactic acid bacteria, in particular Lactococcus lactis, are widely used in the food industry, for the control and/or the protection of the manufacturing processes of fermented food. While L. lactis has been reported to form compact and uniform biofilms it was recently shown that certain strains able to display pili at their surface form more complex biofilms exhibiting heterogeneous and aerial structures. As the impact of those biofilm structures on the biomechanical properties of the biofilms is poorly understood, these were investigated using AFM force spectroscopy and imaging. Three types of strains were used i.e., a control strain devoid of pili and surface mucus-binding protein, a strain displaying pili but no mucus-binding proteins and a strain displaying both pili and a mucus-binding protein. To identify potential correlations between the nanomechanical measurements and the biofilm architecture, 24-h old biofilms were characterized by confocal laser scanning microscopy. Globally the strains devoid of pili displayed smoother and stiffer biofilms (Young Modulus of 4–100 kPa) than those of piliated strains (Young Modulus around 0.04–0.1 kPa). Additional display of a mucus-binding protein did not affect the biofilm stiffness but made the biofilm smoother and more compact. Finally, we demonstrated the role of pili in the biofilm cohesiveness by monitoring the homotypic adhesion of bacteria to the biofilm surface. These results will help to understand the role of pili and mucus-binding proteins withstanding external forces.Ibrahima DrameChristine LafforgueCecile Formosa-DagueMarie-Pierre Chapot-ChartierJean-Christophe PiardMickaël CastelainEtienne DagueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ibrahima Drame Christine Lafforgue Cecile Formosa-Dague Marie-Pierre Chapot-Chartier Jean-Christophe Piard Mickaël Castelain Etienne Dague Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
description	Abstract Lactic acid bacteria, in particular Lactococcus lactis, are widely used in the food industry, for the control and/or the protection of the manufacturing processes of fermented food. While L. lactis has been reported to form compact and uniform biofilms it was recently shown that certain strains able to display pili at their surface form more complex biofilms exhibiting heterogeneous and aerial structures. As the impact of those biofilm structures on the biomechanical properties of the biofilms is poorly understood, these were investigated using AFM force spectroscopy and imaging. Three types of strains were used i.e., a control strain devoid of pili and surface mucus-binding protein, a strain displaying pili but no mucus-binding proteins and a strain displaying both pili and a mucus-binding protein. To identify potential correlations between the nanomechanical measurements and the biofilm architecture, 24-h old biofilms were characterized by confocal laser scanning microscopy. Globally the strains devoid of pili displayed smoother and stiffer biofilms (Young Modulus of 4–100 kPa) than those of piliated strains (Young Modulus around 0.04–0.1 kPa). Additional display of a mucus-binding protein did not affect the biofilm stiffness but made the biofilm smoother and more compact. Finally, we demonstrated the role of pili in the biofilm cohesiveness by monitoring the homotypic adhesion of bacteria to the biofilm surface. These results will help to understand the role of pili and mucus-binding proteins withstanding external forces.
format	article
author	Ibrahima Drame Christine Lafforgue Cecile Formosa-Dague Marie-Pierre Chapot-Chartier Jean-Christophe Piard Mickaël Castelain Etienne Dague
author_facet	Ibrahima Drame Christine Lafforgue Cecile Formosa-Dague Marie-Pierre Chapot-Chartier Jean-Christophe Piard Mickaël Castelain Etienne Dague
author_sort	Ibrahima Drame
title	Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
title_short	Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
title_full	Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
title_fullStr	Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
title_full_unstemmed	Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms
title_sort	pili and other surface proteins influence the structure and the nanomechanical properties of lactococcus lactis biofilms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/21b5d9550854410f9a13220dd5ed0ea8
work_keys_str_mv	AT ibrahimadrame piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT christinelafforgue piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT cecileformosadague piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT mariepierrechapotchartier piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT jeanchristophepiard piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT mickaelcastelain piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms AT etiennedague piliandothersurfaceproteinsinfluencethestructureandthenanomechanicalpropertiesoflactococcuslactisbiofilms
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Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms

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