Dependency of hydration and growth conditions on the mechanical properties of oral biofilms

Abstract Within the oral cavity, dental biofilms experience dynamic environments, in part due to changes in dietary content, frequency of intake and health conditions. This can impact bacterial diversity and morpho-mechanical properties. While phenotypic properties of oral biofilms are closely relat...

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Autores principales:	J. Pattem, M. Davrandi, S. Aguayo, B. Slak, R. Maev, E. Allan, D. Spratt, L. Bozec
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21b69c33d66f428e8e55061663c74ed2
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spelling	oai:doaj.org-article:21b69c33d66f428e8e55061663c74ed22021-12-02T16:27:44ZDependency of hydration and growth conditions on the mechanical properties of oral biofilms10.1038/s41598-021-95701-42045-2322https://doaj.org/article/21b69c33d66f428e8e55061663c74ed22021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95701-4https://doaj.org/toc/2045-2322Abstract Within the oral cavity, dental biofilms experience dynamic environments, in part due to changes in dietary content, frequency of intake and health conditions. This can impact bacterial diversity and morpho-mechanical properties. While phenotypic properties of oral biofilms are closely related to their composition, these can readily change according to dynamic variations in the growth environment and nutrient availability. Understanding the interlink between phenotypic properties, variable growth conditions, and community characterization is an essential requirement to develop structure–property relationships in oral-biofilms. In this study, the impact of two distinct growth media types with increasing richness on the properties of oral biofilms was assessed through a new combination of in-vitro time-lapse biophysical methods with microbiological assays. Oral biofilms grown in the enriched media composition presented a decrease in their pH, an increase in soluble EPS production, and a severe reduction in bacterial diversity. Additionally, enriched media conditions presented an increase in biofilm volumetric changes (upon hydration) as well as a reduction in elastic modulus upon indentation. With hydration time considered a major factor contributing to changes in biofilm mechanical properties, we have shown that it is less associated than media richness. Future investigations can now use this time-lapse approach, with a clearer focus on the extracellular matrix of oral biofilms dictating their morpho-mechanical properties.J. PattemM. DavrandiS. AguayoB. SlakR. MaevE. AllanD. SprattL. BozecNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. Pattem M. Davrandi S. Aguayo B. Slak R. Maev E. Allan D. Spratt L. Bozec Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
description	Abstract Within the oral cavity, dental biofilms experience dynamic environments, in part due to changes in dietary content, frequency of intake and health conditions. This can impact bacterial diversity and morpho-mechanical properties. While phenotypic properties of oral biofilms are closely related to their composition, these can readily change according to dynamic variations in the growth environment and nutrient availability. Understanding the interlink between phenotypic properties, variable growth conditions, and community characterization is an essential requirement to develop structure–property relationships in oral-biofilms. In this study, the impact of two distinct growth media types with increasing richness on the properties of oral biofilms was assessed through a new combination of in-vitro time-lapse biophysical methods with microbiological assays. Oral biofilms grown in the enriched media composition presented a decrease in their pH, an increase in soluble EPS production, and a severe reduction in bacterial diversity. Additionally, enriched media conditions presented an increase in biofilm volumetric changes (upon hydration) as well as a reduction in elastic modulus upon indentation. With hydration time considered a major factor contributing to changes in biofilm mechanical properties, we have shown that it is less associated than media richness. Future investigations can now use this time-lapse approach, with a clearer focus on the extracellular matrix of oral biofilms dictating their morpho-mechanical properties.
format	article
author	J. Pattem M. Davrandi S. Aguayo B. Slak R. Maev E. Allan D. Spratt L. Bozec
author_facet	J. Pattem M. Davrandi S. Aguayo B. Slak R. Maev E. Allan D. Spratt L. Bozec
author_sort	J. Pattem
title	Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
title_short	Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
title_full	Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
title_fullStr	Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
title_full_unstemmed	Dependency of hydration and growth conditions on the mechanical properties of oral biofilms
title_sort	dependency of hydration and growth conditions on the mechanical properties of oral biofilms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/21b69c33d66f428e8e55061663c74ed2
work_keys_str_mv	AT jpattem dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT mdavrandi dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT saguayo dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT bslak dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT rmaev dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT eallan dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT dspratt dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms AT lbozec dependencyofhydrationandgrowthconditionsonthemechanicalpropertiesoforalbiofilms
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Dependency of hydration and growth conditions on the mechanical properties of oral biofilms

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