Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol

Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christofori Maria Ratna Rini Nastiti, Florentinus Dika Octa Riswanto
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	box-behnken design formulation nanoemulsion resveratrol validation Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/21b9035e8d1b464884a9badb6b8dabd5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:21b9035e8d1b464884a9badb6b8dabd5
record_format	dspace
spelling	oai:doaj.org-article:21b9035e8d1b464884a9badb6b8dabd52021-12-02T18:38:04ZAnalytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol1411-94202460-157810.22146/ijc.63730https://doaj.org/article/21b9035e8d1b464884a9badb6b8dabd52021-08-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/63730https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability. To overcome the issues, RSV was formulated as a topical nanoemulsion. It is important to ensure the quality of the dosage form by evaluating RSV load in the nanoformulation and optimizing the formula. A reversed-phase HPLC method was developed and validated prior to the load determination of RSV in the nanoemulsion formulation. The composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® was further optimized by employing a Box-Behnken Design (BBD) to achieve the optimum composition with expected viscosity and RSV load. The HPLC method for determining RSV load was successfully validated for parameters of selectivity with the resolution of 8.487, linearity and range (r = 0.9979), precision (0.12% of RSD), accuracy (109–110% of recovery), the limit of detection (0.574 µg/mL), and limit of quantitation (1.740 µg/mL). The result of formula optimization was promising, showing the optimum composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® at 4.44 g, 30.97 g, and 11.39 g, respectively.Christofori Maria Ratna Rini NastitiFlorentinus Dika Octa RiswantoDepartment of Chemistry, Universitas Gadjah Madaarticlebox-behnken designformulationnanoemulsionresveratrolvalidationChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 5, Pp 1287-1297 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	box-behnken design formulation nanoemulsion resveratrol validation Chemistry QD1-999
spellingShingle	box-behnken design formulation nanoemulsion resveratrol validation Chemistry QD1-999 Christofori Maria Ratna Rini Nastiti Florentinus Dika Octa Riswanto Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
description	Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability. To overcome the issues, RSV was formulated as a topical nanoemulsion. It is important to ensure the quality of the dosage form by evaluating RSV load in the nanoformulation and optimizing the formula. A reversed-phase HPLC method was developed and validated prior to the load determination of RSV in the nanoemulsion formulation. The composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® was further optimized by employing a Box-Behnken Design (BBD) to achieve the optimum composition with expected viscosity and RSV load. The HPLC method for determining RSV load was successfully validated for parameters of selectivity with the resolution of 8.487, linearity and range (r = 0.9979), precision (0.12% of RSD), accuracy (109–110% of recovery), the limit of detection (0.574 µg/mL), and limit of quantitation (1.740 µg/mL). The result of formula optimization was promising, showing the optimum composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® at 4.44 g, 30.97 g, and 11.39 g, respectively.
format	article
author	Christofori Maria Ratna Rini Nastiti Florentinus Dika Octa Riswanto
author_facet	Christofori Maria Ratna Rini Nastiti Florentinus Dika Octa Riswanto
author_sort	Christofori Maria Ratna Rini Nastiti
title	Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
title_short	Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
title_full	Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
title_fullStr	Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
title_full_unstemmed	Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol
title_sort	analytical method validation and formula optimization of topical nanoemulsion formulation containing resveratrol
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/21b9035e8d1b464884a9badb6b8dabd5
work_keys_str_mv	AT christoforimariaratnarininastiti analyticalmethodvalidationandformulaoptimizationoftopicalnanoemulsionformulationcontainingresveratrol AT florentinusdikaoctariswanto analyticalmethodvalidationandformulaoptimizationoftopicalnanoemulsionformulationcontainingresveratrol
_version_	1718377727673761792

Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol

Ejemplares similares